Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3326 and GFF3327 overlap by 4 nucleotides GFF3327 and GFF3328 are separated by 155 nucleotides GFF3328 and GFF3329 are separated by 85 nucleotides
GFF3326 - hypothetical protein, at 152,762 to 153,223
GFF3326
GFF3327 - RidA/YER057c/UK114 superfamily, group 1, at 153,220 to 153,705
GFF3327
GFF3328 - NADH:flavin oxidoreductases, Old Yellow Enzyme family, at 153,861 to 154,964
GFF3328
GFF3329 - Terpene utilization protein AtuA, at 155,050 to 156,858
GFF3329
Position (kb)
153
154
155 Strain fitness (log2 ratio)
-2
-1
0
1 at 152.886 kb on + strand, within GFF3326 at 152.887 kb on - strand, within GFF3326 at 152.887 kb on - strand, within GFF3326 at 152.887 kb on - strand, within GFF3326 at 153.135 kb on + strand, within GFF3326 at 153.136 kb on - strand, within GFF3326 at 153.153 kb on - strand, within GFF3326 at 153.497 kb on + strand, within GFF3327 at 153.497 kb on + strand, within GFF3327 at 153.498 kb on - strand, within GFF3327 at 153.498 kb on - strand, within GFF3327 at 153.773 kb on - strand at 153.886 kb on + strand at 153.907 kb on + strand at 153.907 kb on + strand at 153.908 kb on - strand at 153.908 kb on - strand at 154.134 kb on + strand, within GFF3328 at 154.177 kb on + strand, within GFF3328 at 154.177 kb on + strand, within GFF3328 at 154.177 kb on + strand, within GFF3328 at 154.178 kb on - strand, within GFF3328 at 154.178 kb on - strand, within GFF3328 at 154.178 kb on - strand, within GFF3328 at 154.178 kb on - strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.213 kb on + strand, within GFF3328 at 154.214 kb on - strand, within GFF3328 at 154.214 kb on - strand, within GFF3328 at 154.214 kb on - strand, within GFF3328 at 154.366 kb on + strand, within GFF3328 at 154.417 kb on + strand, within GFF3328 at 154.417 kb on + strand, within GFF3328 at 154.417 kb on + strand, within GFF3328 at 154.418 kb on - strand, within GFF3328 at 154.418 kb on - strand, within GFF3328 at 154.490 kb on - strand, within GFF3328 at 154.490 kb on - strand, within GFF3328 at 154.751 kb on - strand, within GFF3328 at 154.771 kb on + strand, within GFF3328 at 154.771 kb on + strand, within GFF3328 at 154.840 kb on + strand, within GFF3328 at 154.840 kb on + strand, within GFF3328 at 154.841 kb on - strand, within GFF3328 at 154.841 kb on - strand, within GFF3328 at 154.841 kb on - strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.843 kb on + strand, within GFF3328 at 154.844 kb on - strand, within GFF3328 at 154.844 kb on - strand, within GFF3328 at 154.844 kb on - strand, within GFF3328 at 155.031 kb on - strand at 155.031 kb on - strand at 155.051 kb on + strand at 155.051 kb on + strand at 155.052 kb on - strand at 155.052 kb on - strand at 155.213 kb on + strand at 155.380 kb on + strand, within GFF3329 