Strain Fitness in Variovorax sp. SCN45 around GFF3006

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

Add experiment(s):

Zoom: Pan:

Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

download SVG
500 ntGFF3004 and GFF3005 are separated by 5 nucleotidesGFF3005 and GFF3006 overlap by 4 nucleotidesGFF3006 and GFF3007 are separated by 165 nucleotidesGFF3007 and GFF3008 overlap by 14 nucleotidesGFF3008 and GFF3009 are separated by 16 nucleotides GFF3004 - Thymidylate synthase (EC 2.1.1.45), at 432,959 to 433,828 GFF3004 GFF3005 - Dihydrofolate reductase (EC 1.5.1.3), at 433,834 to 434,355 GFF3005 GFF3006 - hypothetical protein, at 434,352 to 435,473 GFF3006 GFF3007 - hypothetical protein, at 435,639 to 435,878 GFF3007 GFF3008 - hypothetical protein, at 435,865 to 436,215 GFF3008 GFF3009 - NADP-specific glutamate dehydrogenase (EC 1.4.1.4), at 436,232 to 437,575 GFF3009 Position (kb) 434 435 436Strain fitness (log2 ratio) -2 -1 0 1 2at 434.610 kb on + strand, within GFF3006at 434.611 kb on - strand, within GFF3006at 434.611 kb on - strand, within GFF3006at 435.063 kb on + strand, within GFF3006at 435.063 kb on + strand, within GFF3006at 435.064 kb on - strand, within GFF3006at 435.064 kb on - strand, within GFF3006at 435.082 kb on + strand, within GFF3006at 435.082 kb on + strand, within GFF3006at 435.082 kb on + strand, within GFF3006at 435.082 kb on + strand, within GFF3006at 435.083 kb on - strand, within GFF3006at 435.083 kb on - strand, within GFF3006at 435.083 kb on - strand, within GFF3006at 435.083 kb on - strand, within GFF3006at 435.090 kb on + strand, within GFF3006at 435.297 kb on + strand, within GFF3006at 435.297 kb on + strand, within GFF3006at 435.297 kb on + strand, within GFF3006at 435.297 kb on + strand, within GFF3006at 435.298 kb on - strand, within GFF3006at 435.298 kb on - strand, within GFF3006at 435.298 kb on - strand, within GFF3006at 435.384 kb on + strandat 435.539 kb on + strandat 435.540 kb on - strandat 435.574 kb on - strandat 435.583 kb on + strandat 435.583 kb on + strandat 435.583 kb on + strandat 435.583 kb on + strandat 435.584 kb on - strandat 435.584 kb on - strandat 435.584 kb on - strandat 435.584 kb on - strandat 435.584 kb on - strandat 435.584 kb on - strandat 435.649 kb on - strandat 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.684 kb on + strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.685 kb on - strand, within GFF3007at 435.746 kb on - strand, within GFF3007at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.986 kb on + strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 435.987 kb on - strand, within GFF3008at 436.092 kb on - strand, within GFF3008at 436.092 kb on - strand, within GFF3008at 436.092 kb on - strand, within GFF3008at 436.129 kb on + strand, within GFF3008at 436.130 kb on + strand, within GFF3008at 436.130 kb on + strand, within GFF3008at 436.130 kb on + strand, within GFF3008at 436.131 kb on - strand, within GFF3008at 436.131 kb on - strand, within GFF3008at 436.228 kb on - strandat 436.302 kb on + strandat 436.302 kb on + strandat 436.302 kb on + strandat 436.302 kb on + strandat 436.302 kb on + strandat 436.302 kb on + strand

