Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1612 and GFF1613 are separated by 43 nucleotides GFF1613 and GFF1614 are separated by 2 nucleotides GFF1614 and GFF1615 overlap by 4 nucleotides GFF1615 and GFF1616 are separated by 128 nucleotides
GFF1612 - no description, at 210,819 to 211,112
GFF1612
GFF1613 - Phosphonate ABC transporter permease protein PhnE (TC 3.A.1.9.1), at 211,156 to 211,980
GFF1613
GFF1614 - Phosphonate ABC transporter ATP-binding protein PhnC (TC 3.A.1.9.1), at 211,983 to 212,783
GFF1614
GFF1615 - Phosphonate ABC transporter substrate-binding protein PhnD (TC 3.A.1.9.1), at 212,780 to 213,649
GFF1615
GFF1616 - Phosphonate uptake and metabolism regulator, LysR-family, at 213,778 to 214,665
GFF1616
Position (kb) 211
212
213 Strain fitness (log2 ratio) -2
-1
0
1 at 211.023 kb on + strand, within GFF1612 at 211.024 kb on - strand, within GFF1612 at 211.024 kb on - strand, within GFF1612 at 211.024 kb on - strand, within GFF1612 at 211.024 kb on - strand, within GFF1612 at 211.024 kb on - strand, within GFF1612 at 211.033 kb on + strand, within GFF1612 at 211.033 kb on + strand, within GFF1612 at 211.034 kb on - strand, within GFF1612 at 211.034 kb on - strand, within GFF1612 at 211.034 kb on - strand, within GFF1612 at 211.034 kb on - strand, within GFF1612 at 211.034 kb on - strand, within GFF1612 at 211.035 kb on + strand, within GFF1612 at 211.035 kb on + strand, within GFF1612 at 211.036 kb on - strand, within GFF1612 at 211.041 kb on + strand, within GFF1612 at 211.041 kb on + strand, within GFF1612 at 211.042 kb on - strand, within GFF1612 at 211.042 kb on - strand, within GFF1612 at 211.231 kb on - strand at 211.236 kb on + strand at 211.237 kb on - strand at 211.361 kb on + strand, within GFF1613 at 211.640 kb on + strand, within GFF1613 at 211.640 kb on + strand, within GFF1613 at 211.641 kb on - strand, within GFF1613 at 211.641 kb on - strand, within GFF1613 at 211.641 kb on - strand, within GFF1613 at 211.641 kb on - strand, within GFF1613 at 211.958 kb on + strand at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.069 kb on - strand, within GFF1614 at 212.233 kb on + strand, within GFF1614 at 212.234 kb on - strand, within GFF1614 at 212.234 kb on - strand, within GFF1614 at 212.774 kb on + strand at 212.838 kb on + strand at 212.839 kb on - strand at 212.839 kb on - strand at 213.300 kb on + strand, within GFF1615 at 213.375 kb on + strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.376 kb on - strand, within GFF1615 at 213.441 kb on + strand, within GFF1615 at 213.716 kb on + strand at 213.716 kb on + strand at 213.717 kb on - strand at 213.717 kb on - strand at 213.729 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.773 kb on + strand at 213.774 kb on - strand at 213.774 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 211,023 + GFF1612 0.69 -2.1 211,024 - GFF1612 0.70 +0.3 211,024 - GFF1612 0.70 -0.0 211,024 - GFF1612 0.70 +0.7 211,024 - GFF1612 0.70 +1.0 211,024 - GFF1612 0.70 -0.1 211,033 + GFF1612 0.73 -0.3 211,033 + GFF1612 0.73 +0.3 211,034 - GFF1612 0.73 +0.9 211,034 - GFF1612 0.73 -0.8 211,034 - GFF1612 0.73 -0.7 211,034 - GFF1612 0.73 -0.4 211,034 - GFF1612 0.73 -1.4 211,035 + GFF1612 0.73 -0.2 211,035 + GFF1612 0.73 -0.5 211,036 - GFF1612 0.74 -0.1 211,041 + GFF1612 0.76 +0.6 211,041 + GFF1612 0.76 +0.9 211,042 - GFF1612 0.76 +0.1 211,042 - GFF1612 0.76 -2.0 211,231 - -0.8 211,236 + -2.3 211,237 - +0.1 211,361 + GFF1613 0.25 -1.1 211,640 + GFF1613 0.59 -0.2 211,640 + GFF1613 0.59 -0.7 211,641 - GFF1613 0.59 -0.6 211,641 - GFF1613 0.59 +1.7 211,641 - GFF1613 0.59 -0.1 211,641 - GFF1613 0.59 -0.8 211,958 + -0.2 212,069 - GFF1614 0.11 +0.3 212,069 - GFF1614 0.11 -0.6 212,069 - GFF1614 0.11 +0.1 212,069 - GFF1614 0.11 -0.1 212,233 + GFF1614 0.31 +0.3 212,234 - GFF1614 0.31 +0.5 212,234 - GFF1614 0.31 +0.0 212,774 + -0.5 212,838 + +0.4 212,839 - -1.5 212,839 - +0.1 213,300 + GFF1615 0.60 -1.1 213,375 + GFF1615 0.68 +0.9 213,376 - GFF1615 0.69 -0.9 213,376 - GFF1615 0.69 -0.9 213,376 - GFF1615 0.69 -0.2 213,376 - GFF1615 0.69 -1.3 213,376 - GFF1615 0.69 -0.3 213,441 + GFF1615 0.76 +0.1 213,716 + -1.1 213,716 + -0.4 213,717 - -0.3 213,717 - +0.2 213,729 + -0.3 213,773 + +0.2 213,773 + -1.3 213,773 + -0.1 213,773 + -0.2 213,774 - -0.4 213,774 - -0.7
Or see this region's nucleotide sequence