Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1879 and GFF1880 are separated by 279 nucleotides GFF1880 and GFF1881 overlap by 4 nucleotides
GFF1879 - dicarboxylic acid transporter PcaT, at 551,755 to 553,101
GFF1879
GFF1880 - Previously called glutamate synthase [NADPH] small chain, at 553,381 to 555,180
GFF1880
GFF1881 - 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, gamma subunit (EC 1.2.7.-) / 2-oxoglutarate/2-oxoacid ferredoxin oxidoreductase, alpha subunit (EC 1.2.7.-), at 555,177 to 557,003
GFF1881
Position (kb) 553
554
555
556 Strain fitness (log2 ratio) -2
-1
0
1
2 at 552.566 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.620 kb on + strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.621 kb on - strand, within GFF1879 at 552.717 kb on - strand, within GFF1879 at 552.717 kb on - strand, within GFF1879 at 552.923 kb on + strand, within GFF1879 at 552.923 kb on + strand, within GFF1879 at 552.923 kb on + strand, within GFF1879 at 552.923 kb on + strand, within GFF1879 at 552.924 kb on - strand, within GFF1879 at 552.924 kb on - strand, within GFF1879 at 552.941 kb on + strand, within GFF1879 at 552.947 kb on + strand, within GFF1879 at 552.948 kb on - strand, within GFF1879 at 552.948 kb on - strand, within GFF1879 at 552.948 kb on - strand, within GFF1879 at 552.948 kb on - strand, within GFF1879 at 553.028 kb on + strand at 553.028 kb on + strand at 553.029 kb on - strand at 553.029 kb on - strand at 553.029 kb on - strand at 553.029 kb on - strand at 553.029 kb on - strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.214 kb on + strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.215 kb on - strand at 553.225 kb on - strand at 553.242 kb on + strand at 553.242 kb on + strand at 553.243 kb on - strand at 553.318 kb on + strand at 553.319 kb on - strand at 553.319 kb on - strand at 553.375 kb on + strand at 553.375 kb on + strand at 553.375 kb on + strand at 553.375 kb on + strand at 553.376 kb on - strand at 553.376 kb on - strand at 553.411 kb on + strand at 553.412 kb on - strand at 553.412 kb on - strand at 553.439 kb on - strand at 553.471 kb on + strand at 553.471 kb on + strand at 553.472 kb on - strand at 553.472 kb on - strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.475 kb on + strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.476 kb on - strand at 553.498 kb on + strand at 553.510 kb on + strand at 553.510 kb on + strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.511 kb on - strand at 553.894 kb on + strand, within GFF1880 at 553.894 kb on + strand, within GFF1880 at 553.894 kb on + strand, within GFF1880 at 553.894 kb on + strand, within GFF1880 at 553.895 kb on - strand, within GFF1880 at 553.895 kb on - strand, within GFF1880 at 554.356 kb on + strand, within GFF1880 at 554.356 kb on + strand, within GFF1880 at 554.357 kb on - strand, within GFF1880 at 554.659 kb on + strand, within GFF1880 at 554.660 kb on - strand, within GFF1880 at 554.660 kb on - strand, within GFF1880 at 554.722 kb on + strand, within GFF1880 at 554.723 kb on - strand, within GFF1880 at 554.752 kb on + strand, within GFF1880 at 554.752 kb on + strand, within GFF1880 at 554.752 kb on + strand, within GFF1880 at 554.753 kb on - strand, within GFF1880 at 554.753 kb on - strand, within GFF1880 at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.022 kb on + strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.023 kb on - strand at 555.678 kb on + strand, within GFF1881 at 555.678 kb on + strand, within GFF1881 at 555.678 kb on + strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.679 kb on - strand, within GFF1881 at 555.849 kb on + strand, within GFF1881 at 555.850 kb on - strand, within GFF1881 at 555.850 kb on - strand, within GFF1881 at 555.876 kb on + strand, within GFF1881 at 555.876 kb on + strand, within GFF1881 at 555.921 kb on + strand, within GFF1881 at 555.922 kb on - strand, within GFF1881 at 555.922 kb on - strand, within GFF1881 at 556.101 kb on + strand, within GFF1881 at 556.101 kb on + strand, within GFF1881 at 556.102 kb on - strand, within GFF1881
