Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2988 and GFF2989 are separated by 8 nucleotides GFF2989 and GFF2990 are separated by 50 nucleotides GFF2990 and GFF2991 are separated by 18 nucleotides
GFF2988 - O-acetylhomoserine sulfhydrylase (EC 2.5.1.49) @ O-succinylhomoserine sulfhydrylase (EC 2.5.1.48), at 418,335 to 419,639
GFF2988
GFF2989 - hypothetical protein, at 419,648 to 421,054
GFF2989
GFF2990 - Probable hydrolase protein (EC 3.-.-.-), at 421,105 to 421,926
GFF2990
GFF2991 - BUG/TctC family periplasmic protein, at 421,945 to 422,904
GFF2991
Position (kb) 419
420
421
422 Strain fitness (log2 ratio) -2
-1
0
1
2 at 418.671 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.698 kb on + strand, within GFF2988 at 418.699 kb on - strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.908 kb on + strand, within GFF2988 at 418.909 kb on - strand, within GFF2988 at 418.947 kb on + strand, within GFF2988 at 418.948 kb on - strand, within GFF2988 at 418.948 kb on - strand, within GFF2988 at 419.064 kb on + strand, within GFF2988 at 419.064 kb on + strand, within GFF2988 at 419.065 kb on - strand, within GFF2988 at 419.668 kb on - strand at 419.753 kb on + strand at 419.754 kb on - strand at 419.754 kb on - strand at 419.966 kb on + strand, within GFF2989 at 419.967 kb on - strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.029 kb on + strand, within GFF2989 at 420.030 kb on - strand, within GFF2989 at 420.134 kb on + strand, within GFF2989 at 420.134 kb on + strand, within GFF2989 at 420.201 kb on + strand, within GFF2989 at 420.230 kb on + strand, within GFF2989 at 420.231 kb on - strand, within GFF2989 at 420.589 kb on - strand, within GFF2989 at 420.674 kb on + strand, within GFF2989 at 420.674 kb on + strand, within GFF2989 at 420.707 kb on + strand, within GFF2989 at 420.707 kb on + strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.708 kb on - strand, within GFF2989 at 420.905 kb on + strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 420.906 kb on - strand, within GFF2989 at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.138 kb on + strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.139 kb on - strand at 421.144 kb on + strand at 421.144 kb on + strand at 421.144 kb on + strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.145 kb on - strand at 421.613 kb on - strand, within GFF2990 at 421.702 kb on + strand, within GFF2990 at 421.703 kb on - strand, within GFF2990 at 421.703 kb on - strand, within GFF2990
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 418,671 + GFF2988 0.26 -1.5 418,698 + GFF2988 0.28 -2.5 418,698 + GFF2988 0.28 -0.8 418,698 + GFF2988 0.28 -0.7 418,698 + GFF2988 0.28 -1.5 418,699 - GFF2988 0.28 -2.3 418,908 + GFF2988 0.44 -0.7 418,908 + GFF2988 0.44 -0.3 418,908 + GFF2988 0.44 +1.1 418,908 + GFF2988 0.44 -1.2 418,908 + GFF2988 0.44 -1.0 418,908 + GFF2988 0.44 -0.3 418,909 - GFF2988 0.44 -0.1 418,947 + GFF2988 0.47 +0.1 418,948 - GFF2988 0.47 -0.1 418,948 - GFF2988 0.47 -0.2 419,064 + GFF2988 0.56 -0.1 419,064 + GFF2988 0.56 +0.1 419,065 - GFF2988 0.56 -0.2 419,668 - -0.4 419,753 + +0.3 419,754 - -1.3 419,754 - +0.3 419,966 + GFF2989 0.23 +0.0 419,967 - GFF2989 0.23 +1.4 420,029 + GFF2989 0.27 +2.1 420,029 + GFF2989 0.27 +0.1 420,029 + GFF2989 0.27 -0.6 420,030 - GFF2989 0.27 -1.0 420,134 + GFF2989 0.35 -1.6 420,134 + GFF2989 0.35 +1.1 420,201 + GFF2989 0.39 -0.2 420,230 + GFF2989 0.41 -1.9 420,231 - GFF2989 0.41 +1.3 420,589 - GFF2989 0.67 -0.6 420,674 + GFF2989 0.73 +1.5 420,674 + GFF2989 0.73 +1.5 420,707 + GFF2989 0.75 -0.3 420,707 + GFF2989 0.75 +0.6 420,708 - GFF2989 0.75 +0.0 420,708 - GFF2989 0.75 +0.6 420,708 - GFF2989 0.75 +0.4 420,708 - GFF2989 0.75 -0.8 420,905 + GFF2989 0.89 -0.2 420,906 - GFF2989 0.89 +0.7 420,906 - GFF2989 0.89 -0.0 420,906 - GFF2989 0.89 -0.4 421,138 + +1.0 421,138 + -0.7 421,138 + +0.9 421,138 + -0.2 421,138 + +0.2 421,139 - -0.2 421,139 - -0.6 421,139 - +0.7 421,139 - +0.4 421,139 - -0.4 421,139 - -0.3 421,139 - -0.5 421,139 - -1.0 421,139 - +0.9 421,139 - -0.6 421,139 - -0.5 421,139 - -0.8 421,139 - -0.1 421,139 - -0.1 421,139 - -0.4 421,139 - +0.0 421,139 - -1.8 421,144 + -0.2 421,144 + -0.5 421,144 + -0.6 421,145 - -1.9 421,145 - -1.1 421,145 - -0.5 421,145 - +0.7 421,145 - +0.7 421,145 - -0.9 421,613 - GFF2990 0.62 -0.3 421,702 + GFF2990 0.73 +0.1 421,703 - GFF2990 0.73 -1.3 421,703 - GFF2990 0.73 -0.2
Or see this region's nucleotide sequence