Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2149 and GFF2150 are separated by 129 nucleotides GFF2150 and GFF2151 are separated by 84 nucleotides GFF2151 and GFF2152 overlap by 1 nucleotides GFF2152 and GFF2153 are separated by 19 nucleotides GFF2153 and GFF2154 are separated by 42 nucleotides
GFF2149 - Hydroxymethylpyrimidine phosphate kinase ThiD (EC 2.7.4.7), at 197,974 to 198,936
GFF2149
GFF2150 - Rubredoxin, at 199,066 to 199,218
GFF2150
GFF2151 - twitching motility protein PilG, at 199,303 to 199,701
GFF2151
GFF2152 - twitching motility protein PilH, at 199,701 to 200,066
GFF2152
GFF2153 - type IV pili signal transduction protein PilI, at 200,086 to 200,619
GFF2153
GFF2154 - twitching motility protein PilJ, at 200,662 to 202,968
GFF2154
Position (kb) 199
200
201 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 198.720 kb on - strand, within GFF2149 at 198.885 kb on + strand at 198.885 kb on + strand at 198.885 kb on + strand at 198.885 kb on + strand at 198.886 kb on - strand at 198.910 kb on - strand at 198.910 kb on - strand at 198.910 kb on - strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.922 kb on + strand at 198.923 kb on - strand at 198.923 kb on - strand at 198.923 kb on - strand at 198.923 kb on - strand at 198.923 kb on - strand at 198.923 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.927 kb on - strand at 198.936 kb on + strand at 198.936 kb on + strand at 198.936 kb on + strand at 198.937 kb on - strand at 198.937 kb on - strand at 198.937 kb on - strand at 198.937 kb on - strand at 198.967 kb on + strand at 198.967 kb on + strand at 198.968 kb on - strand at 198.968 kb on - strand at 198.968 kb on - strand at 198.968 kb on - strand at 198.980 kb on + strand at 198.981 kb on - strand at 198.981 kb on - strand at 198.981 kb on - strand at 198.981 kb on - strand at 198.981 kb on - strand at 198.991 kb on - strand at 198.998 kb on + strand at 198.998 kb on + strand at 198.998 kb on + strand at 198.998 kb on + strand at 198.998 kb on + strand at 198.999 kb on - strand at 198.999 kb on - strand at 199.004 kb on + strand at 199.004 kb on + strand at 199.005 kb on - strand at 199.005 kb on - strand at 199.005 kb on - strand at 199.005 kb on - strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.008 kb on + strand at 199.009 kb on - strand at 199.009 kb on - strand at 199.009 kb on - strand at 199.009 kb on - strand at 199.023 kb on + strand at 199.024 kb on - strand at 199.039 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.229 kb on + strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.230 kb on - strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.233 kb on + strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.234 kb on - strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.239 kb on + strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.240 kb on - strand at 199.324 kb on + strand at 199.324 kb on + strand at 199.324 kb on + strand at 199.325 kb on - strand at 199.325 kb on - strand at 199.501 kb on + strand, within GFF2151 at 199.501 kb on + strand, within GFF2151 at 199.502 kb on - strand, within GFF2151 at 199.502 kb on - strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.580 kb on + strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.581 kb on - strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.621 kb on + strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.622 kb