Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF228 and GFF229 are separated by 27 nucleotides GFF229 and GFF230 are separated by 37 nucleotides GFF230 and GFF231 are separated by 62 nucleotides
GFF228 - ABC transporter, permease protein 1 (cluster 5, nickel/peptides/opines), at 255,680 to 256,720
GFF228
GFF229 - ABC transporter, substrate-binding protein (cluster 5, nickel/peptides/opines), at 256,748 to 258,562
GFF229
GFF230 - Enoyl-[acyl-carrier-protein] reductase [NADH] (EC 1.3.1.9), at 258,600 to 259,382
GFF230
GFF231 - DNA ligase (ATP) (EC 6.5.1.1), at 259,445 to 260,287
GFF231
Position (kb) 256
257
258
259 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 255.765 kb on + strand at 255.765 kb on + strand at 255.765 kb on + strand at 255.765 kb on + strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.766 kb on - strand at 255.790 kb on - strand, within GFF228 at 255.814 kb on - strand, within GFF228 at 255.857 kb on - strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.993 kb on + strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 255.994 kb on - strand, within GFF228 at 256.098 kb on + strand, within GFF228 at 256.099 kb on - strand, within GFF228 at 256.099 kb on - strand, within GFF228 at 256.123 kb on + strand, within GFF228 at 256.230 kb on + strand, within GFF228 at 256.231 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.321 kb on - strand, within GFF228 at 256.333 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228 at 256.486 kb on - strand, within GFF228 at 256.540 kb on - strand, within GFF228 at 256.540 kb on - strand, within GFF228 at 256.608 kb on + strand, within GFF228 at 256.609 kb on - strand, within GFF228 at 256.609 kb on - strand, within GFF228 at 256.609 kb on - strand, within GFF228 at 256.609 kb on - strand, within GFF228 at 256.609 kb on - strand, within GFF228 at 256.710 kb on + strand at 256.710 kb on + strand at 256.710 kb on + strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.711 kb on - strand at 256.749 kb on + strand at 256.749 kb on + strand at 256.749 kb on + strand at 256.749 kb on + strand at 256.749 kb on + strand at 256.749 kb on + strand at 256.750 kb on - strand at 256.750 kb on - strand at 256.750 kb on - strand at 256.851 kb on + strand at 256.851 kb on + strand at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.013 kb on + strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.014 kb on - strand, within GFF229 at 257.142 kb on + strand, within GFF229 at 257.142 kb on + strand, within GFF229 at 257.143 kb on - strand, within GFF229 at 257.143 kb on - strand, within GFF229 at 257.143 kb on - strand, within GFF229 at 257.247 kb on + strand, within GFF229 at 257.248 kb on - strand, within GFF229 at 257.298 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.386 kb on + strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.387 kb on - strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.388 kb on + strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.389 kb on - strand, within GFF229 at 257.523 kb on + strand, within GFF229 at 257.523 kb on + strand, within GFF229 at 257.523 kb on + strand, within GFF229 at 257.523 kb on + strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.524 kb on - strand, within GFF229 at 257.550 kb on + strand, within GFF229 at 257.727 kb on + strand, within GFF229 at 257.727 kb on + strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.728 kb on - strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.790 kb on + strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.791 kb on - strand, within GFF229 at 257.856 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.874 kb on + strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.875 kb on - strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.913 kb on + strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 257.914 kb on - strand, within GFF229 at 258.114 kb on + strand, within GFF229 at 258.114 kb on + strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.115 kb on - strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.129 kb on + strand, within GFF229 at 258.130 kb on - strand, within GFF229 at 258.150 kb on + strand, within GFF229 at 258.150 kb on + strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.151 kb on - strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.372 kb on + strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.373 kb on - strand, within GFF229 at 258.447 kb on + strand at 258.447 kb on + strand at 258.447 kb on + strand at 258.474 kb on + strand at 258.474 kb on + strand at 258.475 kb on - strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.498 kb on + strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.499 kb on - strand at 258.501 kb on - strand at 258.641 kb on - strand at 258.641 kb on - strand at 258.664 kb on - strand at 259.036 kb on - strand, within GFF230 at 259.335 kb on + strand at 259.381 kb on - strand at 259.427 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.431 kb on + strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.432 kb on - strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.438 kb on + strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.439 kb on - strand at 259.547 kb on + strand, within GFF231 at 259.548 kb on - strand, within GFF231 at 259.548 kb on - strand, within GFF231
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 255,765 + -1.7 255,765 + -1.0 255,765 + -1.2 255,765 + -1.4 255,766 - -1.1 255,766 - -1.1 255,766 - -0.5 255,766 - -0.8 255,766 - -0.9 255,790 - GFF228 0.11 -0.9 255,814 - GFF228 0.13 -1.9 255,857 - GFF228 0.17 -1.0 255,993 + GFF228 0.30 -0.1 255,993 + GFF228 0.30 -2.3 255,993 + GFF228 0.30 +0.2 255,993 + GFF228 0.30 -0.2 255,993 + GFF228 0.30 -1.4 255,993 + GFF228 0.30 -0.1 255,994 - GFF228 0.30 -2.0 255,994 - GFF228 0.30 -0.9 255,994 - GFF228 0.30 -0.5 255,994 - GFF228 0.30 -0.3 255,994 - GFF228 0.30 -1.2 255,994 - GFF228 0.30 -0.6 255,994 - GFF228 0.30 -1.0 256,098 + GFF228 0.40 +1.3 256,099 - GFF228 0.40 -1.5 256,099 - GFF228 0.40 -0.9 256,123 + GFF228 0.43 -0.5 256,230 + GFF228 0.53 -0.9 256,231 - GFF228 0.53 +0.5 256,321 - GFF228 0.62 +0.3 256,321 - GFF228 0.62 -0.7 256,321 - GFF228 0.62 -1.3 256,321 - GFF228 0.62 -1.2 256,333 - GFF228 0.63 -0.9 256,486 - GFF228 0.77 +1.3 256,486 - GFF228 0.77 -0.8 256,486 - GFF228 0.77 -0.9 256,540 - GFF228 0.83 -1.1 256,540 - GFF228 0.83 -1.3 256,608 + GFF228 0.89 -0.0 256,609 - GFF228 0.89 +0.1 256,609 - GFF228 0.89 -0.1 256,609 - GFF228 0.89 -0.1 256,609 - GFF228 0.89 -1.6 256,609 - GFF228 0.89 -1.4 256,710 + -0.1 256,710 + +0.3 256,710 + +1.4 256,711 - +0.4 256,711 - -1.3 256,711 - +0.1 256,711 - -0.2 256,711 - -0.6 256,711 - -0.3 256,711 - -0.6 256,711 - +0.5 256,711 - -1.0 256,711 - -1.3 256,749 + -1.0 256,749 + +0.1 256,749 + -0.8 256,749 + -1.7 256,749 + -0.3 256,749 + +0.1 256,750 - -0.9 256,750 - +1.3 256,750 - +0.4 256,851 + -0.8 256,851 + -1.2 257,013 + GFF229 0.15 +0.5 257,013 + GFF229 0.15 -0.7 257,013 + GFF229 0.15 -1.9 257,013 + GFF229 0.15 -1.1 257,013 + GFF229 0.15 +0.3 257,013 + GFF229 0.15 -0.3 257,013 + GFF229 0.15 -1.5 257,013 + GFF229 0.15 -0.4 257,013 + GFF229 0.15 -0.6 257,013 + GFF229 0.15 +0.8 257,014 - GFF229 0.15 -1.6 257,014 - GFF229 0.15 -1.0 257,014 - GFF229 0.15 -1.0 257,014 - GFF229 0.15 -1.2 257,014 - GFF229 0.15 -0.6 257,014 - GFF229 0.15 +0.6 257,014 - GFF229 0.15 -1.3 257,014 - GFF229 0.15 +0.1 257,014 - GFF229 0.15 +0.7 257,142 + GFF229 0.22 +1.0 257,142 + GFF229 0.22 -0.7 257,143 - GFF229 0.22 -2.6 257,143 - GFF229 0.22 -0.2 257,143 - GFF229 0.22 -0.2 257,247 + GFF229 0.27 -0.8 257,248 - GFF229 0.28 -0.8 257,298 + GFF229 0.30 -1.6 257,386 + GFF229 0.35 +0.9 257,386 + GFF229 0.35 -0.8 257,386 + GFF229 0.35 -0.2 257,386 + GFF229 0.35 -1.2 257,386 + GFF229 0.35 -0.8 257,386 + GFF229 0.35 -0.3 257,387 - GFF229 0.35 -1.0 257,387 - GFF229 0.35 -1.3 257,387 - GFF229 0.35 +0.1 257,387 - GFF229 0.35 +0.4 257,387 - GFF229 0.35 -0.3 257,387 - GFF229 0.35 -1.1 257,388 + GFF229 0.35 -0.3 257,388 + GFF229 0.35 -0.4 257,388 + GFF229 0.35 -0.5 257,388 + GFF229 0.35 -0.2 257,388 + GFF229 0.35 -0.9 257,388 + GFF229 0.35 -0.7 257,388 + GFF229 0.35 -2.7 257,388 + GFF229 0.35 -0.4 257,388 + GFF229 0.35 -2.8 257,388 + GFF229 0.35 +0.1 257,388 + GFF229 0.35 -0.8 257,388 + GFF229 0.35 -0.4 257,388 + GFF229 0.35 -0.6 257,388 + GFF229 0.35 -0.5 257,389 - GFF229 0.35 -0.1 257,389 - GFF229 0.35 -0.9 257,389 - GFF229 0.35 -0.3 257,389 - GFF229 0.35 -2.1 257,389 - GFF229 0.35 -1.0 257,389 - GFF229 0.35 -0.6 257,389 - GFF229 0.35 -0.3 257,389 - GFF229 0.35 -0.2 257,389 - GFF229 0.35 +0.5 257,389 - GFF229 0.35 +0.5 257,389 - GFF229 0.35 -1.1 257,389 - GFF229 0.35 -1.5 257,389 - GFF229 0.35 -0.6 257,389 - GFF229 0.35 +0.1 257,389 - GFF229 0.35 -0.7 257,389 - GFF229 0.35 -2.3 257,523 + GFF229 0.43 +0.5 