download strain data

Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
remove
434,610 + GFF3006 0.23 -0.0
434,611 - GFF3006 0.23 -0.6
434,611 - GFF3006 0.23 +0.1
435,063 + GFF3006 0.63 -0.2
435,063 + GFF3006 0.63 -0.2
435,064 - GFF3006 0.63 +0.6
435,064 - GFF3006 0.63 -0.7
435,082 + GFF3006 0.65 -1.0
435,082 + GFF3006 0.65 -0.0
435,082 + GFF3006 0.65 -0.3
435,082 + GFF3006 0.65 -1.8
435,083 - GFF3006 0.65 +0.4
435,083 - GFF3006 0.65 -0.6
435,083 - GFF3006 0.65 -0.2
435,083 - GFF3006 0.65 -0.9
435,090 + GFF3006 0.66 -2.0
435,297 + GFF3006 0.84 -0.5
435,297 + GFF3006 0.84 -0.5
435,297 + GFF3006 0.84 -0.3
435,297 + GFF3006 0.84 -0.7
435,298 - GFF3006 0.84 -0.4
435,298 - GFF3006 0.84 +0.5
435,298 - GFF3006 0.84 -1.9
435,384 + +0.3
435,539 + -0.7
435,540 - -0.7
435,574 - -0.7
435,583 + -2.0
435,583 + -0.6
435,583 + -0.0
435,583 + -0.1
435,584 - -1.8
435,584 - +0.9
435,584 - -0.3
435,584 - +0.5
435,584 - +0.5
435,584 - +0.3
435,649 - +0.5
435,684 + GFF3007 0.19 +1.0
435,684 + GFF3007 0.19 -0.4
435,684 + GFF3007 0.19 -0.3
435,684 + GFF3007 0.19 -0.1
435,684 + GFF3007 0.19 +1.0
435,684 + GFF3007 0.19 -0.1
435,684 + GFF3007 0.19 -1.2
435,684 + GFF3007 0.19 +0.1
435,684 + GFF3007 0.19 -0.5
435,684 + GFF3007 0.19 -0.7
435,685 - GFF3007 0.19 +1.5
435,685 - GFF3007 0.19 -0.9
435,685 - GFF3007 0.19 +0.8
435,685 - GFF3007 0.19 -2.5
435,685 - GFF3007 0.19 +0.5
435,685 - GFF3007 0.19 +0.0
435,685 - GFF3007 0.19 +0.1
435,685 - GFF3007 0.19 -0.9
435,746 - GFF3007 0.45 +1.9
435,986 + GFF3008 0.34 +0.7
435,986 + GFF3008 0.34 +1.3
435,986 + GFF3008 0.34 -2.2
435,986 + GFF3008 0.34 +0.7
435,986 + GFF3008 0.34 +0.2
435,986 + GFF3008 0.34 -1.5
435,986 + GFF3008 0.34 +1.0
435,986 + GFF3008 0.34 +0.7
435,986 + GFF3008 0.34 -0.3
435,986 + GFF3008 0.34 -0.8
435,986 + GFF3008 0.34 -0.3
435,986 + GFF3008 0.34 -0.2
435,987 - GFF3008 0.35 +0.4
435,987 - GFF3008 0.35 +0.1
435,987 - GFF3008 0.35 -0.8
435,987 - GFF3008 0.35 -0.6
435,987 - GFF3008 0.35 +0.0
435,987 - GFF3008 0.35 -0.5
435,987 - GFF3008 0.35 -0.1
435,987 - GFF3008 0.35 -1.3
436,092 - GFF3008 0.65 +0.3
436,092 - GFF3008 0.65 -0.1
436,092 - GFF3008 0.65 -1.0
436,129 + GFF3008 0.75 -0.2
436,130 + GFF3008 0.75 -1.0
436,130 + GFF3008 0.75 +1.4
436,130 + GFF3008 0.75 +0.5
436,131 - GFF3008 0.76 -0.2
436,131 - GFF3008 0.76 -0.8
436,228 - -0.3
436,302 + -0.9
436,302 + +0.3
436,302 + -0.7
436,302 + -1.3
436,302 + -1.2
436,302 + -0.7

Or see this region's nucleotide sequence