on - strand, within GFF2151 at 199.699 kb on + strand at 199.699 kb on + strand at 199.699 kb on + strand at 199.699 kb on + strand at 199.700 kb on - strand at 199.700 kb on - strand at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.923 kb on + strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.924 kb on - strand, within GFF2152 at 199.931 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.007 kb on + strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.008 kb on - strand, within GFF2152 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.203 kb on + strand, within GFF2153 at 200.204 kb on - strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.266 kb on + strand, within GFF2153 at 200.267 kb on - strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.314 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.533 kb on + strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.534 kb on - strand, within GFF2153 at 200.607 kb on + strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.608 kb on - strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.617 kb on + strand at 200.618 kb on - strand at 200.618 kb on - strand at 200.693 kb on + strand at 200.693 kb on + strand at 200.694 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 198,720 - GFF2149 0.77 -0.2 198,885 + +0.2 198,885 + -0.8 198,885 + -0.2 198,885 + -0.4 198,886 - +0.4 198,910 - -0.7 198,910 - -2.4 198,910 - -0.4 198,922 + -1.7 198,922 + -0.2 198,922 + -0.7 198,922 + +1.2 198,922 + -1.2 198,922 + -0.4 198,922 + -0.4 198,922 + -0.5 198,922 + -1.2 198,922 + -1.6 198,922 + +1.0 198,923 - +0.6 198,923 - -0.2 198,923 - -0.9 198,923 - -0.4 198,923 - -0.1 198,923 - -0.6 198,927 - -0.6 198,927 - -0.2 198,927 - -0.2 198,927 - -0.1 198,927 - -1.4 198,927 - -0.6 198,927 - -1.6 198,927 - -0.1 198,927 - -0.3 198,927 - -0.2 198,927 - -0.1 198,927 - +1.1 198,936 + -0.6 198,936 + +0.8 198,936 + -0.2 198,937 - -2.3 198,937 - -0.1 198,937 - +0.1 198,937 - +0.1 198,967 + -0.4 198,967 + +0.3 198,968 - -0.3 198,968 - -1.4 198,968 - -1.5 198,968 - -0.3 198,980 + -2.3 198,981 - +0.0 198,981 - -0.3 198,981 - -0.4 198,981 - -0.0 198,981 - +1.4 198,991 - +0.5 198,998 + -0.2 198,998 + -0.7 198,998 + +0.2 198,998 + -2.9 198,998 + -1.3 198,999 - -0.6 198,999 - -0.9 199,004 + -0.4 199,004 + -0.6 199,005 - -0.5 199,005 - -0.7 199,005 - +1.4 199,005 - -0.1 199,008 + -0.5 199,008 + -1.2 199,008 + -0.2 199,008 + -2.0 199,008 + -0.4 199,008 + +0.3 199,009 - -0.7 199,009 - +3.8 199,009 - -0.5 199,009 - -0.2 199,023 + -0.9 199,024 - -0.8 199,039 + +0.4 199,229 + -0.0 199,229 + -0.5 199,229 + +1.2 199,229 + +1.3 199,229 + -1.6 199,229 + +0.4 199,229 + -0.6 199,229 + -0.2 199,229 + -0.5 199,229 + +1.6 199,229 + +0.4 199,229 + -1.0 199,229 + -0.7 199,229 + +0.1 199,229 + -0.5 199,229 + -0.3 199,229 + -0.7 199,229 + -1.4 199,229 + -0.0 199,229 + +0.6 199,230 - -0.3 199,230 - +0.6 199,230 - -0.2 199,230 - +0.1 199,230 - -1.2 199,230 - +0.3 199,230 - +0.1 199,230 - +0.0 199,230 - +0.5 199,230 - -0.5 199,230 - -0.5 199,230 - -0.6 199,230 - +0.5 199,230 - -0.3 199,233 + -1.1 199,233 + -0.4 199,233 + +0.2 199,233 + -1.2 199,233 + -0.8 199,233 + +0.0 199,233 + -0.0 199,233 + +0.6 199,233 + +0.2 199,233 + -0.5 199,233 + -0.8 199,233 + -1.4 199,233 + +0.1 199,233 + -0.4 199,233 + -0.8 199,233 + -0.3 199,233 + +0.2 199,233 + -0.4 199,233 + -0.0 199,233 + -0.1 199,234 - -0.2 199,234 - -0.1 199,234 - +1.5 199,234 - +0.4 