257,523 + GFF229 0.43 -1.7 257,523 + GFF229 0.43 -1.2 257,523 + GFF229 0.43 -1.9 257,524 - GFF229 0.43 -2.0 257,524 - GFF229 0.43 -1.7 257,524 - GFF229 0.43 -1.2 257,524 - GFF229 0.43 -0.9 257,524 - GFF229 0.43 -1.9 257,524 - GFF229 0.43 -0.9 257,550 + GFF229 0.44 +0.3 257,727 + GFF229 0.54 -1.5 257,727 + GFF229 0.54 -0.8 257,728 - GFF229 0.54 +0.1 257,728 - GFF229 0.54 +1.9 257,728 - GFF229 0.54 -2.2 257,790 + GFF229 0.57 +0.4 257,790 + GFF229 0.57 -0.3 257,790 + GFF229 0.57 -1.7 257,791 - GFF229 0.57 -0.6 257,791 - GFF229 0.57 -1.0 257,791 - GFF229 0.57 -2.0 257,791 - GFF229 0.57 -0.9 257,856 + GFF229 0.61 -0.9 257,874 + GFF229 0.62 -1.1 257,874 + GFF229 0.62 -1.5 257,874 + GFF229 0.62 -0.9 257,874 + GFF229 0.62 -0.9 257,874 + GFF229 0.62 -0.6 257,875 - GFF229 0.62 -1.7 257,875 - GFF229 0.62 -0.7 257,875 - GFF229 0.62 -1.4 257,875 - GFF229 0.62 -0.1 257,875 - GFF229 0.62 +0.1 257,875 - GFF229 0.62 -0.6 257,875 - GFF229 0.62 +0.4 257,875 - GFF229 0.62 -1.3 257,875 - GFF229 0.62 +0.4 257,913 + GFF229 0.64 -1.7 257,913 + GFF229 0.64 -0.6 257,913 + GFF229 0.64 -0.3 257,913 + GFF229 0.64 -1.5 257,913 + GFF229 0.64 -0.5 257,913 + GFF229 0.64 -0.9 257,913 + GFF229 0.64 -0.8 257,914 - GFF229 0.64 +0.2 257,914 - GFF229 0.64 -3.0 257,914 - GFF229 0.64 -0.5 257,914 - GFF229 0.64 +0.0 258,114 + GFF229 0.75 -1.0 258,114 + GFF229 0.75 +1.0 258,115 - GFF229 0.75 -1.1 258,115 - GFF229 0.75 -0.7 258,115 - GFF229 0.75 -2.0 258,129 + GFF229 0.76 -1.8 258,129 + GFF229 0.76 -1.1 258,129 + GFF229 0.76 -0.3 258,129 + GFF229 0.76 +0.3 258,129 + GFF229 0.76 +0.3 258,130 - GFF229 0.76 -1.3 258,150 + GFF229 0.77 -0.7 258,150 + GFF229 0.77 -0.8 258,151 - GFF229 0.77 +2.3 258,151 - GFF229 0.77 -1.4 258,151 - GFF229 0.77 -0.6 258,151 - GFF229 0.77 -0.3 258,151 - GFF229 0.77 +0.1 258,151 - GFF229 0.77 -1.5 258,372 + GFF229 0.89 -0.2 258,372 + GFF229 0.89 -2.7 258,372 + GFF229 0.89 -0.6 258,372 + GFF229 0.89 +0.4 258,372 + GFF229 0.89 -1.2 258,372 + GFF229 0.89 +0.3 258,372 + GFF229 0.89 -1.4 258,372 + GFF229 0.89 +0.2 258,373 - GFF229 0.90 -0.6 258,373 - GFF229 0.90 -0.6 258,373 - GFF229 0.90 -1.3 258,373 - GFF229 0.90 -0.3 258,373 - GFF229 0.90 +0.7 258,373 - GFF229 0.90 -1.3 258,447 + -1.1 258,447 + -1.5 258,447 + -1.3 258,474 + -3.3 258,474 + -0.3 258,475 - -0.0 258,498 + -2.3 258,498 + -0.0 258,498 + +0.4 258,498 + +0.5 258,498 + -0.6 258,499 - -1.1 258,499 - +1.0 258,499 - -0.8 258,499 - -2.6 258,501 - -0.1 258,641 - -1.3 258,641 - -0.7 258,664 - -1.5 259,036 - GFF230 0.56 -1.7 259,335 + -0.7 259,381 - +0.5 259,427 + -1.0 259,431 + +0.4 259,431 + -0.2 259,431 + -1.3 259,431 + -0.5 259,431 + +0.9 259,431 + +0.1 259,431 + -1.9 259,432 - +0.4 259,432 - -0.1 259,432 - -1.3 259,432 - -0.4 259,438 + +0.7 259,438 + -0.1 259,438 + -0.6 259,438 + -1.8 259,439 - +0.3 259,439 - -1.7 259,439 - -0.6 259,439 - -0.6 259,547 + GFF231 0.12 -0.4 259,548 - GFF231 0.12 +0.9 259,548 - GFF231 0.12 +0.5
Or see this region's nucleotide sequence