Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3025 and GFF3026 overlap by 4 nucleotides GFF3026 and GFF3027 are separated by 20 nucleotides GFF3027 and GFF3028 are separated by 5 nucleotides GFF3028 and GFF3029 are separated by 58 nucleotides
GFF3025 - Proton/sodium-glutamate symport protein, at 453,233 to 454,702
GFF3025
GFF3026 - KEGG: type VI secretion system protein, at 454,699 to 455,172
GFF3026
GFF3027 - T6SS component TssK (ImpJ/VasE), at 455,193 to 456,515
GFF3027
GFF3028 - T6SS outer membrane component TssL (ImpK/VasF), at 456,521 to 457,282
GFF3028
GFF3029 - T6SS component TssM (IcmF/VasK), at 457,341 to 461,426
GFF3029
Position (kb) 455
456
457 Strain fitness (log2 ratio) -1
0
1 at 454.196 kb on + strand, within GFF3025 at 454.196 kb on + strand, within GFF3025 at 454.197 kb on - strand, within GFF3025 at 454.197 kb on - strand, within GFF3025 at 454.199 kb on + strand, within GFF3025 at 454.200 kb on - strand, within GFF3025 at 454.232 kb on + strand, within GFF3025 at 454.233 kb on - strand, within GFF3025 at 454.662 kb on - strand at 454.976 kb on - strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.071 kb on + strand, within GFF3026 at 455.072 kb on - strand, within GFF3026 at 455.072 kb on - strand, within GFF3026 at 455.518 kb on - strand, within GFF3027 at 455.518 kb on - strand, within GFF3027 at 455.988 kb on + strand, within GFF3027 at 455.989 kb on - strand, within GFF3027 at 455.989 kb on - strand, within GFF3027 at 455.989 kb on - strand, within GFF3027 at 456.064 kb on - strand, within GFF3027 at 456.064 kb on - strand, within GFF3027 at 456.088 kb on + strand, within GFF3027 at 456.089 kb on - strand, within GFF3027 at 456.089 kb on - strand, within GFF3027 at 456.144 kb on + strand, within GFF3027 at 456.394 kb on - strand at 456.584 kb on + strand at 456.755 kb on + strand, within GFF3028 at 456.755 kb on + strand, within GFF3028 at 456.756 kb on - strand, within GFF3028 at 456.941 kb on + strand, within GFF3028 at 457.094 kb on + strand, within GFF3028 at 457.094 kb on + strand, within GFF3028 at 457.419 kb on + strand at 457.420 kb on - strand at 457.420 kb on - strand at 457.420 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 454,196 + GFF3025 0.66 +0.5 454,196 + GFF3025 0.66 -0.1 454,197 - GFF3025 0.66 -0.4 454,197 - GFF3025 0.66 -0.5 454,199 + GFF3025 0.66 -1.5 454,200 - GFF3025 0.66 -0.5 454,232 + GFF3025 0.68 +0.8 454,233 - GFF3025 0.68 +0.8 454,662 - +0.5 454,976 - GFF3026 0.58 -0.4 455,071 + GFF3026 0.78 +1.2 455,071 + GFF3026 0.78 -0.4 455,071 + GFF3026 0.78 -0.7 455,072 - GFF3026 0.79 -1.4 455,072 - GFF3026 0.79 +0.0 455,518 - GFF3027 0.25 -0.6 455,518 - GFF3027 0.25 -0.1 455,988 + GFF3027 0.60 -0.5 455,989 - GFF3027 0.60 +0.2 455,989 - GFF3027 0.60 -1.7 455,989 - GFF3027 0.60 -0.3 456,064 - GFF3027 0.66 -0.3 456,064 - GFF3027 0.66 -0.0 456,088 + GFF3027 0.68 -1.3 456,089 - GFF3027 0.68 -0.5 456,089 - GFF3027 0.68 +0.8 456,144 + GFF3027 0.72 -0.8 456,394 - -0.8 456,584 + -0.2 456,755 + GFF3028 0.31 -0.5 456,755 + GFF3028 0.31 -1.2 456,756 - GFF3028 0.31 +1.2 456,941 + GFF3028 0.55 +0.1 457,094 + GFF3028 0.75 +0.2 457,094 + GFF3028 0.75 -1.7 457,419 + -0.0 457,420 - -0.3 457,420 - +0.1 457,420 - -1.1
Or see this region's nucleotide sequence