at 155.380 kb on + strand, within GFF3329 at 155.446 kb on + strand, within GFF3329 at 155.447 kb on - strand, within GFF3329 at 155.546 kb on + strand, within GFF3329 at 155.903 kb on + strand, within GFF3329 at 155.903 kb on + strand, within GFF3329 at 155.904 kb on - strand, within GFF3329 at 155.904 kb on - strand, within GFF3329 at 155.921 kb on + strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329 at 155.922 kb on - strand, within GFF3329
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 152,886 + GFF3326 0.27 +0.5 152,887 - GFF3326 0.27 +0.3 152,887 - GFF3326 0.27 -0.2 152,887 - GFF3326 0.27 -0.5 153,135 + GFF3326 0.81 -0.2 153,136 - GFF3326 0.81 -0.9 153,153 - GFF3326 0.85 +0.0 153,497 + GFF3327 0.57 +0.9 153,497 + GFF3327 0.57 -0.7 153,498 - GFF3327 0.57 -0.1 153,498 - GFF3327 0.57 -1.0 153,773 - +1.3 153,886 + -0.1 153,907 + -0.7 153,907 + -0.2 153,908 - +0.2 153,908 - -0.4 154,134 + GFF3328 0.25 +0.9 154,177 + GFF3328 0.29 +0.2 154,177 + GFF3328 0.29 -0.1 154,177 + GFF3328 0.29 -0.8 154,178 - GFF3328 0.29 +0.9 154,178 - GFF3328 0.29 -0.1 154,178 - GFF3328 0.29 +1.3 154,178 - GFF3328 0.29 +0.1 154,213 + GFF3328 0.32 -0.1 154,213 + GFF3328 0.32 -1.5 154,213 + GFF3328 0.32 +0.6 154,213 + GFF3328 0.32 +0.8 154,213 + GFF3328 0.32 -0.4 154,213 + GFF3328 0.32 -0.1 154,214 - GFF3328 0.32 +0.1 154,214 - GFF3328 0.32 +0.2 154,214 - GFF3328 0.32 -0.8 154,366 + GFF3328 0.46 +1.1 154,417 + GFF3328 0.50 -0.5 154,417 + GFF3328 0.50 -0.7 154,417 + GFF3328 0.50 -0.0 154,418 - GFF3328 0.50 +0.3 154,418 - GFF3328 0.50 +0.6 154,490 - GFF3328 0.57 -0.6 154,490 - GFF3328 0.57 -0.8 154,751 - GFF3328 0.81 -1.8 154,771 + GFF3328 0.82 +0.9 154,771 + GFF3328 0.82 -0.0 154,840 + GFF3328 0.89 -0.9 154,840 + GFF3328 0.89 -0.3 154,841 - GFF3328 0.89 +0.9 154,841 - GFF3328 0.89 -0.5 154,841 - GFF3328 0.89 -1.5 154,843 + GFF3328 0.89 +0.3 154,843 + GFF3328 0.89 +0.9 154,843 + GFF3328 0.89 +0.2 154,843 + GFF3328 0.89 -0.2 154,843 + GFF3328 0.89 +0.3 154,843 + GFF3328 0.89 +0.5 154,843 + GFF3328 0.89 +0.1 154,843 + GFF3328 0.89 -0.1 154,843 + GFF3328 0.89 +1.5 154,843 + GFF3328 0.89 -0.1 154,844 - GFF3328 0.89 +0.0 154,844 - GFF3328 0.89 -1.1 154,844 - GFF3328 0.89 -0.2 155,031 - -0.6 155,031 - +0.3 155,051 + -0.9 155,051 + +1.2 155,052 - +0.5 155,052 - +0.3 155,213 + -0.1 155,380 + GFF3329 0.18 +0.5 155,380 + GFF3329 0.18 -0.3 155,446 + GFF3329 0.22 +0.3 155,447 - GFF3329 0.22 -0.6 155,546 + GFF3329 0.27 -1.8 155,903 + GFF3329 0.47 +1.3 155,903 + GFF3329 0.47 -0.2 155,904 - GFF3329 0.47 -0.0 155,904 - GFF3329 0.47 -0.1 155,921 + GFF3329 0.48 -0.3 155,922 - GFF3329 0.48 +0.0 155,922 - GFF3329 0.48 -0.2 155,922 - GFF3329 0.48 +1.4 155,922 - GFF3329 0.48 -0.0 155,922 - GFF3329 0.48 -0.9 155,922 - GFF3329 0.48 +0.0 155,922 - GFF3329 0.48 -0.3
Or see this region's nucleotide sequence