Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3233 and GFF3234 are separated by 1 nucleotides GFF3234 and GFF3235 are separated by 55 nucleotides GFF3235 and GFF3236 overlap by 1 nucleotides GFF3236 and GFF3237 overlap by 4 nucleotides GFF3237 and GFF3238 overlap by 4 nucleotides GFF3238 and GFF3239 overlap by 4 nucleotides GFF3239 and GFF3240 are separated by 11 nucleotides
GFF3233 - hypothetical protein, at 51,875 to 52,627
GFF3233
GFF3234 - no description, at 52,629 to 52,748
GFF3234
GFF3235 - no description, at 52,804 to 53,175
GFF3235
GFF3236 - no description, at 53,175 to 53,573
GFF3236
GFF3237 - no description, at 53,570 to 53,809
GFF3237
GFF3238 - no description, at 53,806 to 54,216
GFF3238
GFF3239 - no description, at 54,213 to 54,605
GFF3239
GFF3240 - hypothetical protein, at 54,617 to 55,237
GFF3240
Position (kb)
53
54 Strain fitness (log2 ratio)
-1
0
1
2 at 52.789 kb on - strand at 52.821 kb on - strand at 52.949 kb on - strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.115 kb on + strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.116 kb on - strand, within GFF3235 at 53.203 kb on + strand at 53.203 kb on + strand at 53.204 kb on - strand at 53.204 kb on - strand at 53.514 kb on + strand, within GFF3236 at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.591 kb on + strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.592 kb on - strand at 53.717 kb on + strand, within GFF3237 at 53.958 kb on + strand, within GFF3238 at 53.959 kb on - strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.109 kb on + strand, within GFF3238 at 54.110 kb on - strand, within GFF3238 at 54.110 kb on - strand, within GFF3238 at 54.196 kb on + strand at 54.197 kb on - strand at 54.426 kb on + strand, within GFF3239 at 54.426 kb on + strand, within GFF3239 at 54.427 kb on - strand, within GFF3239 at 54.429 kb on + strand, within GFF3239 at 54.472 kb on - strand, within GFF3239 at 54.472 kb on - strand, within GFF3239
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 52,789 - -1.3 52,821 - -0.4 52,949 - GFF3235 0.39 -0.7 53,115 + GFF3235 0.84 -0.6 53,115 + GFF3235 0.84 +0.2 53,115 + GFF3235 0.84 -0.4 53,115 + GFF3235 0.84 +0.2 53,115 + GFF3235 0.84 +0.8 53,115 + GFF3235 0.84 -0.8 53,116 - GFF3235 0.84 -0.4 53,116 - GFF3235 0.84 +0.4 53,116 - GFF3235 0.84 -0.1 53,116 - GFF3235 0.84 -0.9 53,116 - GFF3235 0.84 +0.1 53,116 - GFF3235 0.84 +0.7 53,116 - GFF3235 0.84 -0.2 53,203 + -0.3 53,203 + +1.0 53,204 - +0.9 53,204 - -0.3 53,514 + GFF3236 0.85 -0.2 53,591 + -0.3 53,591 + +0.1 53,591 + -0.1 53,591 + +0.2 53,591 + +2.5 53,592 - -0.8 53,592 - -0.1 53,592 - -1.0 53,592 - +0.2 53,592 - +0.3 53,717 + GFF3237 0.61 +0.2 53,958 + GFF3238 0.37 +0.7 53,959 - GFF3238 0.37 +0.7 54,109 + GFF3238 0.74 +0.5 54,109 + GFF3238 0.74 -0.7 54,109 + GFF3238 0.74 -0.9 54,109 + GFF3238 0.74 +0.1 54,110 - GFF3238 0.74 +0.2 54,110 - GFF3238 0.74 +0.3 54,196 + +0.2 54,197 - -0.4 54,426 + GFF3239 0.54 -0.9 54,426 + GFF3239 0.54 +0.0 54,427 - GFF3239 0.54 -0.4 54,429 + GFF3239 0.55 -1.5 54,472 - GFF3239 0.66 -0.8 54,472 - GFF3239 0.66 -0.0
Or see this region's nucleotide sequence