Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF316 and GFF317 are separated by 5 nucleotides GFF317 and GFF318 are separated by 98 nucleotides GFF318 and GFF319 are separated by 17 nucleotides GFF319 and GFF320 overlap by 4 nucleotides
GFF316 - Transmembrane protein, at 349,904 to 350,548
GFF316
GFF317 - Acyl carrier protein, at 350,554 to 350,865
GFF317
GFF318 - Uncharacterized protein YjgR, at 350,964 to 352,481
GFF318
GFF319 - EXTRACYTOPLASMIC FUNCTION ALTERNATIVE SIGMA FACTOR, at 352,499 to 353,140
GFF319
GFF320 - RNA polymerase ECF-type sigma factor, at 353,137 to 353,727
GFF320
Position (kb)
350
351
352
353 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.975 kb on + strand, within GFF316 at 349.976 kb on - strand, within GFF316 at 349.976 kb on - strand, within GFF316 at 349.976 kb on - strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.097 kb on + strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.098 kb on - strand, within GFF316 at 350.133 kb on + strand, within GFF316 at 350.133 kb on + strand, within GFF316 at 350.134 kb on - strand, within GFF316 at 350.134 kb on - strand, within GFF316 at 350.134 kb on - strand, within GFF316 at 350.134 kb on - strand, within GFF316 at 350.134 kb on - strand, within GFF316 at 350.166 kb on + strand, within GFF316 at 350.167 kb on - strand, within GFF316 at 350.167 kb on - strand, within GFF316 at 350.167 kb on - strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.298 kb on + strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.299 kb on - strand, within GFF316 at 350.502 kb on + strand at 350.502 kb on + strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.503 kb on - strand at 350.559 kb on + strand at 350.560 kb on - strand at 350.560 kb on - strand at 350.720 kb on + strand, within GFF317 at 350.721 kb on - strand, within GFF317 at 350.721 kb on - strand, within GFF317 at 350.851 kb on + strand at 350.851 kb on + strand at 350.851 kb on + strand at 350.852 kb on - strand at 350.852 kb on - strand at 350.880 kb on + strand at 350.880 kb on + strand at 350.880 kb on + strand at 350.880 kb on + strand at 350.881 kb on - strand at 350.881 kb on - strand at 350.881 kb on - strand at 350.894 kb on - strand at 350.914 kb on + strand at 350.914 kb on + strand at 350.915 kb on - strand at 350.915 kb on - strand at 350.915 kb on - strand at 350.931 kb on + strand at 350.931 kb on + strand at 350.932 kb on - strand at 350.946 kb on + strand at 350.946 kb on + strand at 350.946 kb on + strand at 350.946 kb on + strand at 350.946 kb on + strand at 350.946 kb on + strand at 350.947 kb on - strand at 350.947 kb on - strand at 350.947 kb on - strand at 350.963 kb on + strand at 350.963 kb on + strand at 350.963 kb on + strand at 350.963 kb on + strand at 350.963 kb on + strand at 350.964 kb on - strand at 350.964 kb on - strand at 351.403 kb on + strand, within GFF318 at 351.403 kb on + strand, within GFF318 at 351.403 kb on + strand, within GFF318 at 351.403 kb on + strand, within GFF318 at 351.404 kb on - strand, within GFF318 at 351.404 kb on - strand, within GFF318 at 351.404 