Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2075 and GFF2076 are separated by 24 nucleotides GFF2076 and GFF2077 overlap by 4 nucleotides
GFF2075 - BUG/TctC family periplasmic protein, at 121,166 to 122,161
GFF2075
GFF2076 - Alkyl sulfatase and related hydrolases, MBL-fold metallo-hydrolase superfamily, at 122,186 to 123,457
GFF2076
GFF2077 - Acetyl-CoA synthetase (ADP-forming) alpha and beta chains, putative, at 123,454 to 124,794
GFF2077
Position (kb)
122
123
124 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.395 kb on + strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.396 kb on - strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.587 kb on + strand, within GFF2075 at 121.588 kb on - strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.674 kb on + strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.675 kb on - strand, within GFF2075 at 121.704 kb on + strand, within GFF2075 at 121.827 kb on + strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.828 kb on - strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 121.860 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.061 kb on + strand, within GFF2075 at 122.062 kb on - strand at 122.062 kb on - strand at 122.244 kb on + strand at 122.245 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.263 kb on - strand at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.532 kb on + strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.533 kb on - strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.550 kb on + strand, within GFF2076 at 122.565 kb on + strand, within GFF2076 at 122.566 kb on - strand, within GFF2076 at 122.971 kb on + strand, within GFF2076 at 122.972 kb on + strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 122.973 kb on - strand, within GFF2076 at 123.198 kb on + strand, within GFF2076 at 123.198 kb on + strand, within GFF2076 at 123.198 kb on + strand, within GFF2076 at 123.199 kb on - strand, within GFF2076 at 123.199 kb on - strand, within GFF2076 at 123.199 kb on - strand, within GFF2076 at 123.219 kb on + strand, within GFF2076 at 123.220 kb on - strand, within GFF2076 at 123.220 kb on - strand, within GFF2076 at 123.220 kb on - strand, within GFF2076 at 123.220 kb on - strand, within GFF2076 at 123.220 kb on - strand, within GFF2076 at 123.527 kb on + strand at 123.527 kb on + strand at 123.545 kb on + strand at 123.545 kb on + strand at 123.547 kb on + strand at 123.617 kb on + strand, within GFF2077 at 123.617 kb on + strand, within GFF2077 at 123.617 kb on + strand, within GFF2077 at 123.617 kb on + strand, within GFF2077 at 123.617 kb on + strand, within GFF2077 at 123.617 kb on + strand, within GFF2077 at 123.618 kb on - strand, within GFF2077 at 123.677 kb on + strand, within GFF2077 at 123.677 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.707 kb on + strand, within GFF2077 at 123.708 kb on - strand, within GFF2077 at 123.708 kb on - strand, within GFF2077 at 123.722 kb on + strand, within GFF2077 at 123.722 kb on + strand, within GFF2077 at 123.722 kb on + strand, within GFF2077 at 123.722 kb on + strand, within GFF2077 at 123.722 kb on + strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.723 kb on - strand, within GFF2077 at 123.785 kb on + strand, within GFF2077 at 123.785 kb on + strand, within GFF2077 at 123.785 kb on + strand, within GFF2077 at 123.785 kb on + strand, within GFF2077 at 123.786 kb on - strand, within GFF2077 at 123.872 kb on + strand, within GFF2077 at 123.872 kb on + strand, within GFF2077 at 123.873 kb on - strand, within GFF2077 at 123.873 kb on - strand, within GFF2077
