Strain Fitness in Variovorax sp. SCN45 around GFF2965

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2964 and GFF2965 are separated by 15 nucleotidesGFF2965 and GFF2966 are separated by 4 nucleotidesGFF2966 and GFF2967 overlap by 8 nucleotides GFF2964 - L-carnitine dehydratase/bile acid-inducible protein F, at 395,104 to 396,366 GFF2964 GFF2965 - Acetyl-CoA acetyltransferase (EC 2.3.1.9), at 396,382 to 397,578 GFF2965 GFF2966 - Enoyl-CoA hydratase (EC 4.2.1.17), at 397,583 to 398,395 GFF2966 GFF2967 - Oxidoreductase, short-chain dehydrogenase/reductase family, at 398,388 to 399,137 GFF2967 Position (kb) 396 397 398Strain fitness (log2 ratio) -2 -1 0 1 2at 395.399 kb on + strand, within GFF2964at 395.400 kb on - strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.420 kb on + strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.421 kb on - strand, within GFF2964at 395.675 kb on + strand, within GFF2964at 395.676 kb on - strand, within GFF2964at 395.849 kb on + strand, within GFF2964at 395.850 kb on - strand, within GFF2964at 395.951 kb on + strand, within GFF2964at 396.106 kb on + strand, within GFF2964at 396.106 kb on + strand, within GFF2964at 396.107 kb on - strand, within GFF2964at 396.107 kb on - strand, within GFF2964at 396.107 kb on - strand, within GFF2964at 396.180 kb on - strand, within GFF2964at 396.180 kb on - strand, within GFF2964at 396.230 kb on + strand, within GFF2964at 396.231 kb on - strand, within GFF2964at 396.252 kb on + strandat 396.252 kb on + strandat 396.252 kb on + strandat 396.252 kb on + strandat 396.252 kb on + strandat 396.252 kb on + strandat 396.252 kb on + strandat 396.253 kb on - strandat 396.253 kb on - strandat 396.258 kb on + strandat 396.359 kb on + strandat 396.359 kb on + strandat 396.359 kb on + strandat 396.359 kb on + strandat 396.359 kb on + strandat 396.359 kb on + strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 396.360 kb on - strandat 397.062 kb on - strand, within GFF2965at 397.062 kb on - strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.208 kb on + strand, within GFF2965at 397.209 kb on - strand, within GFF2965at 397.209 kb on - strand, within GFF2965at 397.209 kb on - strand, within GFF2965at 397.209 kb on - strand, within GFF2965at 397.211 kb on + strand, within GFF2965at 397.212 kb on - strand, within GFF2965at 397.212 kb on - strand, within GFF2965at 397.212 kb on - strand, within GFF2965at 397.409 kb on + strand, within GFF2965at 397.410 kb on - strand, within GFF2965at 397.410 kb on - strand, within GFF2965at 397.410 kb on - strand, within GFF2965at 397.410 kb on - strand, within GFF2965at 397.805 kb on + strand, within GFF2966at 398.395 kb on + strandat 398.395 kb on + strandat 398.396 kb on - strandat 398.396 kb on - strandat 398.396 kb on - strandat 398.542 kb on + strand, within GFF2967at 398.542 kb on + strand, within GFF2967at 398.542 kb on + strand, within GFF2967at 398.542 kb on + strand, within GFF2967at 398.542 kb on + strand, within GFF2967at 398.543 kb on - strand, within GFF2967at 398.543 kb on - strand, within GFF2967

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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395,399 + GFF2964 0.23 -0.2
395,400 - GFF2964 0.23 -0.6
395,420 + GFF2964 0.25 -0.2
395,420 + GFF2964 0.25 -1.3
395,420 + GFF2964 0.25 -0.3
395,420 + GFF2964 0.25 -0.6
395,420 + GFF2964 0.25 -0.2
395,420 + GFF2964 0.25 -0.2
395,420 + GFF2964 0.25 -0.0
395,420 + GFF2964 0.25 -0.2
395,421 - GFF2964 0.25 +1.4
395,421 - GFF2964 0.25 +0.0
395,421 - GFF2964 0.25 -0.0
395,421 - GFF2964 0.25 -0.7
395,421 - GFF2964 0.25 -0.4
395,421 - GFF2964 0.25 -2.1
395,421 - GFF2964 0.25 +0.3
395,421 - GFF2964 0.25 +0.2
395,421 - GFF2964 0.25 +0.7
395,421 - GFF2964 0.25 -0.6
395,675 + GFF2964 0.45 +0.6
395,676 - GFF2964 0.45 -1.1
395,849 + GFF2964 0.59 +0.2
395,850 - GFF2964 0.59 -1.8
395,951 + GFF2964 0.67 -0.4
396,106 + GFF2964 0.79 +1.1
396,106 + GFF2964 0.79 -0.8
396,107 - GFF2964 0.79 -0.4
396,107 - GFF2964 0.79 -1.6
396,107 - GFF2964 0.79 -1.4
396,180 - GFF2964 0.85 -0.7
396,180 - GFF2964 0.85 -0.8
396,230 + GFF2964 0.89 -0.8
396,231 - GFF2964 0.89 -1.3
396,252 + +1.6
396,252 + +0.6
396,252 + -0.4
396,252 + +0.3
396,252 + -0.3
396,252 + -0.6
396,252 + +0.2
396,253 - +0.1
396,253 - -0.3
396,258 + +0.1
396,359 + +0.1
396,359 + -0.9
396,359 + +0.3
396,359 + -0.3
396,359 + -0.1
396,359 + +0.4
396,360 - -0.4
396,360 - +0.3
396,360 - -0.3
396,360 - +0.3
396,360 - +0.5
396,360 - +0.3
396,360 - -0.1
396,360 - -0.8
396,360 - +0.5
397,062 - GFF2965 0.57 -0.7
397,062 - GFF2965 0.57 -0.3
397,208 + GFF2965 0.69 -0.1
397,208 + GFF2965 0.69 -0.8
397,208 + GFF2965 0.69 -1.0
397,208 + GFF2965 0.69 +0.3
397,208 + GFF2965 0.69 +0.3
397,208 + GFF2965 0.69 +0.3
397,208 + GFF2965 0.69 -0.7
397,209 - GFF2965 0.69 -0.4
397,209 - GFF2965 0.69 -0.5
397,209 - GFF2965 0.69 -0.6
397,209 - GFF2965 0.69 -0.4
397,211 + GFF2965 0.69 -0.5
397,212 - GFF2965 0.69 -0.6
397,212 - GFF2965 0.69 -1.8
397,212 - GFF2965 0.69 +0.3
397,409 + GFF2965 0.86 +0.3
397,410 - GFF2965 0.86 -0.1
397,410 - GFF2965 0.86 -0.6
397,410 - GFF2965 0.86 -1.4
397,410 - GFF2965 0.86 -0.3
397,805 + GFF2966 0.27 -0.8
398,395 + -0.6
398,395 + -0.5
398,396 - +2.6
398,396 - -1.4
398,396 - -0.9
398,542 + GFF2967 0.21 +0.7
398,542 + GFF2967 0.21 +1.2
398,542 + GFF2967 0.21 -0.7
398,542 + GFF2967 0.21 -0.2
398,542 + GFF2967 0.21 -1.3
398,543 - GFF2967 0.21 +0.6
398,543 - GFF2967 0.21 -1.4

Or see this region's nucleotide sequence