Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2900 and GFF2901 overlap by 20 nucleotides GFF2901 and GFF2903 overlap by 4 nucleotides GFF2903 and GFF2902 overlap by 6 nucleotides
GFF2900 - Subtilisin-like serine proteases, at 325,486 to 327,108
GFF2900
GFF2901 - FIG00347713: hypothetical protein, at 327,089 to 328,222
GFF2901
GFF2903 - no description, at 328,219 to 328,338
GFF2903
GFF2902 - ABC transporter, substrate-binding protein (cluster 13, osmolytes) / ABC transporter, permease protein (cluster 13, osmolytes), at 328,333 to 329,859
GFF2902
Position (kb) 327
328
329 Strain fitness (log2 ratio) -2
-1
0
1 at 326.490 kb on + strand, within GFF2900 at 326.491 kb on - strand, within GFF2900 at 326.491 kb on - strand, within GFF2900 at 326.491 kb on - strand, within GFF2900 at 326.491 kb on - strand, within GFF2900 at 326.728 kb on + strand, within GFF2900 at 326.728 kb on + strand, within GFF2900 at 326.728 kb on + strand, within GFF2900 at 326.728 kb on + strand, within GFF2900 at 326.729 kb on - strand, within GFF2900 at 326.729 kb on - strand, within GFF2900 at 326.729 kb on - strand, within GFF2900 at 326.996 kb on - strand at 326.996 kb on - strand at 327.029 kb on - strand at 327.454 kb on - strand, within GFF2901 at 327.649 kb on - strand, within GFF2901 at 327.649 kb on - strand, within GFF2901 at 328.119 kb on + strand at 328.119 kb on + strand at 328.283 kb on - strand, within GFF2903 at 328.297 kb on + strand, within GFF2903 at 328.318 kb on + strand, within GFF2903 at 328.320 kb on + strand, within GFF2903 at 328.320 kb on + strand, within GFF2903 at 328.320 kb on + strand, within GFF2903 at 328.320 kb on + strand, within GFF2903 at 328.320 kb on + strand, within GFF2903 at 328.321 kb on - strand, within GFF2903 at 328.321 kb on - strand, within GFF2903 at 328.459 kb on + strand at 328.459 kb on + strand at 328.459 kb on + strand at 328.459 kb on + strand at 328.459 kb on + strand at 328.460 kb on - strand at 328.552 kb on + strand, within GFF2902 at 328.552 kb on + strand, within GFF2902 at 328.552 kb on + strand, within GFF2902 at 328.553 kb on - strand, within GFF2902 at 328.553 kb on - strand, within GFF2902 at 328.585 kb on + strand, within GFF2902 at 328.594 kb on + strand, within GFF2902 at 328.717 kb on + strand, within GFF2902 at 328.717 kb on + strand, within GFF2902 at 328.907 kb on - strand, within GFF2902 at 328.907 kb on - strand, within GFF2902 at 328.941 kb on - strand, within GFF2902 at 328.948 kb on + strand, within GFF2902 at 328.948 kb on + strand, within GFF2902 at 328.948 kb on + strand, within GFF2902 at 328.948 kb on + strand, within GFF2902 at 328.948 kb on + strand, within GFF2902 at 328.949 kb on - strand, within GFF2902 at 328.949 kb on - strand, within GFF2902 at 328.949 kb on - strand, within GFF2902 at 329.008 kb on + strand, within GFF2902 at 329.008 kb on + strand, within GFF2902 at 329.009 kb on - strand, within GFF2902 at 329.009 kb on - strand, within GFF2902 at 329.020 kb on + strand, within GFF2902 at 329.021 kb on - strand, within GFF2902 at 329.038 kb on + strand, within GFF2902 at 329.038 kb on + strand, within GFF2902 at 329.038 kb on + strand, within GFF2902 at 329.038 kb on + strand, within GFF2902 at 329.039 kb on - strand, within GFF2902 at 329.039 kb on - strand, within GFF2902
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 326,490 + GFF2900 0.62 +0.2 326,491 - GFF2900 0.62 -0.0 326,491 - GFF2900 0.62 -0.7 326,491 - GFF2900 0.62 -0.2 326,491 - GFF2900 0.62 -1.5 326,728 + GFF2900 0.77 +0.8 326,728 + GFF2900 0.77 -0.1 326,728 + GFF2900 0.77 +0.2 326,728 + GFF2900 0.77 -1.2 326,729 - GFF2900 0.77 -0.5 326,729 - GFF2900 0.77 -1.2 326,729 - GFF2900 0.77 +1.7 326,996 - -0.7 326,996 - -0.6 327,029 - -1.5 327,454 - GFF2901 0.32 -0.6 327,649 - GFF2901 0.49 -2.2 327,649 - GFF2901 0.49 -0.5 328,119 + -0.7 328,119 + -0.1 328,283 - GFF2903 0.53 +0.8 328,297 + GFF2903 0.65 +0.4 328,318 + GFF2903 0.82 +0.5 328,320 + GFF2903 0.84 -0.8 328,320 + GFF2903 0.84 -0.7 328,320 + GFF2903 0.84 -0.3 328,320 + GFF2903 0.84 -0.3 328,320 + GFF2903 0.84 -0.4 328,321 - GFF2903 0.85 +0.7 328,321 - GFF2903 0.85 -1.4 328,459 + -1.5 328,459 + +0.2 328,459 + -1.1 328,459 + -0.4 328,459 + -0.6 328,460 - -0.5 328,552 + GFF2902 0.14 -0.3 328,552 + GFF2902 0.14 +0.8 328,552 + GFF2902 0.14 -1.3 328,553 - GFF2902 0.14 -0.1 328,553 - GFF2902 0.14 -1.1 328,585 + GFF2902 0.17 -0.7 328,594 + GFF2902 0.17 -0.3 328,717 + GFF2902 0.25 -0.5 328,717 + GFF2902 0.25 -1.3 328,907 - GFF2902 0.38 -0.6 328,907 - GFF2902 0.38 +0.1 328,941 - GFF2902 0.40 +0.1 328,948 + GFF2902 0.40 +0.0 328,948 + GFF2902 0.40 -1.2 328,948 + GFF2902 0.40 -0.3 328,948 + GFF2902 0.40 -0.7 328,948 + GFF2902 0.40 -1.3 328,949 - GFF2902 0.40 -0.2 328,949 - GFF2902 0.40 +0.7 328,949 - GFF2902 0.40 +1.2 329,008 + GFF2902 0.44 -0.5 329,008 + GFF2902 0.44 -0.5 329,009 - GFF2902 0.44 -0.6 329,009 - GFF2902 0.44 -0.6 329,020 + GFF2902 0.45 -0.6 329,021 - GFF2902 0.45 -0.6 329,038 + GFF2902 0.46 -0.5 329,038 + GFF2902 0.46 +1.5 329,038 + GFF2902 0.46 -0.6 329,038 + GFF2902 0.46 -0.8 329,039 - GFF2902 0.46 -0.3 329,039 - GFF2902 0.46 -0.9
Or see this region's nucleotide sequence