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 121,395 + GFF2075 0.23 +0.2 121,395 + GFF2075 0.23 -1.6 121,395 + GFF2075 0.23 -0.8 121,395 + GFF2075 0.23 -0.1 121,395 + GFF2075 0.23 -0.6 121,396 - GFF2075 0.23 -0.0 121,396 - GFF2075 0.23 +0.2 121,396 - GFF2075 0.23 +0.5 121,396 - GFF2075 0.23 -1.5 121,587 + GFF2075 0.42 -0.5 121,587 + GFF2075 0.42 -0.0 121,588 - GFF2075 0.42 +0.3 121,674 + GFF2075 0.51 +0.1 121,674 + GFF2075 0.51 -0.4 121,674 + GFF2075 0.51 -0.0 121,674 + GFF2075 0.51 -0.2 121,674 + GFF2075 0.51 +0.0 121,675 - GFF2075 0.51 -0.2 121,675 - GFF2075 0.51 -0.0 121,675 - GFF2075 0.51 +0.4 121,675 - GFF2075 0.51 -1.4 121,675 - GFF2075 0.51 +0.1 121,675 - GFF2075 0.51 -0.4 121,704 + GFF2075 0.54 +0.4 121,827 + GFF2075 0.66 +0.0 121,828 - GFF2075 0.66 +0.1 121,828 - GFF2075 0.66 -0.4 121,860 + GFF2075 0.70 +1.8 121,860 + GFF2075 0.70 -2.9 121,860 + GFF2075 0.70 -0.4 121,860 + GFF2075 0.70 -2.2 121,860 + GFF2075 0.70 -0.8 122,061 + GFF2075 0.90 -0.0 122,061 + GFF2075 0.90 -0.2 122,062 - -1.1 122,062 - -0.2 122,244 + -0.0 122,245 - +0.2 122,263 - -0.1 122,263 - -2.0 122,263 - -0.3 122,263 - -0.5 122,263 - -0.3 122,263 - -0.1 122,532 + GFF2076 0.27 -2.0 122,532 + GFF2076 0.27 -1.5 122,532 + GFF2076 0.27 -0.4 122,532 + GFF2076 0.27 -0.5 122,533 - GFF2076 0.27 -1.9 122,533 - GFF2076 0.27 -0.3 122,533 - GFF2076 0.27 -1.5 122,550 + GFF2076 0.29 +0.4 122,550 + GFF2076 0.29 -0.4 122,550 + GFF2076 0.29 +0.2 122,565 + GFF2076 0.30 +0.3 122,566 - GFF2076 0.30 +1.8 122,971 + GFF2076 0.62 -0.4 122,972 + GFF2076 0.62 +0.9 122,973 - GFF2076 0.62 -0.8 122,973 - GFF2076 0.62 +1.2 122,973 - GFF2076 0.62 -0.8 123,198 + GFF2076 0.80 +1.8 123,198 + GFF2076 0.80 -0.8 123,198 + GFF2076 0.80 +0.4 123,199 - GFF2076 0.80 -0.3 123,199 - GFF2076 0.80 -1.2 123,199 - GFF2076 0.80 -0.0 123,219 + GFF2076 0.81 -1.0 123,220 - GFF2076 0.81 -1.2 123,220 - GFF2076 0.81 +0.3 123,220 - GFF2076 0.81 -1.2 123,220 - GFF2076 0.81 -0.2 123,220 - GFF2076 0.81 -0.7 123,527 + -2.6 123,527 + -1.4 123,545 + +0.1 123,545 + -0.6 123,547 + +0.3 123,617 + GFF2077 0.12 +1.2 123,617 + GFF2077 0.12 +0.4 123,617 + GFF2077 0.12 -0.7 123,617 + GFF2077 0.12 +1.5 123,617 + GFF2077 0.12 -0.2 123,617 + GFF2077 0.12 -2.1 123,618 - GFF2077 0.12 -0.6 123,677 + GFF2077 0.17 -0.3 123,677 + GFF2077 0.17 +0.2 123,707 + GFF2077 0.19 +0.4 123,707 + GFF2077 0.19 -0.2 123,707 + GFF2077 0.19 -0.9 123,707 + GFF2077 0.19 -0.2 123,707 + GFF2077 0.19 -0.6 123,707 + GFF2077 0.19 +0.6 123,708 - GFF2077 0.19 +0.7 123,708 - GFF2077 0.19 +0.4 123,722 + GFF2077 0.20 -0.8 123,722 + GFF2077 0.20 +0.5 123,722 + GFF2077 0.20 +0.8 123,722 + GFF2077 0.20 -0.2 123,722 + GFF2077 0.20 +0.9 123,723 - GFF2077 0.20 +0.0 123,723 - GFF2077 0.20 +0.4 123,723 - GFF2077 0.20 -0.6 123,723 - GFF2077 0.20 +2.0 123,723 - GFF2077 0.20 -0.2 123,723 - GFF2077 0.20 -0.6 123,723 - GFF2077 0.20 -0.4 123,785 + GFF2077 0.25 -0.5 123,785 + GFF2077 0.25 -0.8 123,785 + GFF2077 0.25 -0.7 123,785 + GFF2077 0.25 +1.2 123,786 - GFF2077 0.25 -1.0 123,872 + GFF2077 0.31 +0.2 123,872 + GFF2077 0.31 +0.4 123,873 - GFF2077 0.31 -1.0 123,873 - GFF2077 0.31 +1.0
Or see this region's nucleotide sequence