Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1675 and GFF1676 overlap by 4 nucleotides GFF1676 and GFF1677 are separated by 36 nucleotides GFF1677 and GFF1678 are separated by 17 nucleotides GFF1678 and GFF1679 are separated by 123 nucleotides
GFF1675 - ABC transporter, permease protein 2 (cluster 4, leucine/isoleucine/valine/benzoate), at 320,317 to 321,297
GFF1675
GFF1676 - ABC transporter, permease protein 1 (cluster 4, leucine/isoleucine/valine/benzoate), at 321,294 to 322,166
GFF1676
GFF1677 - ABC transporter, substrate-binding protein (cluster 4, leucine/isoleucine/valine/benzoate), at 322,203 to 323,405
GFF1677
GFF1678 - Transcriptional regulator, GntR family, at 323,423 to 324,214
GFF1678
GFF1679 - Riboflavin synthase eubacterial/eukaryotic (EC 2.5.1.9), at 324,338 to 325,042
GFF1679
Position (kb) 322
323
324 Strain fitness (log2 ratio) -2
-1
0
1
2 at 321.523 kb on + strand, within GFF1676 at 321.523 kb on + strand, within GFF1676 at 321.524 kb on - strand, within GFF1676 at 321.526 kb on + strand, within GFF1676 at 321.526 kb on + strand, within GFF1676 at 321.592 kb on + strand, within GFF1676 at 321.928 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.973 kb on + strand, within GFF1676 at 321.974 kb on - strand, within GFF1676 at 322.007 kb on - strand, within GFF1676 at 322.072 kb on + strand, within GFF1676 at 322.072 kb on + strand, within GFF1676 at 322.073 kb on - strand, within GFF1676 at 322.096 kb on - strand at 322.105 kb on + strand at 322.105 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.161 kb on + strand at 322.162 kb on - strand at 322.339 kb on + strand, within GFF1677 at 322.450 kb on + strand, within GFF1677 at 322.450 kb on + strand, within GFF1677 at 322.450 kb on + strand, within GFF1677 at 322.451 kb on - strand, within GFF1677 at 322.597 kb on + strand, within GFF1677 at 322.597 kb on + strand, within GFF1677 at 322.691 kb on - strand, within GFF1677 at 322.691 kb on - strand, within GFF1677 at 322.693 kb on + strand, within GFF1677 at 322.694 kb on - strand, within GFF1677 at 322.694 kb on - strand, within GFF1677 at 322.952 kb on - strand, within GFF1677 at 323.038 kb on + strand, within GFF1677 at 323.502 kb on + strand at 323.502 kb on + strand at 323.503 kb on - strand, within GFF1678 at 323.565 kb on + strand, within GFF1678 at 323.565 kb on + strand, within GFF1678 at 323.763 kb on + strand, within GFF1678 at 323.763 kb on + strand, within GFF1678 at 323.764 kb on - strand, within GFF1678 at 323.764 kb on - strand, within GFF1678 at 324.177 kb on + strand at 324.178 kb on - strand at 324.190 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 321,523 + GFF1676 0.26 +1.9 321,523 + GFF1676 0.26 -0.3 321,524 - GFF1676 0.26 +1.4 321,526 + GFF1676 0.27 +0.2 321,526 + GFF1676 0.27 +1.2 321,592 + GFF1676 0.34 -0.1 321,928 + GFF1676 0.73 -0.5 321,973 + GFF1676 0.78 -0.1 321,973 + GFF1676 0.78 -0.3 321,973 + GFF1676 0.78 +1.5 321,973 + GFF1676 0.78 +0.4 321,973 + GFF1676 0.78 -0.4 321,974 - GFF1676 0.78 +0.7 322,007 - GFF1676 0.82 +0.1 322,072 + GFF1676 0.89 +2.7 322,072 + GFF1676 0.89 -0.5 322,073 - GFF1676 0.89 -1.2 322,096 - -1.1 322,105 + -1.3 322,105 + -0.2 322,161 + -0.5 322,161 + +0.2 322,161 + -0.3 322,161 + -0.3 322,162 - -0.0 322,339 + GFF1677 0.11 +0.2 322,450 + GFF1677 0.21 -2.1 322,450 + GFF1677 0.21 -0.3 322,450 + GFF1677 0.21 +0.0 322,451 - GFF1677 0.21 +0.1 322,597 + GFF1677 0.33 -0.5 322,597 + GFF1677 0.33 -0.4 322,691 - GFF1677 0.41 -0.7 322,691 - GFF1677 0.41 -1.5 322,693 + GFF1677 0.41 +0.4 322,694 - GFF1677 0.41 -1.6 322,694 - GFF1677 0.41 -0.8 322,952 - GFF1677 0.62 +0.3 323,038 + GFF1677 0.69 +0.6 323,502 + -0.3 323,502 + -0.6 323,503 - GFF1678 0.10 -0.7 323,565 + GFF1678 0.18 +0.7 323,565 + GFF1678 0.18 +0.0 323,763 + GFF1678 0.43 +0.3 323,763 + GFF1678 0.43 -1.6 323,764 - GFF1678 0.43 -0.3 323,764 - GFF1678 0.43 -1.1 324,177 + +0.1 324,178 - +0.1 324,190 - -0.1
Or see this region's nucleotide sequence