Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1816 and GFF1817 are separated by 74 nucleotides GFF1817 and GFF1818 are separated by 4 nucleotides GFF1818 and GFF1819 are separated by 56 nucleotides
GFF1816 - Transcriptional regulator, GntR family, at 480,777 to 481,451
GFF1816
GFF1817 - 5-hydroxyisourate hydrolase (EC 3.5.2.17), at 481,526 to 481,876
GFF1817
GFF1818 - FIG00932412: hypothetical protein, at 481,881 to 482,378
GFF1818
GFF1819 - Xanthine and CO dehydrogenases maturation factor, XdhC/CoxF family, at 482,435 to 483,244
GFF1819
Position (kb) 481
482
483 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 480.958 kb on + strand, within GFF1816 at 480.959 kb on - strand, within GFF1816 at 480.959 kb on - strand, within GFF1816 at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.454 kb on + strand at 481.476 kb on - strand at 481.545 kb on + strand at 481.546 kb on - strand at 481.595 kb on + strand, within GFF1817 at 481.595 kb on + strand, within GFF1817 at 481.596 kb on - strand, within GFF1817 at 481.596 kb on - strand, within GFF1817 at 481.673 kb on + strand, within GFF1817 at 481.673 kb on + strand, within GFF1817 at 481.703 kb on + strand, within GFF1817 at 481.704 kb on - strand, within GFF1817 at 481.730 kb on + strand, within GFF1817 at 481.730 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.820 kb on + strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.821 kb on - strand, within GFF1817 at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.853 kb on + strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.854 kb on - strand at 481.862 kb on + strand at 481.863 kb on - strand at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.152 kb on + strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.153 kb on - strand, within GFF1818 at 482.176 kb on + strand, within GFF1818 at 482.176 kb on + strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.177 kb on - strand, within GFF1818 at 482.363 kb on + strand at 482.363 kb on + strand at 482.364 kb on - strand at 482.388 kb on + strand at 482.388 kb on + strand at 482.424 kb on + strand at 482.720 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.059 kb on + strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819 at 483.060 kb on - strand, within GFF1819
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 480,958 + GFF1816 0.27 +0.9 480,959 - GFF1816 0.27 +0.2 480,959 - GFF1816 0.27 -0.3 481,454 + +0.2 481,454 + -2.0 481,454 + -1.1 481,454 + -0.9 481,454 + -0.9 481,454 + -1.5 481,454 + -0.8 481,476 - -0.1 481,545 + -0.3 481,546 - -0.5 481,595 + GFF1817 0.20 -0.6 481,595 + GFF1817 0.20 +0.2 481,596 - GFF1817 0.20 -0.3 481,596 - GFF1817 0.20 +0.7 481,673 + GFF1817 0.42 +0.5 481,673 + GFF1817 0.42 +0.4 481,703 + GFF1817 0.50 +0.5 481,704 - GFF1817 0.51 +0.5 481,730 + GFF1817 0.58 -0.0 481,730 + GFF1817 0.58 -0.3 481,820 + GFF1817 0.84 -0.3 481,820 + GFF1817 0.84 -0.4 481,820 + GFF1817 0.84 -0.7 481,820 + GFF1817 0.84 -0.3 481,820 + GFF1817 0.84 +0.0 481,820 + GFF1817 0.84 -0.6 481,820 + GFF1817 0.84 -0.9 481,821 - GFF1817 0.84 -0.6 481,821 - GFF1817 0.84 -1.2 481,821 - GFF1817 0.84 -3.0 481,821 - GFF1817 0.84 -1.2 481,821 - GFF1817 0.84 -0.5 481,853 + -1.2 481,853 + +0.4 481,853 + -0.1 481,853 + -0.7 481,853 + -0.4 481,854 - +0.2 481,854 - +1.0 481,854 - -0.8 481,854 - -0.5 481,862 + -0.8 481,863 - -0.6 482,152 + GFF1818 0.54 -0.7 482,152 + GFF1818 0.54 -0.5 482,152 + GFF1818 0.54 -0.9 482,152 + GFF1818 0.54 -0.5 482,152 + GFF1818 0.54 +0.0 482,152 + GFF1818 0.54 +2.3 482,152 + GFF1818 0.54 -0.0 482,153 - GFF1818 0.55 +1.0 482,153 - GFF1818 0.55 -1.1 482,153 - GFF1818 0.55 -0.8 482,153 - GFF1818 0.55 +0.0 482,153 - GFF1818 0.55 -0.2 482,153 - GFF1818 0.55 +1.9 482,176 + GFF1818 0.59 -0.8 482,176 + GFF1818 0.59 -1.7 482,177 - GFF1818 0.59 -0.1 482,177 - GFF1818 0.59 -1.4 482,177 - GFF1818 0.59 -0.1 482,177 - GFF1818 0.59 -1.0 482,177 - GFF1818 0.59 -0.4 482,177 - GFF1818 0.59 -0.3 482,363 + -0.6 482,363 + -0.9 482,364 - -0.0 482,388 + +1.7 482,388 + +0.0 482,424 + -0.7 482,720 + GFF1819 0.35 -1.4 483,059 + GFF1819 0.77 -1.2 483,059 + GFF1819 0.77 +0.1 483,059 + GFF1819 0.77 -0.8 483,059 + GFF1819 0.77 -0.1 483,060 - GFF1819 0.77 -0.2 483,060 - GFF1819 0.77 +0.2 483,060 - GFF1819 0.77 +0.9 483,060 - GFF1819 0.77 +1.3 483,060 - GFF1819 0.77 -0.8 483,060 - GFF1819 0.77 +0.2 483,060 - GFF1819 0.77 +0.2
Or see this region's nucleotide sequence