Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3351 and GFF3352 overlap by 17 nucleotides GFF3352 and GFF3353 overlap by 4 nucleotides GFF3353 and GFF3354 are separated by 31 nucleotides GFF3354 and GFF3355 are separated by 19 nucleotides
GFF3351 - FIG00458971: hypothetical protein, at 174,393 to 174,950
GFF3351
GFF3352 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 174,934 to 175,776
GFF3352
GFF3353 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 175,773 to 176,618
GFF3353
GFF3354 - FIG00537998: hypothetical protein, at 176,650 to 177,546
GFF3354
GFF3355 - no description, at 177,566 to 177,958
GFF3355
Position (kb) 175
176
177 Strain fitness (log2 ratio) -1
0
1 at 174.780 kb on + strand, within GFF3351 at 175.435 kb on + strand, within GFF3352 at 175.436 kb on - strand, within GFF3352 at 175.729 kb on + strand at 175.730 kb on - strand at 175.730 kb on - strand at 175.921 kb on - strand, within GFF3353 at 176.394 kb on + strand, within GFF3353 at 176.395 kb on - strand, within GFF3353 at 176.395 kb on - strand, within GFF3353 at 176.502 kb on + strand, within GFF3353 at 176.502 kb on + strand, within GFF3353 at 176.502 kb on + strand, within GFF3353 at 176.502 kb on + strand, within GFF3353 at 176.503 kb on - strand, within GFF3353 at 176.503 kb on - strand, within GFF3353 at 176.503 kb on - strand, within GFF3353 at 176.503 kb on - strand, within GFF3353 at 176.570 kb on - strand at 176.580 kb on + strand at 176.581 kb on - strand at 176.803 kb on + strand, within GFF3354 at 176.803 kb on + strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 176.804 kb on - strand, within GFF3354 at 177.383 kb on - strand, within GFF3354 at 177.544 kb on + strand at 177.545 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 174,780 + GFF3351 0.69 +1.5 175,435 + GFF3352 0.59 +0.9 175,436 - GFF3352 0.60 -0.1 175,729 + -0.1 175,730 - +1.1 175,730 - +0.2 175,921 - GFF3353 0.17 -1.7 176,394 + GFF3353 0.73 -0.5 176,395 - GFF3353 0.74 -0.4 176,395 - GFF3353 0.74 -0.2 176,502 + GFF3353 0.86 -1.0 176,502 + GFF3353 0.86 -0.6 176,502 + GFF3353 0.86 -0.1 176,502 + GFF3353 0.86 -0.4 176,503 - GFF3353 0.86 -0.3 176,503 - GFF3353 0.86 -0.2 176,503 - GFF3353 0.86 +0.9 176,503 - GFF3353 0.86 +0.9 176,570 - -0.4 176,580 + -0.7 176,581 - -0.7 176,803 + GFF3354 0.17 -0.3 176,803 + GFF3354 0.17 -0.6 176,804 - GFF3354 0.17 -0.0 176,804 - GFF3354 0.17 -1.5 176,804 - GFF3354 0.17 -1.2 176,804 - GFF3354 0.17 -0.5 177,383 - GFF3354 0.82 -1.1 177,544 + +0.9 177,545 - -0.4
Or see this region's nucleotide sequence