Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3577 and GFF3578 are separated by 116 nucleotides GFF3578 and GFF3579 overlap by 4 nucleotides GFF3579 and GFF3580 overlap by 43 nucleotides
GFF3577 - Transcriptional regulator, LysR family, at 88,551 to 89,441
GFF3577
GFF3578 - Glutathione S-transferase, unnamed subgroup (EC 2.5.1.18), at 89,558 to 90,217
GFF3578
GFF3579 - probable iron-sulfur binding protein YPO1417, at 90,214 to 91,203
GFF3579
GFF3580 - COG2124: Cytochrome P450, at 91,161 to 92,330
GFF3580
Position (kb)
90
91
92 Strain fitness (log2 ratio)
-1
0
1
2 at 89.248 kb on + strand, within GFF3577 at 89.248 kb on + strand, within GFF3577 at 89.603 kb on + strand at 89.604 kb on - strand at 89.805 kb on - strand, within GFF3578 at 89.819 kb on + strand, within GFF3578 at 89.912 kb on + strand, within GFF3578 at 90.075 kb on - strand, within GFF3578 at 90.080 kb on + strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.081 kb on - strand, within GFF3578 at 90.351 kb on - strand, within GFF3579 at 90.640 kb on + strand, within GFF3579 at 90.641 kb on - strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.830 kb on + strand, within GFF3579 at 90.831 kb on - strand, within GFF3579 at 90.831 kb on - strand, within GFF3579
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 89,248 + GFF3577 0.78 +0.9 89,248 + GFF3577 0.78 +0.9 89,603 + -0.5 89,604 - -0.8 89,805 - GFF3578 0.37 -0.2 89,819 + GFF3578 0.40 +0.8 89,912 + GFF3578 0.54 +1.0 90,075 - GFF3578 0.78 -0.3 90,080 + GFF3578 0.79 -1.0 90,081 - GFF3578 0.79 -0.6 90,081 - GFF3578 0.79 -0.1 90,081 - GFF3578 0.79 -0.2 90,081 - GFF3578 0.79 -0.2 90,081 - GFF3578 0.79 +0.8 90,081 - GFF3578 0.79 -1.4 90,351 - GFF3579 0.14 +0.8 90,640 + GFF3579 0.43 -0.9 90,641 - GFF3579 0.43 -0.4 90,830 + GFF3579 0.62 -0.2 90,830 + GFF3579 0.62 +1.8 90,830 + GFF3579 0.62 +0.1 90,831 - GFF3579 0.62 -1.1 90,831 - GFF3579 0.62 -0.7
Or see this region's nucleotide sequence