kb on - strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.441 kb on + strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.442 kb on - strand, within GFF318 at 351.663 kb on + strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.664 kb on - strand, within GFF318 at 351.852 kb on + strand, within GFF318 at 351.852 kb on + strand, within GFF318 at 351.852 kb on + strand, within GFF318 at 351.852 kb on + strand, within GFF318 at 351.853 kb on - strand, within GFF318 at 352.212 kb on + strand, within GFF318 at 352.213 kb on - strand, within GFF318 at 352.213 kb on - strand, within GFF318 at 352.213 kb on - strand, within GFF318 at 352.340 kb on + strand at 352.500 kb on + strand at 352.500 kb on + strand at 352.500 kb on + strand at 352.501 kb on - strand at 352.566 kb on + strand, within GFF319 at 352.566 kb on + strand, within GFF319 at 352.567 kb on - strand, within GFF319 at 352.567 kb on - strand, within GFF319 at 352.567 kb on - strand, within GFF319 at 352.567 kb on - strand, within GFF319 at 352.567 kb on - strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.614 kb on + strand, within GFF319 at 352.615 kb on - strand, within GFF319 at 352.615 kb on - strand, within GFF319 at 353.007 kb on + strand, within GFF319 at 353.008 kb on - strand, within GFF319 at 353.008 kb on - strand, within GFF319 at 353.008 kb on - strand, within GFF319 at 353.283 kb on + strand, within GFF320 at 353.283 kb on + strand, within GFF320 at 353.283 kb on + strand, within GFF320 at 353.284 kb on - strand, within GFF320 at 353.284 kb on - strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.447 kb on + strand, within GFF320 at 353.448 kb on - strand, within GFF320 at 353.448 kb on - strand, within GFF320
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 349,975 + GFF316 0.11 +1.3 349,975 + GFF316 0.11 +0.5 349,975 + GFF316 0.11 -0.1 349,975 + GFF316 0.11 -0.0 349,975 + GFF316 0.11 -2.1 349,975 + GFF316 0.11 -0.3 349,975 + GFF316 0.11 +0.4 349,976 - GFF316 0.11 -1.5 349,976 - GFF316 0.11 -2.1 349,976 - GFF316 0.11 +0.2 350,097 + GFF316 0.30 +0.1 350,097 + GFF316 0.30 +0.3 350,097 + GFF316 0.30 +0.3 350,097 + GFF316 0.30 +0.5 350,097 + GFF316 0.30 +0.3 350,097 + GFF316 0.30 -0.1 350,097 + GFF316 0.30 -0.1 350,097 + GFF316 0.30 +0.4 350,098 - GFF316 0.30 +0.4 350,098 - GFF316 0.30 +0.7 350,098 - GFF316 0.30 -0.7 350,098 - GFF316 0.30 +0.5 350,098 - GFF316 0.30 +0.7 350,098 - GFF316 0.30 +0.5 350,098 - GFF316 0.30 +0.3 350,133 + GFF316 0.36 -0.3 350,133 + GFF316 0.36 +1.8 350,134 - GFF316 0.36 -0.5 350,134 - GFF316 0.36 +1.6 350,134 - GFF316 0.36 +0.4 350,134 - GFF316 0.36 +1.3 350,134 - GFF316 0.36 -0.0 350,166 + GFF316 0.41 -0.1 350,167 - GFF316 0.41 +0.9 350,167 - GFF316 0.41 +2.6 350,167 - GFF316 0.41 -0.0 350,298 + GFF316 0.61 -0.5 350,298 + GFF316 0.61 -0.9 350,298 + GFF316 0.61 +1.5 350,298 + GFF316 0.61 -1.1 350,298 + GFF316 0.61 -0.7 350,298 + GFF316 0.61 -0.3 350,298 + GFF316 0.61 +0.9 350,298 + GFF316 0.61 -0.4 350,298 + GFF316 0.61 -0.4 350,298 + GFF316 0.61 -0.1 350,298 + GFF316 0.61 -0.1 350,298 + GFF316 0.61 +0.4 350,299 - GFF316 0.61 -1.3 