Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3350 and GFF3351 are separated by 14 nucleotides GFF3351 and GFF3352 overlap by 17 nucleotides GFF3352 and GFF3353 overlap by 4 nucleotides
GFF3350 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate) / ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 172,549 to 174,378
GFF3350
GFF3351 - FIG00458971: hypothetical protein, at 174,393 to 174,950
GFF3351
GFF3352 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 174,934 to 175,776
GFF3352
GFF3353 - Branched-chain amino acid ABC transporter, ATP-binding protein LivF (TC 3.A.1.4.1), at 175,773 to 176,618
GFF3353
Position (kb) 174
175 Strain fitness (log2 ratio) -1
0
1 at 173.545 kb on + strand, within GFF3350 at 173.546 kb on - strand, within GFF3350 at 173.546 kb on - strand, within GFF3350 at 173.579 kb on - strand, within GFF3350 at 173.669 kb on - strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.794 kb on + strand, within GFF3350 at 173.795 kb on - strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.941 kb on + strand, within GFF3350 at 173.942 kb on - strand, within GFF3350 at 174.079 kb on + strand, within GFF3350 at 174.080 kb on - strand, within GFF3350 at 174.148 kb on + strand, within GFF3350 at 174.148 kb on + strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.149 kb on - strand, within GFF3350 at 174.154 kb on + strand, within GFF3350 at 174.154 kb on + strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.155 kb on - strand, within GFF3350 at 174.317 kb on - strand at 174.336 kb on - strand at 174.411 kb on + strand at 174.412 kb on - strand at 174.412 kb on - strand at 174.585 kb on + strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.586 kb on - strand, within GFF3351 at 174.780 kb on + strand, within GFF3351 at 175.435 kb on + strand, within GFF3352 at 175.436 kb on - strand, within GFF3352 at 175.729 kb on + strand at 175.730 kb on - strand at 175.730 kb on - strand at 175.921 kb on - strand, within GFF3353
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 173,545 + GFF3350 0.54 -0.7 173,546 - GFF3350 0.54 -0.4 173,546 - GFF3350 0.54 +0.6 173,579 - GFF3350 0.56 -0.6 173,669 - GFF3350 0.61 -1.1 173,794 + GFF3350 0.68 -1.2 173,794 + GFF3350 0.68 -0.8 173,794 + GFF3350 0.68 +0.3 173,794 + GFF3350 0.68 +0.2 173,795 - GFF3350 0.68 -1.3 173,941 + GFF3350 0.76 -1.5 173,941 + GFF3350 0.76 -0.5 173,941 + GFF3350 0.76 +0.4 173,942 - GFF3350 0.76 -0.1 174,079 + GFF3350 0.84 +0.6 174,080 - GFF3350 0.84 -0.3 174,148 + GFF3350 0.87 +0.1 174,148 + GFF3350 0.87 -0.3 174,149 - GFF3350 0.87 -0.3 174,149 - GFF3350 0.87 -1.3 174,149 - GFF3350 0.87 -0.3 174,154 + GFF3350 0.88 -1.5 174,154 + GFF3350 0.88 -0.5 174,155 - GFF3350 0.88 -0.0 174,155 - GFF3350 0.88 -0.8 174,155 - GFF3350 0.88 -0.1 174,317 - +0.9 174,336 - -1.4 174,411 + -0.0 174,412 - -0.4 174,412 - -0.4 174,585 + GFF3351 0.34 +0.3 174,586 - GFF3351 0.35 +0.2 174,586 - GFF3351 0.35 +0.3 174,586 - GFF3351 0.35 -1.1 174,780 + GFF3351 0.69 +1.5 175,435 + GFF3352 0.59 +0.9 175,436 - GFF3352 0.60 -0.1 175,729 + -0.1 175,730 - +1.1 175,730 - +0.2 175,921 - GFF3353 0.17 -1.7
Or see this region's nucleotide sequence