Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2244 and GFF2245 overlap by 4 nucleotides GFF2245 and GFF2246 are separated by 36 nucleotides GFF2246 and GFF2247 are separated by 10 nucleotides GFF2247 and GFF2248 are separated by 35 nucleotides
GFF2244 - hypothetical protein, at 301,784 to 302,647
GFF2244
GFF2245 - Glycerol-3-phosphate ABC transporter, permease protein UgpE (TC 3.A.1.1.3), at 302,644 to 303,219
GFF2245
GFF2246 - cellular communication/signal transduction, at 303,256 to 303,612
GFF2246
GFF2247 - hypothetical protein, at 303,623 to 304,150
GFF2247
GFF2248 - Transcriptional regulatory protein RtcR, at 304,186 to 305,808
GFF2248
Position (kb) 303
304
305 Strain fitness (log2 ratio) -2
-1
0
1 at 302.885 kb on + strand, within GFF2245 at 302.886 kb on - strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.891 kb on + strand, within GFF2245 at 302.969 kb on + strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 302.970 kb on - strand, within GFF2245 at 303.132 kb on - strand, within GFF2245 at 303.203 kb on - strand at 303.203 kb on - strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.206 kb on + strand at 303.320 kb on + strand, within GFF2246 at 303.320 kb on + strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.321 kb on - strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.326 kb on + strand, within GFF2246 at 303.327 kb on - strand, within GFF2246 at 303.716 kb on + strand, within GFF2247 at 303.717 kb on - strand, within GFF2247 at 304.162 kb on + strand at 304.241 kb on + strand at 304.241 kb on + strand at 304.625 kb on + strand, within GFF2248 at 304.748 kb on + strand, within GFF2248 at 304.748 kb on + strand, within GFF2248 at 304.749 kb on - strand, within GFF2248 at 304.749 kb on - strand, within GFF2248 at 304.884 kb on - strand, within GFF2248
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 302,885 + GFF2245 0.42 -0.2 302,886 - GFF2245 0.42 -0.8 302,891 + GFF2245 0.43 +1.6 302,891 + GFF2245 0.43 -1.3 302,969 + GFF2245 0.56 -1.0 302,970 - GFF2245 0.57 -0.2 302,970 - GFF2245 0.57 +0.3 303,132 - GFF2245 0.85 -0.9 303,203 - -0.2 303,203 - -0.2 303,206 + -0.2 303,206 + +0.7 303,206 + -0.2 303,320 + GFF2246 0.18 +0.5 303,320 + GFF2246 0.18 -0.2 303,321 - GFF2246 0.18 -0.4 303,321 - GFF2246 0.18 -0.5 303,326 + GFF2246 0.20 -0.3 303,326 + GFF2246 0.20 +0.3 303,327 - GFF2246 0.20 +0.2 303,716 + GFF2247 0.18 +1.4 303,717 - GFF2247 0.18 -0.3 304,162 + +1.4 304,241 + -0.0 304,241 + -0.2 304,625 + GFF2248 0.27 -0.8 304,748 + GFF2248 0.35 -0.7 304,748 + GFF2248 0.35 -0.4 304,749 - GFF2248 0.35 -1.8 304,749 - GFF2248 0.35 -0.7 304,884 - GFF2248 0.43 -0.2
Or see this region's nucleotide sequence