Strain Fitness in Variovorax sp. SCN45 around GFF3423

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF3421 and GFF3422 are separated by 31 nucleotidesGFF3422 and GFF3423 are separated by 8 nucleotidesGFF3423 and GFF3424 are separated by 213 nucleotides GFF3421 - Acetoacetyl-CoA synthetase (EC 6.2.1.16), at 244,385 to 246,388 GFF3421 GFF3422 - Lipoprotein-related protein, at 246,420 to 246,842 GFF3422 GFF3423 - Adenylate cyclase (EC 4.6.1.1), at 246,851 to 248,785 GFF3423 GFF3424 - FIG00443816: hypothetical protein, at 248,999 to 249,709 GFF3424 Position (kb) 246 247 248 249Strain fitness (log2 ratio) -2 -1 0 1 2 3at 245.891 kb on + strand, within GFF3421at 245.892 kb on - strand, within GFF3421at 245.966 kb on + strand, within GFF3421at 245.966 kb on + strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 245.967 kb on - strand, within GFF3421at 246.026 kb on + strand, within GFF3421at 246.026 kb on + strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.027 kb on - strand, within GFF3421at 246.083 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.101 kb on + strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.102 kb on - strand, within GFF3421at 246.353 kb on + strandat 246.353 kb on + strandat 246.353 kb on + strandat 246.353 kb on + strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.354 kb on - strandat 246.386 kb on + strandat 246.407 kb on + strandat 246.408 kb on - strandat 246.664 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.709 kb on - strand, within GFF3422at 246.768 kb on + strand, within GFF3422at 246.768 kb on + strand, within GFF3422at 246.870 kb on + strandat 246.870 kb on + strandat 247.047 kb on + strand, within GFF3423at 247.048 kb on - strand, within GFF3423at 247.048 kb on - strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.203 kb on + strand, within GFF3423at 247.204 kb on - strand, within GFF3423at 247.204 kb on - strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.317 kb on + strand, within GFF3423at 247.318 kb on - strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.803 kb on + strand, within GFF3423at 247.804 kb on - strand, within GFF3423at 247.804 kb on - strand, within GFF3423at 247.818 kb on + strand, within GFF3423at 247.819 kb on - strand, within GFF3423at 247.819 kb on - strand, within GFF3423at 248.110 kb on - strand, within GFF3423at 248.110 kb on - strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.145 kb on + strand, within GFF3423at 248.146 kb on - strand, within GFF3423at 248.146 kb on - strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.190 kb on + strand, within GFF3423at 248.191 kb on - strand, within GFF3423at 248.191 kb on - strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.214 kb on + strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.215 kb on - strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.250 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.292 kb on + strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.293 kb on - strand, within GFF3423at 248.356 kb on - strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.397 kb on + strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.398 kb on - strand, within GFF3423at 248.582 kb on + strand, within GFF3423at 248.619 kb on + strandat 248.619 kb on + strandat 248.620 kb on - strandat 248.620 kb on - strandat 248.684 kb on + strandat 248.684 kb on + strandat 248.684 kb on + strandat 248.685 kb on - strandat 248.685 kb on - strandat 248.685 kb on - strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.785 kb on + strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 248.786 kb on - strandat 249.031 kb on + strandat 249.032 kb on - strandat 249.032 kb on - strandat 249.084 kb on + strand, within GFF3424at 249.085 kb on - strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.303 kb on + strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.304 kb on - strand, within GFF3424at 249.319 kb on - strand, within GFF3424at 249.375 kb on + strand, within GFF3424at 249.376 kb on - strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.456 kb on + strand, within GFF3424at 249.457 kb on - strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.507 kb on + strand, within GFF3424at 249.508 kb on - strand, within GFF3424at 249.508 kb on - strand, within GFF3424at 249.513 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.579 kb on + strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424at 249.580 kb on - strand, within GFF3424

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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245,891 + GFF3421 0.75 +0.1
245,892 - GFF3421 0.75 -0.5
245,966 + GFF3421 0.79 +0.2
245,966 + GFF3421 0.79 -1.1
245,967 - GFF3421 0.79 -1.5
245,967 - GFF3421 0.79 +0.5
245,967 - GFF3421 0.79 -0.1
246,026 + GFF3421 0.82 -1.5
246,026 + GFF3421 0.82 -0.2
246,027 - GFF3421 0.82 +0.9
246,027 - GFF3421 0.82 -0.3
246,027 - GFF3421 0.82 +0.0
246,027 - GFF3421 0.82 -0.8
246,027 - GFF3421 0.82 +1.3
246,027 - GFF3421 0.82 +0.7
246,027 - GFF3421 0.82 -0.3
246,027 - GFF3421 0.82 +0.2
246,027 - GFF3421 0.82 -1.0
246,027 - GFF3421 0.82 +1.3
246,083 + GFF3421 0.85 -0.2
246,101 + GFF3421 0.86 -0.1
246,101 + GFF3421 0.86 -0.7
246,101 + GFF3421 0.86 +0.6
246,101 + GFF3421 0.86 -2.0
246,101 + GFF3421 0.86 -0.5
246,101 + GFF3421 0.86 -0.2
246,102 - GFF3421 0.86 +0.6
246,102 - GFF3421 0.86 -0.1
246,102 - GFF3421 0.86 +0.6
246,102 - GFF3421 0.86 -0.8
246,102 - GFF3421 0.86 -1.5
246,102 - GFF3421 0.86 -0.9
246,102 - GFF3421 0.86 -0.4
246,353 + +0.6
246,353 + +0.9
246,353 + +0.1
246,353 + +0.3
246,354 - +0.7
246,354 - -1.0
246,354 - -0.2
246,354 - +0.1
246,354 - -0.4
246,354 - -0.6
246,354 - +0.3
246,354 - -0.4
246,354 - -0.0
246,386 + -0.1
246,407 + -0.4
246,408 - +0.5
246,664 - GFF3422 0.58 -1.0
246,709 - GFF3422 0.68 +0.5
246,709 - GFF3422 0.68 -0.5
246,709 - GFF3422 0.68 -0.9
246,768 + GFF3422 0.82 -0.2
246,768 + GFF3422 0.82 -1.1
246,870 + -1.1
246,870 + +0.6
247,047 + GFF3423 0.10 -1.0
247,048 - GFF3423 0.10 -0.6
247,048 - GFF3423 0.10 +3.1
247,203 + GFF3423 0.18 +1.3
247,203 + GFF3423 0.18 -0.8
