Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3018 and GFF3019 are separated by 53 nucleotides GFF3019 and GFF3020 are separated by 3 nucleotides GFF3020 and GFF3021 are separated by 63 nucleotides GFF3021 and GFF3022 overlap by 4 nucleotides
GFF3018 - VgrG protein, at 446,843 to 448,783
GFF3018
GFF3019 - hypothetical protein, at 448,837 to 449,511
GFF3019
GFF3020 - FIG00966904: hypothetical protein, at 449,515 to 449,904
GFF3020
GFF3021 - no description, at 449,968 to 450,150
GFF3021
GFF3022 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 450,147 to 451,199
GFF3022
Position (kb) 448
449
450 Strain fitness (log2 ratio) -2
-1
0
1
2 at 448.109 kb on + strand, within GFF3018 at 448.109 kb on + strand, within GFF3018 at 448.109 kb on + strand, within GFF3018 at 448.109 kb on + strand, within GFF3018 at 448.109 kb on + strand, within GFF3018 at 448.110 kb on - strand, within GFF3018 at 448.110 kb on - strand, within GFF3018 at 448.110 kb on - strand, within GFF3018 at 448.110 kb on - strand, within GFF3018 at 448.154 kb on + strand, within GFF3018 at 448.289 kb on + strand, within GFF3018 at 448.289 kb on + strand, within GFF3018 at 448.289 kb on + strand, within GFF3018 at 448.289 kb on + strand, within GFF3018 at 448.290 kb on - strand, within GFF3018 at 448.290 kb on - strand, within GFF3018 at 448.511 kb on + strand, within GFF3018 at 448.511 kb on + strand, within GFF3018 at 448.512 kb on - strand, within GFF3018 at 448.960 kb on + strand, within GFF3019 at 449.069 kb on - strand, within GFF3019 at 449.072 kb on - strand, within GFF3019 at 449.176 kb on + strand, within GFF3019 at 449.176 kb on + strand, within GFF3019 at 449.209 kb on + strand, within GFF3019 at 449.210 kb on - strand, within GFF3019 at 449.210 kb on - strand, within GFF3019 at 449.210 kb on - strand, within GFF3019 at 449.210 kb on - strand, within GFF3019 at 449.210 kb on - strand, within GFF3019 at 449.282 kb on - strand, within GFF3019 at 449.317 kb on + strand, within GFF3019 at 449.317 kb on + strand, within GFF3019 at 449.317 kb on + strand, within GFF3019 at 449.318 kb on - strand, within GFF3019 at 449.944 kb on + strand at 449.944 kb on + strand at 449.945 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 448,109 + GFF3018 0.65 +0.1 448,109 + GFF3018 0.65 -0.3 448,109 + GFF3018 0.65 +0.3 448,109 + GFF3018 0.65 +0.5 448,109 + GFF3018 0.65 -0.0 448,110 - GFF3018 0.65 -1.9 448,110 - GFF3018 0.65 +0.1 448,110 - GFF3018 0.65 -0.3 448,110 - GFF3018 0.65 -0.5 448,154 + GFF3018 0.68 -2.7 448,289 + GFF3018 0.74 -2.5 448,289 + GFF3018 0.74 -0.2 448,289 + GFF3018 0.74 -0.6 448,289 + GFF3018 0.74 -0.2 448,290 - GFF3018 0.75 -1.3 448,290 - GFF3018 0.75 +0.5 448,511 + GFF3018 0.86 +0.0 448,511 + GFF3018 0.86 -0.4 448,512 - GFF3018 0.86 +0.1 448,960 + GFF3019 0.18 +0.4 449,069 - GFF3019 0.34 -0.6 449,072 - GFF3019 0.35 +0.0 449,176 + GFF3019 0.50 +0.3 449,176 + GFF3019 0.50 -1.0 449,209 + GFF3019 0.55 -0.8 449,210 - GFF3019 0.55 -0.9 449,210 - GFF3019 0.55 -1.7 449,210 - GFF3019 0.55 -0.7 449,210 - GFF3019 0.55 -0.4 449,210 - GFF3019 0.55 -1.5 449,282 - GFF3019 0.66 +0.8 449,317 + GFF3019 0.71 +1.8 449,317 + GFF3019 0.71 +0.1 449,317 + GFF3019 0.71 -0.9 449,318 - GFF3019 0.71 +0.4 449,944 + +0.4 449,944 + -0.8 449,945 - -0.8
Or see this region's nucleotide sequence