Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3370 and GFF3371 overlap by 17 nucleotides GFF3371 and GFF3372 overlap by 4 nucleotides GFF3372 and GFF3373 are separated by 66 nucleotides
GFF3370 - hypothetical protein, at 189,716 to 191,053
GFF3370
GFF3371 - FIG00456413: hypothetical protein, at 191,037 to 192,425
GFF3371
GFF3372 - hypothetical protein, at 192,422 to 192,874
GFF3372
GFF3373 - Hydroxymethylglutaryl-CoA reductase (EC 1.1.1.34), at 192,941 to 194,230
GFF3373
Position (kb) 191
192
193 Strain fitness (log2 ratio) -2
-1
0
1
2 at 190.215 kb on - strand, within GFF3370 at 190.215 kb on - strand, within GFF3370 at 190.282 kb on + strand, within GFF3370 at 190.283 kb on - strand, within GFF3370 at 190.709 kb on + strand, within GFF3370 at 190.730 kb on + strand, within GFF3370 at 190.730 kb on + strand, within GFF3370 at 190.730 kb on + strand, within GFF3370 at 190.730 kb on + strand, within GFF3370 at 190.731 kb on - strand, within GFF3370 at 190.731 kb on - strand, within GFF3370 at 190.731 kb on - strand, within GFF3370 at 190.763 kb on - strand, within GFF3370 at 190.857 kb on + strand, within GFF3370 at 190.857 kb on + strand, within GFF3370 at 191.038 kb on + strand at 191.038 kb on + strand at 191.039 kb on - strand at 191.083 kb on + strand at 191.083 kb on + strand at 191.084 kb on - strand at 191.084 kb on - strand at 191.084 kb on - strand at 191.084 kb on - strand at 191.086 kb on + strand at 191.086 kb on + strand at 191.086 kb on + strand at 191.086 kb on + strand at 191.087 kb on - strand at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.512 kb on + strand, within GFF3371 at 191.513 kb on - strand, within GFF3371 at 191.513 kb on - strand, within GFF3371 at 191.513 kb on - strand, within GFF3371 at 191.513 kb on - strand, within GFF3371 at 191.566 kb on + strand, within GFF3371 at 191.567 kb on - strand, within GFF3371 at 191.567 kb on - strand, within GFF3371 at 191.716 kb on + strand, within GFF3371 at 191.851 kb on + strand, within GFF3371 at 191.875 kb on + strand, within GFF3371 at 191.875 kb on + strand, within GFF3371 at 191.876 kb on - strand, within GFF3371 at 191.876 kb on - strand, within GFF3371 at 192.462 kb on + strand at 192.480 kb on + strand, within GFF3372 at 192.480 kb on + strand, within GFF3372 at 192.480 kb on + strand, within GFF3372 at 192.480 kb on + strand, within GFF3372 at 192.481 kb on - strand, within GFF3372 at 192.481 kb on - strand, within GFF3372 at 192.481 kb on - strand, within GFF3372 at 192.481 kb on - strand, within GFF3372 at 192.693 kb on + strand, within GFF3372 at 192.693 kb on + strand, within GFF3372 at 192.694 kb on - strand, within GFF3372 at 192.827 kb on + strand, within GFF3372 at 192.827 kb on + strand, within GFF3372 at 192.827 kb on + strand, within GFF3372 at 192.828 kb on - strand, within GFF3372 at 192.942 kb on + strand at 192.942 kb on + strand at 192.943 kb on - strand at 192.943 kb on - strand at 192.943 kb on - strand at 193.329 kb on + strand, within GFF3373 at 193.330 kb on - strand, within GFF3373 at 193.330 kb on - strand, within GFF3373 at 193.350 kb on + strand, within GFF3373 at 193.350 kb on + strand, within GFF3373
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 190,215 - GFF3370 0.37 +0.6 190,215 - GFF3370 0.37 -0.4 190,282 + GFF3370 0.42 -0.0 190,283 - GFF3370 0.42 -0.0 190,709 + GFF3370 0.74 -0.8 190,730 + GFF3370 0.76 -0.5 190,730 + GFF3370 0.76 +0.2 190,730 + GFF3370 0.76 +0.9 190,730 + GFF3370 0.76 -0.9 190,731 - GFF3370 0.76 -0.9 190,731 - GFF3370 0.76 -0.2 190,731 - GFF3370 0.76 +0.8 190,763 - GFF3370 0.78 -0.4 190,857 + GFF3370 0.85 +0.6 190,857 + GFF3370 0.85 -0.9 191,038 + -0.9 191,038 + -1.6 191,039 - +1.9 191,083 + -0.2 191,083 + +0.0 191,084 - +0.8 191,084 - +0.1 191,084 - +0.6 191,084 - -0.4 191,086 + +0.0 191,086 + -0.2 191,086 + +0.2 191,086 + +0.1 191,087 - -0.8 191,512 + GFF3371 0.34 -0.4 191,512 + GFF3371 0.34 -0.1 191,512 + GFF3371 0.34 -0.3 191,512 + GFF3371 0.34 +0.7 191,512 + GFF3371 0.34 -0.7 191,512 + GFF3371 0.34 +0.8 191,512 + GFF3371 0.34 -0.2 191,512 + GFF3371 0.34 -0.4 191,512 + GFF3371 0.34 -0.4 191,512 + GFF3371 0.34 -1.3 191,513 - GFF3371 0.34 +0.1 191,513 - GFF3371 0.34 -1.2 191,513 - GFF3371 0.34 -0.3 191,513 - GFF3371 0.34 -1.1 191,566 + GFF3371 0.38 -0.6 191,567 - GFF3371 0.38 +0.2 191,567 - GFF3371 0.38 +0.2 191,716 + GFF3371 0.49 -0.3 191,851 + GFF3371 0.59 -1.5 191,875 + GFF3371 0.60 +0.1 191,875 + GFF3371 0.60 -0.1 191,876 - GFF3371 0.60 -1.1 191,876 - GFF3371 0.60 -0.1 192,462 + -1.6 192,480 + GFF3372 0.13 +0.0 192,480 + GFF3372 0.13 -0.2 192,480 + GFF3372 0.13 -0.9 192,480 + GFF3372 0.13 -1.5 192,481 - GFF3372 0.13 -0.1 192,481 - GFF3372 0.13 -1.4 192,481 - GFF3372 0.13 +0.6 192,481 - GFF3372 0.13 -1.2 192,693 + GFF3372 0.60 -0.0 192,693 + GFF3372 0.60 -0.2 192,694 - GFF3372 0.60 +0.9 192,827 + GFF3372 0.89 +1.2 192,827 + GFF3372 0.89 -0.3 192,827 + GFF3372 0.89 -0.3 192,828 - GFF3372 0.90 -0.7 192,942 + -1.1 192,942 + -1.0 192,943 - +0.5 192,943 - -0.6 192,943 - +0.4 193,329 + GFF3373 0.30 -1.4 193,330 - GFF3373 0.30 +0.8 193,330 - GFF3373 0.30 -2.1 193,350 + GFF3373 0.32 +0.3 193,350 + GFF3373 0.32 -1.1
Or see this region's nucleotide sequence