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 552,566 + GFF1879 0.60 +1.6 552,620 + GFF1879 0.64 -0.5 552,620 + GFF1879 0.64 -0.5 552,620 + GFF1879 0.64 +0.1 552,620 + GFF1879 0.64 +2.1 552,620 + GFF1879 0.64 -1.8 552,620 + GFF1879 0.64 -0.2 552,620 + GFF1879 0.64 -0.6 552,621 - GFF1879 0.64 -0.4 552,621 - GFF1879 0.64 -0.4 552,621 - GFF1879 0.64 -0.8 552,621 - GFF1879 0.64 -1.0 552,621 - GFF1879 0.64 -0.3 552,717 - GFF1879 0.71 -1.2 552,717 - GFF1879 0.71 -0.1 552,923 + GFF1879 0.87 +0.1 552,923 + GFF1879 0.87 -0.0 552,923 + GFF1879 0.87 -0.3 552,923 + GFF1879 0.87 -0.6 552,924 - GFF1879 0.87 +0.2 552,924 - GFF1879 0.87 -0.0 552,941 + GFF1879 0.88 +0.2 552,947 + GFF1879 0.88 -1.1 552,948 - GFF1879 0.89 -0.7 552,948 - GFF1879 0.89 -0.9 552,948 - GFF1879 0.89 -0.2 552,948 - GFF1879 0.89 +0.7 553,028 + +0.5 553,028 + -0.2 553,029 - -0.5 553,029 - -0.5 553,029 - -0.3 553,029 - +0.7 553,029 - -1.0 553,214 + -1.0 553,214 + -1.1 553,214 + -0.5 553,214 + +0.3 553,214 + -0.7 553,214 + +0.4 553,214 + +0.3 553,214 + +0.3 553,214 + -1.3 553,214 + +0.3 553,214 + -0.3 553,214 + -0.3 553,215 - +2.5 553,215 - -0.3 553,215 - -0.2 553,215 - -1.0 553,215 - +1.3 553,215 - -0.2 553,215 - +0.5 553,225 - +1.1 553,242 + -0.9 553,242 + -0.4 553,243 - -0.8 553,318 + +0.2 553,319 - -0.4 553,319 - -0.7 553,375 + +0.1 553,375 + -0.1 553,375 + +0.7 553,375 + +0.5 553,376 - -0.1 553,376 - +0.4 553,411 + +0.3 553,412 - +0.2 553,412 - +1.3 553,439 - -0.3 553,471 + +1.7 553,471 + +1.2 553,472 - +0.2 553,472 - +0.0 553,475 + +0.2 553,475 + +0.0 553,475 + +0.4 553,475 + +0.3 553,475 + +0.4 553,475 + +0.8 553,475 + -0.8 553,475 + -1.4 553,475 + -0.7 553,475 + -1.0 553,475 + +0.6 553,476 - -0.5 553,476 - -1.1 553,476 - +0.6 553,476 - -0.1 553,476 - -1.3 553,476 - +0.4 553,476 - -0.0 553,476 - -0.4 553,498 + +0.2 553,510 + -0.4 553,510 + +0.0 553,511 - -1.3 553,511 - -0.6 553,511 - -0.2 553,511 - -1.4 553,511 - -1.1 553,511 - -1.4 553,511 - -1.7 553,511 - +0.8 553,511 - +0.4 553,511 - -0.7 553,894 + GFF1880 0.28 -0.4 553,894 + GFF1880 0.28 -0.1 553,894 + GFF1880 0.28 +1.7 553,894 + GFF1880 0.28 -0.3 553,895 - GFF1880 0.29 -1.1 553,895 - GFF1880 0.29 -0.1 554,356 + GFF1880 0.54 -1.1 554,356 + GFF1880 0.54 -0.3 554,357 - GFF1880 0.54 -0.1 554,659 + GFF1880 0.71 +0.6 554,660 - GFF1880 0.71 +1.1 554,660 - GFF1880 0.71 -0.2 554,722 + GFF1880 0.74 -1.3 554,723 - GFF1880 0.75 +0.2 554,752 + GFF1880 0.76 +0.0 554,752 + GFF1880 0.76 -2.0 554,752 + GFF1880 0.76 +2.2 554,753 - GFF1880 0.76 -0.5 554,753 - GFF1880 0.76 +1.1 555,022 + -1.0 555,022 + -0.6 555,022 + -0.3 555,022 + -0.6 555,022 + +1.0 555,022 + -0.1 555,022 + -0.1 555,022 + +0.3 555,023 - -0.2 555,023 - +1.2 555,023 - -1.0 555,023 - +0.2 555,023 - +0.1 555,023 - -0.7 555,023 - +0.6 555,023 - -0.4 555,023 - -1.2 555,023 - -0.5 555,023 - +0.2 555,678 + GFF1881 0.27 +0.7 555,678 + GFF1881 0.27 +0.7 555,678 + GFF1881 0.27 -0.9 555,679 - GFF1881 0.27 +0.9 555,679 - GFF1881 0.27 +0.7 555,679 - GFF1881 0.27 -0.3 555,679 - GFF1881 0.27 -0.1 555,679 - GFF1881 0.27 -0.6 555,679 - GFF1881 0.27 -1.2 555,679 - GFF1881 0.27 -0.1 555,679 - GFF1881 0.27 -0.2 555,849 + GFF1881 0.37 -0.1 555,850 - GFF1881 0.37 -0.4 555,850 - GFF1881 0.37 +0.7 555,876 + GFF1881 0.38 +0.3 555,876 + GFF1881 0.38 -0.3 555,921 + GFF1881 0.41 -1.4 555,922 - GFF1881 0.41 -0.5 555,922 - GFF1881 0.41 -1.0 556,101 + GFF1881 0.51 -0.3 556,101 + GFF1881 0.51 -0.2 556,102 - GFF1881 0.51 -0.7
Or see this region's nucleotide sequence