247,203 + GFF3423 0.18 -1.1
247,204 - GFF3423 0.18 +2.5
247,204 - GFF3423 0.18 -0.2
247,317 + GFF3423 0.24 +1.0
247,317 + GFF3423 0.24 +0.4
247,317 + GFF3423 0.24 -1.1
247,318 - GFF3423 0.24 +0.6
247,803 + GFF3423 0.49 +0.2
247,803 + GFF3423 0.49 +1.1
247,803 + GFF3423 0.49 +1.2
247,803 + GFF3423 0.49 +2.9
247,803 + GFF3423 0.49 -0.0
247,803 + GFF3423 0.49 -0.2
247,803 + GFF3423 0.49 -1.4
247,804 - GFF3423 0.49 -1.2
247,804 - GFF3423 0.49 -0.2
247,818 + GFF3423 0.50 +0.1
247,819 - GFF3423 0.50 -0.0
247,819 - GFF3423 0.50 -0.1
248,110 - GFF3423 0.65 -1.8
248,110 - GFF3423 0.65 -0.0
248,145 + GFF3423 0.67 +1.3
248,145 + GFF3423 0.67 -0.6
248,145 + GFF3423 0.67 +1.9
248,145 + GFF3423 0.67 -0.1
248,146 - GFF3423 0.67 +0.4
248,146 - GFF3423 0.67 -0.7
248,190 + GFF3423 0.69 +0.2
248,190 + GFF3423 0.69 -2.1
248,190 + GFF3423 0.69 -1.5
248,190 + GFF3423 0.69 +0.8
248,191 - GFF3423 0.69 -1.7
248,191 - GFF3423 0.69 -1.8
248,214 + GFF3423 0.70 +0.3
248,214 + GFF3423 0.70 +0.6
248,214 + GFF3423 0.70 +0.5
248,214 + GFF3423 0.70 -0.3
248,215 - GFF3423 0.70 -0.6
248,215 - GFF3423 0.70 -0.4
248,215 - GFF3423 0.70 -0.5
248,215 - GFF3423 0.70 -0.6
248,250 + GFF3423 0.72 -2.0
248,250 + GFF3423 0.72 +0.9
248,250 + GFF3423 0.72 -0.4
248,292 + GFF3423 0.74 -0.9
248,292 + GFF3423 0.74 -0.4
248,292 + GFF3423 0.74 -0.7
248,293 - GFF3423 0.75 -0.8
248,293 - GFF3423 0.75 +0.9
248,293 - GFF3423 0.75 -0.3
248,356 - GFF3423 0.78 -0.4
248,397 + GFF3423 0.80 -0.2
248,397 + GFF3423 0.80 -1.0
248,397 + GFF3423 0.80 -0.3
248,398 - GFF3423 0.80 +0.3
248,398 - GFF3423 0.80 +0.5
248,398 - GFF3423 0.80 -1.2
248,398 - GFF3423 0.80 +0.6
248,398 - GFF3423 0.80 -0.8
248,582 + GFF3423 0.89 -0.3
248,619 + -0.2
248,619 + -1.0
248,620 - -0.3
248,620 - -0.5
248,684 + +0.2
248,684 + -0.6
248,684 + -0.1
248,685 - -1.7
248,685 - -0.8
248,685 - -2.1
248,785 + -0.1
248,785 + -0.0
248,785 + +0.9
248,785 + +0.6
248,786 - -1.2
248,786 - +1.2
248,786 - -0.4
248,786 - -0.4
248,786 - -0.9
248,786 - -0.7
249,031 + +0.7
249,032 - +0.2
249,032 - -0.5
249,084 + GFF3424 0.12 -0.3
249,085 - GFF3424 0.12 +0.7
249,303 + GFF3424 0.43 -0.0
249,303 + GFF3424 0.43 +0.8
249,303 + GFF3424 0.43 -1.2
249,304 - GFF3424 0.43 -0.3
249,304 - GFF3424 0.43 -0.6
249,304 - GFF3424 0.43 -0.3
249,319 - GFF3424 0.45 +0.1
249,375 + GFF3424 0.53 +0.4
249,376 - GFF3424 0.53 -0.6
249,456 + GFF3424 0.64 +0.2
249,456 + GFF3424 0.64 +0.2
249,456 + GFF3424 0.64 -0.7
249,457 - GFF3424 0.64 +0.0
249,507 + GFF3424 0.71 +0.0
249,507 + GFF3424 0.71 -0.2
249,507 + GFF3424 0.71 -0.9
249,508 - GFF3424 0.72 -0.6
249,508 - GFF3424 0.72 -0.2
249,513 + GFF3424 0.72 -1.4
249,579 + GFF3424 0.82 +0.1
249,579 + GFF3424 0.82 -0.5
249,579 + GFF3424 0.82 -0.3
249,579 + GFF3424 0.82 +0.5
249,579 + GFF3424 0.82 -1.0
249,579 + GFF3424 0.82 +0.3
249,579 + GFF3424 0.82 +1.5
249,579 + GFF3424 0.82 -0.3
249,580 - GFF3424 0.82 -1.3
249,580 - GFF3424 0.82 +0.0
249,580 - GFF3424 0.82 +0.0
249,580 - GFF3424 0.82 -1.1
249,580 - GFF3424 0.82 -0.6
249,580 - GFF3424 0.82 +1.7

Or see this region's nucleotide sequence