Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2229 and GFF2230 are separated by 136 nucleotides GFF2230 and GFF2231 overlap by 4 nucleotides GFF2231 and GFF2232 are separated by 34 nucleotides
GFF2229 - Pca regulon regulatory protein PcaR, at 291,162 to 291,947
GFF2229
GFF2230 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6), at 292,084 to 292,785
GFF2230
GFF2231 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6), at 292,782 to 293,429
GFF2231
GFF2232 - Acetyl-CoA acetyltransferase (EC 2.3.1.9) @ 3-oxoadipyl-CoA thiolase (EC 2.3.1.174), at 293,464 to 294,669
GFF2232
Position (kb)
292
293 Strain fitness (log2 ratio)
-1
0
1
2 at 291.508 kb on + strand, within GFF2229 at 291.577 kb on + strand, within GFF2229 at 291.578 kb on - strand, within GFF2229 at 291.688 kb on + strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.689 kb on - strand, within GFF2229 at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.898 kb on + strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 291.899 kb on - strand at 292.460 kb on - strand, within GFF2230 at 292.592 kb on - strand, within GFF2230 at 292.653 kb on + strand, within GFF2230 at 292.794 kb on + strand at 292.794 kb on + strand at 292.794 kb on + strand at 292.795 kb on - strand at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 292.987 kb on - strand, within GFF2231 at 293.445 kb on + strand at 293.445 kb on + strand at 293.445 kb on + strand at 293.446 kb on - strand at 293.515 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 291,508 + GFF2229 0.44 +0.2 291,577 + GFF2229 0.53 -0.2 291,578 - GFF2229 0.53 -0.3 291,688 + GFF2229 0.67 -0.2 291,689 - GFF2229 0.67 -1.7 291,689 - GFF2229 0.67 +0.6 291,689 - GFF2229 0.67 -0.6 291,898 + +1.8 291,898 + -1.4 291,898 + -0.3 291,898 + -0.4 291,898 + -0.5 291,898 + -0.2 291,898 + -0.8 291,898 + +0.7 291,898 + +0.1 291,898 + -0.8 291,898 + -0.3 291,899 - +0.5 291,899 - -0.5 291,899 - -0.3 291,899 - -0.0 291,899 - -0.3 291,899 - -0.3 291,899 - +0.8 292,460 - GFF2230 0.54 +0.4 292,592 - GFF2230 0.72 -0.7 292,653 + GFF2230 0.81 +0.2 292,794 + +0.5 292,794 + +1.4 292,794 + +0.2 292,795 - -0.2 292,987 - GFF2231 0.32 +0.2 292,987 - GFF2231 0.32 +0.5 292,987 - GFF2231 0.32 -0.1 292,987 - GFF2231 0.32 +0.7 293,445 + -0.0 293,445 + +1.2 293,445 + +1.2 293,446 - -0.1 293,515 + -0.5
Or see this region's nucleotide sequence