Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1966 and GFF1967 are separated by 3 nucleotides GFF1967 and GFF1968 are separated by 8 nucleotides GFF1968 and GFF1969 overlap by 4 nucleotides
GFF1966 - Apolipoprotein N-acyltransferase / Copper homeostasis protein CutE, at 8,226 to 9,800
GFF1966
GFF1967 - FAD linked oxidase-like, at 9,804 to 11,180
GFF1967
GFF1968 - Hydroxymethylpyrimidine ABC transporter, transmembrane component, at 11,189 to 11,995
GFF1968
GFF1969 - ABC transporter, ATP-binding protein (cluster 1, maltose/g3p/polyamine/iron); ABC transporter, ATP-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 11,992 to 12,828
GFF1969
Position (kb)
9
10
11
12 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 9.132 kb on + strand, within GFF1966 at 9.133 kb on - strand, within GFF1966 at 9.133 kb on - strand, within GFF1966 at 9.133 kb on - strand, within GFF1966 at 9.133 kb on - strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.189 kb on + strand, within GFF1966 at 9.190 kb on - strand, within GFF1966 at 9.190 kb on - strand, within GFF1966 at 9.190 kb on - strand, within GFF1966 at 9.190 kb on - strand, within GFF1966 at 9.190 kb on - strand, within GFF1966 at 9.234 kb on + strand, within GFF1966 at 9.234 kb on + strand, within GFF1966 at 9.234 kb on + strand, within GFF1966 at 9.234 kb on + strand, within GFF1966 at 9.234 kb on + strand, within GFF1966 at 9.241 kb on - strand, within GFF1966 at 9.241 kb on - strand, within GFF1966 at 9.400 kb on - strand, within GFF1966 at 9.693 kb on + strand at 9.694 kb on - strand at 9.694 kb on - strand at 10.171 kb on + strand, within GFF1967 at 10.171 kb on + strand, within GFF1967 at 10.171 kb on + strand, within GFF1967 at 10.171 kb on + strand, within GFF1967 at 10.171 kb on + strand, within GFF1967 at 10.172 kb on - strand, within GFF1967 at 10.172 kb on - strand, within GFF1967 at 10.690 kb on + strand, within GFF1967 at 10.691 kb on - strand, within GFF1967 at 10.691 kb on - strand, within GFF1967 at 10.753 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.873 kb on + strand, within GFF1967 at 10.874 kb on - strand, within GFF1967 at 10.874 kb on - strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.924 kb on + strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 10.925 kb on - strand, within GFF1967 at 11.346 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.448 kb on + strand, within GFF1968 at 11.449 kb on - strand, within GFF1968 at 11.517 kb on + strand, within GFF1968 at 11.517 kb on + strand, within GFF1968 at 11.518 kb on - strand, within GFF1968 at 11.518 kb on - strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.853 kb on + strand, within GFF1968 at 11.871 kb on + strand, within GFF1968 at 11.871 kb on + strand, within GFF1968 at 11.944 kb on - strand at 12.102 kb on - strand, within GFF1969 at 12.130 kb on + strand, within GFF1969
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 9,132 + GFF1966 0.58 -1.1 9,133 - GFF1966 0.58 -1.2 9,133 - GFF1966 0.58 -1.4 9,133 - GFF1966 0.58 -0.2 9,133 - GFF1966 0.58 -1.2 9,189 + GFF1966 0.61 +0.2 9,189 + GFF1966 0.61 -1.0 9,189 + GFF1966 0.61 +1.8 9,189 + GFF1966 0.61 -0.2 9,189 + GFF1966 0.61 -0.9 9,189 + GFF1966 0.61 -1.2 9,190 - GFF1966 0.61 +1.2 9,190 - GFF1966 0.61 -2.6 9,190 - GFF1966 0.61 -0.8 9,190 - GFF1966 0.61 -0.6 9,190 - GFF1966 0.61 -0.1 9,234 + GFF1966 0.64 -1.3 9,234 + GFF1966 0.64 -1.8 9,234 + GFF1966 0.64 -0.5 9,234 + GFF1966 0.64 -1.2 9,234 + GFF1966 0.64 -0.5 9,241 - GFF1966 0.64 -1.2 9,241 - GFF1966 0.64 +0.0 9,400 - GFF1966 0.75 +0.1 9,693 + -0.5 9,694 - -0.6 9,694 - +0.0 10,171 + GFF1967 0.27 -0.5 10,171 + GFF1967 0.27 -0.3 10,171 + GFF1967 0.27 -0.0 10,171 + GFF1967 0.27 -0.3 10,171 + GFF1967 0.27 +1.3 10,172 - GFF1967 0.27 +0.5 10,172 - GFF1967 0.27 -0.8 10,690 + GFF1967 0.64 +1.2 10,691 - GFF1967 0.64 -0.8 10,691 - GFF1967 0.64 -0.8 10,753 + GFF1967 0.69 +0.2 10,873 + GFF1967 0.78 -1.0 10,873 + GFF1967 0.78 +1.4 10,873 + GFF1967 0.78 +0.7 10,873 + GFF1967 0.78 +0.2 10,874 - GFF1967 0.78 -1.3 10,874 - GFF1967 0.78 +0.8 10,924 + GFF1967 0.81 -0.7 10,924 + GFF1967 0.81 -0.5 10,924 + GFF1967 0.81 +0.8 10,924 + GFF1967 0.81 -0.5 10,925 - GFF1967 0.81 +0.5 10,925 - GFF1967 0.81 -1.4 10,925 - GFF1967 0.81 +0.3 11,346 + GFF1968 0.19 +1.2 11,448 + GFF1968 0.32 +1.4 11,448 + GFF1968 0.32 -0.8 11,448 + GFF1968 0.32 -0.4 11,448 + GFF1968 0.32 +1.1 11,448 + GFF1968 0.32 -0.4 11,449 - GFF1968 0.32 -0.1 11,517 + GFF1968 0.41 -0.6 11,517 + GFF1968 0.41 -0.5 11,518 - GFF1968 0.41 +0.2 11,518 - GFF1968 0.41 -0.1 11,853 + GFF1968 0.82 -1.0 11,853 + GFF1968 0.82 -0.5 11,853 + GFF1968 0.82 -0.8 11,853 + GFF1968 0.82 +0.5 11,871 + GFF1968 0.85 -0.3 11,871 + GFF1968 0.85 -0.8 11,944 - +0.4 12,102 - GFF1969 0.13 -0.7 12,130 + GFF1969 0.16 -0.6
Or see this region's nucleotide sequence