Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2066 and GFF2067 are separated by 7 nucleotides GFF2067 and GFF2068 overlap by 4 nucleotides GFF2068 and GFF2069 are separated by 152 nucleotides
GFF2066 - Tyrosyl-tRNA synthetase (EC 6.1.1.1), at 114,434 to 115,642
GFF2066
GFF2067 - OmpA/MotB, at 115,650 to 116,273
GFF2067
GFF2068 - hypothetical protein, at 116,270 to 116,734
GFF2068
GFF2069 - Cobalt-zinc-cadmium resistance protein, at 116,887 to 117,804
GFF2069
Position (kb)
115
116
117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.960 kb on + strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.961 kb on - strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.969 kb on + strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 115.970 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.093 kb on - strand, within GFF2067 at 116.776 kb on + strand at 116.776 kb on + strand at 116.776 kb on + strand at 116.777 kb on - strand at 116.777 kb on - strand at 116.777 kb on - strand at 116.780 kb on - strand at 116.793 kb on + strand at 116.793 kb on + strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.794 kb on - strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.797 kb on + strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.798 kb on - strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.837 kb on + strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.838 kb on - strand at 116.840 kb on + strand at 116.841 kb on - strand at 116.967 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.968 kb on + strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 116.969 kb on - strand at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.112 kb on + strand, within GFF2069 at 117.113 kb on - strand, within GFF2069 at 117.113 kb on - strand, within GFF2069 at 117.113 kb on - strand, within GFF2069
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 115,960 + GFF2067 0.50 -0.1 115,960 + GFF2067 0.50 -1.6 115,960 + GFF2067 0.50 +0.1 115,960 + GFF2067 0.50 -1.1 115,960 + GFF2067 0.50 -0.7 115,961 - GFF2067 0.50 -1.1 115,961 - GFF2067 0.50 -0.4 115,961 - GFF2067 0.50 -0.5 115,961 - GFF2067 0.50 -0.3 115,961 - GFF2067 0.50 +0.4 115,961 - GFF2067 0.50 +0.3 115,961 - GFF2067 0.50 -0.5 115,961 - GFF2067 0.50 -0.6 115,961 - GFF2067 0.50 +1.4 115,961 - GFF2067 0.50 -1.0 115,961 - GFF2067 0.50 -0.7 115,961 - GFF2067 0.50 -1.0 115,961 - GFF2067 0.50 -0.8 115,969 + GFF2067 0.51 -0.5 115,969 + GFF2067 0.51 -0.4 115,969 + GFF2067 0.51 -0.9 115,969 + GFF2067 0.51 -0.4 115,970 - GFF2067 0.51 +0.9 115,970 - GFF2067 0.51 -0.2 115,970 - GFF2067 0.51 +1.6 115,970 - GFF2067 0.51 +0.7 116,093 - GFF2067 0.71 +0.2 116,093 - GFF2067 0.71 +0.2 116,093 - GFF2067 0.71 -0.6 116,093 - GFF2067 0.71 +0.1 116,776 + -0.7 116,776 + -0.6 116,776 + -0.4 116,777 - +0.4 116,777 - -0.4 116,777 - -0.6 116,780 - +0.0 116,793 + -0.0 116,793 + +0.0 116,794 - -1.9 116,794 - -0.5 116,794 - -0.2 116,794 - -0.1 116,797 + -0.6 116,797 + -0.0 116,797 + -0.5 116,797 + -0.7 116,797 + -0.6 116,798 - -0.7 116,798 - -0.5 116,798 - +0.1 116,798 - +0.7 116,837 + -0.3 116,837 + -0.2 116,837 + +0.2 116,837 + -0.0 116,837 + -0.4 116,837 + +1.9 116,837 + -0.4 116,837 + -0.1 116,837 + +1.5 116,837 + +0.9 116,837 + +0.4 116,837 + +2.2 116,837 + -0.2 116,838 - +0.2 116,838 - +0.3 116,838 - +1.1 116,838 - -1.2 116,838 - -0.2 116,838 - +0.1 116,838 - -0.8 116,838 - -0.4 116,838 - -0.0 116,838 - +0.0 116,838 - -2.3 116,840 + -0.6 116,841 - +0.4 116,967 + -0.3 116,968 + -1.0 116,968 + +0.0 116,968 + -0.3 116,968 + -1.2 116,968 + -0.7 116,969 - +0.3 116,969 - +0.6 116,969 - -0.2 116,969 - -0.0 116,969 - -0.3 116,969 - -3.1 117,112 + GFF2069 0.25 +0.0 117,112 + GFF2069 0.25 -0.4 117,112 + GFF2069 0.25 -0.1 117,112 + GFF2069 0.25 -1.1 117,112 + GFF2069 0.25 -0.2 117,112 + GFF2069 0.25 +0.2 117,112 + GFF2069 0.25 -0.2 117,112 + GFF2069 0.25 +1.0 117,112 + GFF2069 0.25 +1.0 117,112 + GFF2069 0.25 -0.8 117,113 - GFF2069 0.25 -0.2 117,113 - GFF2069 0.25 +0.9 117,113 - GFF2069 0.25 -0.4
Or see this region's nucleotide sequence