350,299 - GFF316 0.61 -0.5 350,299 - GFF316 0.61 -1.3 350,299 - GFF316 0.61 -1.3 350,299 - GFF316 0.61 +0.3 350,299 - GFF316 0.61 -0.4 350,299 - GFF316 0.61 -0.1 350,299 - GFF316 0.61 +0.6 350,299 - GFF316 0.61 +0.4 350,299 - GFF316 0.61 +0.7 350,299 - GFF316 0.61 +0.4 350,299 - GFF316 0.61 -1.0 350,299 - GFF316 0.61 +0.5 350,502 + -1.9 350,502 + -0.1 350,503 - +0.8 350,503 - -1.1 350,503 - -0.7 350,503 - +0.3 350,503 - -0.1 350,503 - -0.2 350,503 - -0.4 350,503 - -0.4 350,559 + -0.2 350,560 - +0.3 350,560 - +0.0 350,720 + GFF317 0.53 +0.0 350,721 - GFF317 0.54 +0.0 350,721 - GFF317 0.54 -1.9 350,851 + -1.1 350,851 + -1.0 350,851 + -0.1 350,852 - +0.8 350,852 - -0.9 350,880 + -0.1 350,880 + -0.2 350,880 + -0.7 350,880 + -1.2 350,881 - -0.0 350,881 - -2.1 350,881 - -0.4 350,894 - +0.1 350,914 + -0.0 350,914 + -0.1 350,915 - -1.5 350,915 - -0.3 350,915 - +0.0 350,931 + +1.0 350,931 + +1.3 350,932 - -0.8 350,946 + -0.4 350,946 + -0.5 350,946 + +0.5 350,946 + -0.4 350,946 + -1.5 350,946 + -0.5 350,947 - -0.4 350,947 - +0.3 350,947 - -0.4 350,963 + +0.7 350,963 + -0.3 350,963 + -1.0 350,963 + +0.4 350,963 + -0.1 350,964 - +0.1 350,964 - +1.0 351,403 + GFF318 0.29 -0.1 351,403 + GFF318 0.29 +1.3 351,403 + GFF318 0.29 -1.0 351,403 + GFF318 0.29 -1.1 351,404 - GFF318 0.29 +0.1 351,404 - GFF318 0.29 +0.3 351,404 - GFF318 0.29 -0.8 351,441 + GFF318 0.31 -0.7 351,441 + GFF318 0.31 -0.4 351,441 + GFF318 0.31 -1.0 351,441 + GFF318 0.31 +0.0 351,441 + GFF318 0.31 -0.6 351,441 + GFF318 0.31 +0.1 351,441 + GFF318 0.31 +0.1 351,442 - GFF318 0.31 -0.3 351,442 - GFF318 0.31 -0.4 351,442 - GFF318 0.31 -1.5 351,442 - GFF318 0.31 +0.3 351,442 - GFF318 0.31 -0.6 351,442 - GFF318 0.31 +0.4 351,442 - GFF318 0.31 -0.1 351,663 + GFF318 0.46 -0.3 351,664 - GFF318 0.46 -1.3 351,664 - GFF318 0.46 -1.6 351,664 - GFF318 0.46 -1.9 351,664 - GFF318 0.46 +0.3 351,664 - GFF318 0.46 -0.6 351,664 - GFF318 0.46 -0.7 351,852 + GFF318 0.58 -0.2 351,852 + GFF318 0.58 +0.1 351,852 + GFF318 0.58 +0.1 351,852 + GFF318 0.58 +0.7 351,853 - GFF318 0.59 -0.7 352,212 + GFF318 0.82 +0.4 352,213 - GFF318 0.82 -0.1 352,213 - GFF318 0.82 -0.5 352,213 - GFF318 0.82 -0.1 352,340 + -0.0 352,500 + -1.3 352,500 + -0.1 352,500 + +0.2 352,501 - -0.9 352,566 + GFF319 0.10 -0.3 352,566 + GFF319 0.10 -0.5 352,567 - GFF319 0.11 +0.3 352,567 - GFF319 0.11 -0.2 352,567 - GFF319 0.11 +0.3 352,567 - GFF319 0.11 -0.2 352,567 - GFF319 0.11 +0.3 352,614 + GFF319 0.18 -1.1 352,614 + GFF319 0.18 +0.3 352,614 + GFF319 0.18 -0.7 352,614 + GFF319 0.18 -2.1 352,614 + GFF319 0.18 +0.3 352,614 + GFF319 0.18 +0.5 352,614 + GFF319 0.18 -0.7 352,614 + GFF319 0.18 +0.1 352,615 - GFF319 0.18 -0.3 352,615 - GFF319 0.18 -1.6 353,007 + GFF319 0.79 +0.1 353,008 - GFF319 0.79 +0.7 353,008 - GFF319 0.79 +0.9 353,008 - GFF319 0.79 +0.8 353,283 + GFF320 0.25 -1.5 353,283 + GFF320 0.25 -0.7 353,283 + GFF320 0.25 -0.8 353,284 - GFF320 0.25 -0.1 353,284 - GFF320 0.25 -0.2 353,447 + GFF320 0.52 -1.1 353,447 + GFF320 0.52 -0.3 353,447 + GFF320 0.52 +0.3 353,448 - GFF320 0.53 +1.3 353,448 - GFF320 0.53 -0.6
Or see this region's nucleotide sequence