Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2577 and GFF2578 overlap by 4 nucleotides GFF2578 and GFF2579 are separated by 19 nucleotides GFF2579 and GFF2580 are separated by 69 nucleotides
GFF2577 - 7-carboxy-7-deazaguanine synthase (EC 4.3.99.3), at 634,055 to 634,693
GFF2577
GFF2578 - Pantoate--beta-alanine ligase (EC 6.3.2.1), at 634,690 to 635,532
GFF2578
GFF2579 - 3-methyl-2-oxobutanoate hydroxymethyltransferase (EC 2.1.2.11), at 635,552 to 636,445
GFF2579
GFF2580 - L-aspartate oxidase (EC 1.4.3.16), at 636,515 to 638,101
GFF2580
Position (kb) 635
636
637 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.665 kb on + strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.666 kb on - strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.686 kb on + strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.687 kb on - strand at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.823 kb on + strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.824 kb on - strand, within GFF2578 at 634.873 kb on + strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.874 kb on - strand, within GFF2578 at 634.973 kb on + strand, within GFF2578 at 634.974 kb on - strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.090 kb on + strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.091 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.334 kb on - strand, within GFF2578 at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.528 kb on + strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.529 kb on - strand at 635.554 kb on - strand at 635.554 kb on - strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.607 kb on + strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.608 kb on - strand at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.918 kb on + strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 635.919 kb on - strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.084 kb on + strand, within GFF2579 at 636.085 kb on - strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.165 kb on + strand, within GFF2579 at 636.166 kb on - strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.291 kb on + strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.292 kb on - strand, within GFF2579 at 636.399 kb on + strand at 636.696 kb on + strand, within GFF2580 at 636.696 kb on + strand, within GFF2580 at 636.696 kb on + strand, within GFF2580 at 636.697 kb on - strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.780 kb on + strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 636.781 kb on - strand, within GFF2580 at 637.011 kb on + strand, within GFF2580 at 637.011 kb on + strand, within GFF2580 at 637.011 kb on + strand, within GFF2580 at 637.011 kb on + strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.012 kb on - strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.068 kb on + strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.069 kb on - strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.194 kb on + strand, within GFF2580 at 637.195 kb on - strand, within GFF2580 at 637.195 kb on - strand, within GFF2580 at 637.195 kb on - strand, within GFF2580 at 637.195 kb on - strand, within GFF2580 at 637.195 kb on - strand, within GFF2580 at 637.378 kb on - strand, within GFF2580 at 637.378 kb on - strand, within GFF2580 at 637.378 kb on - strand, within GFF2580 at 637.378 kb on - strand, within GFF2580 at 637.378 kb on - strand, within GFF2580
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 634,665 + +1.6 634,665 + +1.0 634,665 + +2.1 634,665 + +0.8 634,666 - +0.8 634,666 - +0.7 634,666 - +1.5 634,666 - +0.5 634,666 - +1.0 634,666 - +1.1 634,666 - +1.0 634,666 - +1.1 634,666 - +0.7 634,666 - +1.7 634,686 + +1.4 634,686 + +0.6 634,686 + +0.5 634,686 + +0.5 634,687 - +0.7 634,687 - +2.5 634,687 - +1.6 634,687 - +1.3 634,687 - -0.1 634,687 - +0.6 634,687 - -0.6 634,687 - +1.0 634,687 - +1.3 634,823 + GFF2578 0.16 -1.4 634,823 + GFF2578 0.16 -0.8 634,823 + GFF2578 0.16 +0.0 634,823 + GFF2578 0.16 +0.2 634,824 - GFF2578 0.16 -1.2 634,824 - GFF2578 0.16 -0.9 634,824 - GFF2578 0.16 -0.2 634,824 - GFF2578 0.16 +0.1 634,824 - GFF2578 0.16 -0.4 634,824 - GFF2578 0.16 -1.3 634,873 + GFF2578 0.22 -2.2 634,874 - GFF2578 0.22 -0.1 634,874 - GFF2578 0.22 -0.2 634,874 - GFF2578 0.22 -1.9 634,973 + GFF2578 0.34 -1.1 634,974 - GFF2578 0.34 -2.0 635,090 + GFF2578 0.47 -1.6 635,090 + GFF2578 0.47 -2.7 635,090 + GFF2578 0.47 -0.9 635,090 + GFF2578 0.47 -1.9 635,090 + GFF2578 0.47 -0.1 635,091 - GFF2578 0.48 +0.5 635,091 - GFF2578 0.48 +0.9 635,091 - GFF2578 0.48 -0.3 635,091 - GFF2578 0.48 -1.1 635,091 - GFF2578 0.48 +0.3 635,334 - GFF2578 0.76 -1.1 635,334 - GFF2578 0.76 -1.7 635,334 - GFF2578 0.76 -0.9 635,334 - GFF2578 0.76 +0.1 635,528 + -2.3 635,528 + +0.1 635,528 + -0.4 635,528 + -0.3 635,528 + -0.7 635,528 + -1.2 635,528 + -0.9 635,528 + +0.9 635,528 + -2.1 635,528 + -3.2 635,529 - -0.1 635,529 - -0.5 635,529 - -0.4 635,529 - +2.3 635,529 - -0.2 635,529 - -1.3 635,529 - -2.1 635,529 - -0.1 635,554 - +1.2 635,554 - +0.4 635,607 + -0.9 635,607 + -0.3 635,607 + -0.8 635,607 + -0.1 635,607 + -0.3 635,607 + +0.0 635,607 + -1.1 635,607 + -0.8 635,607 + -0.9 635,607 + -2.0 635,607 + -0.9 635,607 + -2.0 635,608 - +0.4 635,608 - +0.4 635,608 - -0.5 635,608 - -0.2 635,608 - -0.3 635,608 - -1.4 635,608 - -0.2 635,608 - +0.5 635,608 - +0.4 635,918 + GFF2579 0.41 -1.1 635,918 + GFF2579 0.41 -0.0 635,918 + GFF2579 0.41 +0.1 635,918 + GFF2579 0.41 +0.7 635,918 + GFF2579 0.41 -0.5 635,918 + GFF2579 0.41 -2.0 635,918 + GFF2579 0.41 +0.1 635,918 + GFF2579 0.41 -0.3 635,918 + GFF2579 0.41 -0.9 635,919 - GFF2579 0.41 -0.4 635,919 - GFF2579 0.41 +0.8 635,919 - GFF2579 0.41 -0.6 635,919 - GFF2579 0.41 -1.3 635,919 - GFF2579 0.41 -0.3 635,919 - GFF2579 0.41 +0.1 635,919 - GFF2579 0.41 +0.1 635,919 - GFF2579 0.41 -0.9 635,919 - GFF2579 0.41 -0.9 635,919 - GFF2579 0.41 +0.1 635,919 - GFF2579 0.41 -0.8 635,919 - GFF2579 0.41 -0.3 635,919 - GFF2579 0.41 -1.6 636,084 + GFF2579 0.60 -1.7 636,084 + GFF2579 0.60 -0.9 636,085 - GFF2579 0.60 -0.1 636,165 + GFF2579 0.69 +0.4 636,165 + GFF2579 0.69 -0.6 636,165 + GFF2579 0.69 -2.2 636,166 - GFF2579 0.69 +0.5 636,291 + GFF2579 0.83 -1.4 636,291 + GFF2579 0.83 -0.4 636,292 - GFF2579 0.83 -0.0 636,292 - GFF2579 0.83 -0.7 636,292 - GFF2579 0.83 -0.5 636,399 + -0.5 636,696 + GFF2580 0.11 -2.4 636,696 + GFF2580 0.11 -0.6 636,696 + GFF2580 0.11 +0.6 636,697 - GFF2580 0.11 -0.6 636,780 + GFF2580 0.17 -1.6 636,780 + GFF2580 0.17 -3.2 636,780 + GFF2580 0.17 -0.5 636,780 + GFF2580 0.17 +0.7 636,780 + GFF2580 0.17 -1.4 636,780 + GFF2580 0.17 -1.4 636,780 + GFF2580 0.17 -0.3 636,780 + GFF2580 0.17 +0.9 636,780 + GFF2580 0.17 +0.4 636,780 + GFF2580 0.17 -0.3 636,780 + GFF2580 0.17 +0.1 636,780 + GFF2580 0.17 -0.1 636,780 + GFF2580 0.17 -0.1 636,780 + GFF2580 0.17 -0.1 636,781 - GFF2580 0.17 -0.2 636,781 - GFF2580 0.17 -0.7 636,781 - GFF2580 0.17 -0.6 636,781 - GFF2580 0.17 +0.1 636,781 - GFF2580 0.17 -0.4 636,781 - GFF2580 0.17 -0.2 636,781 - GFF2580 0.17 +0.4 636,781 - GFF2580 0.17 -2.0 636,781 - GFF2580 0.17 +0.2 636,781 - GFF2580 0.17 +1.1 636,781 - GFF2580 0.17 -1.4 636,781 - GFF2580 0.17 -0.3 637,011 + GFF2580 0.31 -0.0 637,011 + GFF2580 0.31 -0.7 637,011 + GFF2580 0.31 +0.3 637,011 + GFF2580 0.31 -0.3 637,012 - GFF2580 0.31 -0.1 637,012 - GFF2580 0.31 +0.1 637,012 - GFF2580 0.31 -1.0 637,012 - GFF2580 0.31 +0.0 637,012 - GFF2580 0.31 -0.4 637,012 - GFF2580 0.31 -0.3 637,068 + GFF2580 0.35 +0.5 637,068 + GFF2580 0.35 +0.0 637,068 + GFF2580 0.35 -1.9 637,068 + GFF2580 0.35 -0.3 637,068 + GFF2580 0.35 -0.3 637,068 + GFF2580 0.35 +0.4 637,068 + GFF2580 0.35 +0.7 637,068 + GFF2580 0.35 -0.4 637,068 + GFF2580 0.35 -0.5 637,069 - GFF2580 0.35 -0.8 637,069 - GFF2580 0.35 -1.7 637,069 - GFF2580 0.35 +1.1 637,069 - GFF2580 0.35 -1.6 637,069 - GFF2580 0.35 -1.1 637,069 - GFF2580 0.35 -0.9 637,069 - GFF2580 0.35 -1.1 637,069 - GFF2580 0.35 +0.0 637,069 - GFF2580 0.35 +0.2 637,069 - GFF2580 0.35 -1.2 637,069 - GFF2580 0.35 -0.1 637,194 + GFF2580 0.43 -0.5 637,194 + GFF2580 0.43 +0.9 637,194 + GFF2580 0.43 +0.0 637,194 + GFF2580 0.43 -0.9 637,194 + GFF2580 0.43 -1.3 637,194 + GFF2580 0.43 -0.2 637,194 + GFF2580 0.43 -2.0 637,194 + GFF2580 0.43 -0.6 637,195 - GFF2580 0.43 +0.1 637,195 - GFF2580 0.43 -0.1 637,195 - GFF2580 0.43 -0.3 637,195 - GFF2580 0.43 +0.9 637,195 - GFF2580 0.43 -1.4 637,378 - GFF2580 0.54 -0.9 637,378 - GFF2580 0.54 -2.4 637,378 - GFF2580 0.54 -0.3 637,378 - GFF2580 0.54 -1.4 637,378 - GFF2580 0.54 -0.5
Or see this region's nucleotide sequence