199,234 - -0.6 199,234 - +0.8 199,234 - +0.1 199,234 - +1.8 199,234 - -0.3 199,234 - +0.1 199,234 - +0.9 199,234 - +1.0 199,234 - -0.5 199,234 - +0.1 199,234 - -0.5 199,234 - -0.5 199,234 - -0.2 199,234 - -0.2 199,234 - -2.8 199,234 - +0.3 199,234 - -0.7 199,234 - +0.2 199,234 - +0.8 199,234 - +0.3 199,234 - -0.6 199,239 + -0.3 199,239 + -0.6 199,239 + -0.2 199,239 + -0.0 199,239 + +0.2 199,240 - -0.9 199,240 - -0.4 199,240 - -0.0 199,240 - -0.8 199,240 - +0.4 199,240 - +0.3 199,324 + -1.1 199,324 + -0.9 199,324 + +0.1 199,325 - -0.3 199,325 - -0.7 199,501 + GFF2151 0.50 -2.2 199,501 + GFF2151 0.50 -3.5 199,502 - GFF2151 0.50 -1.8 199,502 - GFF2151 0.50 +0.8 199,580 + GFF2151 0.69 -0.3 199,580 + GFF2151 0.69 -0.0 199,580 + GFF2151 0.69 -1.6 199,580 + GFF2151 0.69 -2.1 199,580 + GFF2151 0.69 -1.4 199,580 + GFF2151 0.69 -2.0 199,581 - GFF2151 0.70 -0.9 199,581 - GFF2151 0.70 -0.2 199,581 - GFF2151 0.70 -0.9 199,581 - GFF2151 0.70 -0.6 199,581 - GFF2151 0.70 -1.9 199,581 - GFF2151 0.70 -2.1 199,581 - GFF2151 0.70 -0.8 199,581 - GFF2151 0.70 -0.9 199,581 - GFF2151 0.70 -0.6 199,581 - GFF2151 0.70 -0.8 199,621 + GFF2151 0.80 -1.3 199,621 + GFF2151 0.80 -0.5 199,621 + GFF2151 0.80 -1.5 199,621 + GFF2151 0.80 -3.1 199,621 + GFF2151 0.80 -1.0 199,621 + GFF2151 0.80 -1.7 199,622 - GFF2151 0.80 +0.4 199,622 - GFF2151 0.80 -0.3 199,622 - GFF2151 0.80 -0.2 199,622 - GFF2151 0.80 -0.9 199,622 - GFF2151 0.80 +0.4 199,622 - GFF2151 0.80 -0.2 199,622 - GFF2151 0.80 -0.0 199,699 + -0.3 199,699 + +0.4 199,699 + +0.2 199,699 + -0.2 199,700 - -1.1 199,700 - -0.3 199,923 + GFF2152 0.61 -0.0 199,923 + GFF2152 0.61 -0.1 199,923 + GFF2152 0.61 +1.0 199,923 + GFF2152 0.61 -0.1 199,923 + GFF2152 0.61 -0.6 199,923 + GFF2152 0.61 -0.6 199,923 + GFF2152 0.61 -1.2 199,923 + GFF2152 0.61 +2.0 199,924 - GFF2152 0.61 -0.8 199,924 - GFF2152 0.61 -0.1 199,924 - GFF2152 0.61 -1.1 199,924 - GFF2152 0.61 -0.1 199,924 - GFF2152 0.61 +0.1 199,924 - GFF2152 0.61 +0.2 199,924 - GFF2152 0.61 +0.3 199,931 + GFF2152 0.63 +0.8 200,007 + GFF2152 0.84 +0.1 200,007 + GFF2152 0.84 -0.3 200,007 + GFF2152 0.84 +0.5 200,007 + GFF2152 0.84 -0.3 200,007 + GFF2152 0.84 +0.4 200,008 - GFF2152 0.84 +0.8 200,008 - GFF2152 0.84 -0.1 200,008 - GFF2152 0.84 -0.2 200,008 - GFF2152 0.84 +0.2 200,008 - GFF2152 0.84 -0.7 200,008 - GFF2152 0.84 -0.2 200,008 - GFF2152 0.84 -0.2 200,203 + GFF2153 0.22 -0.1 200,203 + GFF2153 0.22 +0.5 200,203 + GFF2153 0.22 +0.5 200,203 + GFF2153 0.22 +1.8 200,204 - GFF2153 0.22 -0.2 200,266 + GFF2153 0.34 -0.1 200,266 + GFF2153 0.34 +0.2 200,266 + GFF2153 0.34 +0.8 200,266 + GFF2153 0.34 +0.4 200,267 - GFF2153 0.34 -0.2 200,314 + GFF2153 0.43 -0.5 200,314 + GFF2153 0.43 -0.2 200,314 + GFF2153 0.43 -0.3 200,533 + GFF2153 0.84 +0.1 200,533 + GFF2153 0.84 +0.4 200,533 + GFF2153 0.84 +0.1 200,533 + GFF2153 0.84 -0.1 200,533 + GFF2153 0.84 -0.3 200,533 + GFF2153 0.84 +0.0 200,533 + GFF2153 0.84 +0.2 200,534 - GFF2153 0.84 -0.7 200,534 - GFF2153 0.84 +0.9 200,534 - GFF2153 0.84 -0.0 200,534 - GFF2153 0.84 -0.3 200,534 - GFF2153 0.84 +0.0 200,534 - GFF2153 0.84 +0.0 200,534 - GFF2153 0.84 -0.4 200,534 - GFF2153 0.84 -1.2 200,534 - GFF2153 0.84 -0.1 200,607 + -1.5 200,608 - -0.2 200,608 - +0.0 200,608 - +0.4 200,608 - +1.0 200,608 - +0.4 200,617 + +0.1 200,617 + +1.0 200,617 + -0.5 200,617 + +1.3 200,618 - -0.5 200,618 - -0.4 200,693 + -1.2 200,693 + -0.2 200,694 - -0.8
Or see this region's nucleotide sequence