Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1933 and GFF1934 are separated by 95 nucleotides GFF1934 and GFF1935 are separated by 172 nucleotides GFF1935 and GFF1936 are separated by 34 nucleotides
GFF1933 - Alkaline phosphatase (EC 3.1.3.1), at 615,536 to 627,136
GFF1933
GFF1934 - Transcriptional regulator, GntR family domain / Aspartate aminotransferase (EC 2.6.1.1), at 627,232 to 628,638
GFF1934
GFF1935 - GNAT family acetyltransferase PA5433, at 628,811 to 629,524
GFF1935
GFF1936 - Bis(5'-nucleosyl)-tetraphosphatase, symmetrical (EC 3.6.1.41), at 629,559 to 630,404
GFF1936
Position (kb)
627
628
629 Strain fitness (log2 ratio)
-2
-1
0
1 at 626.268 kb on - strand at 626.268 kb on - strand at 626.297 kb on - strand at 626.346 kb on - strand at 626.448 kb on - strand at 626.492 kb on + strand at 626.499 kb on + strand at 626.520 kb on + strand at 626.609 kb on + strand at 626.610 kb on - strand at 626.610 kb on - strand at 626.664 kb on - strand at 626.711 kb on - strand at 626.739 kb on + strand at 626.741 kb on + strand at 626.741 kb on + strand at 626.811 kb on + strand at 626.933 kb on + strand at 626.934 kb on - strand at 626.935 kb on + strand at 626.936 kb on - strand at 626.936 kb on - strand at 626.936 kb on - strand at 626.936 kb on - strand at 626.971 kb on + strand at 627.115 kb on + strand at 627.211 kb on + strand at 627.211 kb on + strand at 627.211 kb on + strand at 627.225 kb on + strand at 627.225 kb on + strand at 627.226 kb on - strand at 627.226 kb on - strand at 627.306 kb on + strand at 627.306 kb on + strand at 627.902 kb on + strand, within GFF1934 at 627.923 kb on + strand, within GFF1934 at 628.227 kb on - strand, within GFF1934 at 628.481 kb on + strand, within GFF1934 at 628.482 kb on - strand, within GFF1934 at 628.505 kb on + strand at 628.505 kb on + strand at 628.506 kb on - strand at 628.506 kb on - strand at 628.506 kb on - strand at 628.758 kb on + strand at 628.759 kb on - strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.763 kb on + strand at 628.764 kb on - strand at 628.764 kb on - strand at 628.764 kb on - strand at 629.000 kb on + strand, within GFF1935 at 629.001 kb on - strand, within GFF1935 at 629.001 kb on - strand, within GFF1935 at 629.046 kb on - strand, within GFF1935 at 629.046 kb on - strand, within GFF1935 at 629.228 kb on + strand, within GFF1935 at 629.339 kb on + strand, within GFF1935 at 629.339 kb on + strand, within GFF1935 at 629.339 kb on + strand, within GFF1935 at 629.340 kb on - strand, within GFF1935 at 629.369 kb on + strand, within GFF1935 at 629.369 kb on + strand, within GFF1935 at 629.370 kb on - strand, within GFF1935 at 629.370 kb on - strand, within GFF1935 at 629.561 kb on - strand at 629.561 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 626,268 - -0.2 626,268 - +0.5 626,297 - +1.2 626,346 - -0.3 626,448 - -0.3 626,492 + +1.1 626,499 + -0.8 626,520 + -0.3 626,609 + +0.1 626,610 - -0.6 626,610 - +0.2 626,664 - +0.3 626,711 - -1.3 626,739 + -0.3 626,741 + -0.1 626,741 + -0.8 626,811 + -0.3 626,933 + -0.6 626,934 - +0.9 626,935 + +0.7 626,936 - -2.5 626,936 - -2.5 626,936 - +0.3 626,936 - +0.4 626,971 + -0.6 627,115 + -0.7 627,211 + +0.2 627,211 + +0.3 627,211 + +0.1 627,225 + -1.3 627,225 + -1.1 627,226 - -0.1 627,226 - -1.3 627,306 + -0.2 627,306 + -0.8 627,902 + GFF1934 0.48 +0.3 627,923 + GFF1934 0.49 -0.2 628,227 - GFF1934 0.71 -0.1 628,481 + GFF1934 0.89 +0.2 628,482 - GFF1934 0.89 +0.8 628,505 + -0.5 628,505 + -0.8 628,506 - +0.5 628,506 - -0.2 628,506 - -0.8 628,758 + -0.8 628,759 - +0.0 628,763 + +1.3 628,763 + -0.5 628,763 + +0.1 628,763 + -0.1 628,763 + -0.1 628,763 + +0.3 628,763 + +0.2 628,763 + -0.8 628,763 + -0.7 628,763 + -0.8 628,763 + -0.1 628,764 - +0.4 628,764 - +0.2 628,764 - +0.6 629,000 + GFF1935 0.26 +0.1 629,001 - GFF1935 0.27 +0.6 629,001 - GFF1935 0.27 -0.1 629,046 - GFF1935 0.33 +0.3 629,046 - GFF1935 0.33 -1.3 629,228 + GFF1935 0.58 -0.4 629,339 + GFF1935 0.74 +0.1 629,339 + GFF1935 0.74 -1.0 629,339 + GFF1935 0.74 +0.2 629,340 - GFF1935 0.74 +1.5 629,369 + GFF1935 0.78 -0.5 629,369 + GFF1935 0.78 +1.0 629,370 - GFF1935 0.78 +1.0 629,370 - GFF1935 0.78 +0.0 629,561 - -0.2 629,561 - +0.4
Or see this region's nucleotide sequence