Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1602 and GFF1603 overlap by 4 nucleotides GFF1603 and GFF1604 are separated by 1 nucleotides GFF1604 and GFF1605 are separated by 108 nucleotides GFF1605 and GFF1606 are separated by 3 nucleotides
GFF1602 - Branched-chain amino acid transport ATP-binding protein LivF (TC 3.A.1.4.1), at 200,243 to 200,965
GFF1602
GFF1603 - Branched-chain amino acid ABC transporter, ATP-binding protein LivG (TC 3.A.1.4.1), at 200,962 to 201,729
GFF1603
GFF1604 - Prolidase (EC 3.4.13.9), at 201,731 to 202,996
GFF1604
GFF1605 - no description, at 203,105 to 203,686
GFF1605
GFF1606 - Cys-tRNA(Pro) deacylase YbaK, at 203,690 to 205,276
GFF1606
Position (kb)
201
202
203 Strain fitness (log2 ratio)
-1
0
1 at 200.985 kb on - strand at 200.985 kb on - strand at 201.042 kb on - strand, within GFF1603 at 201.758 kb on + strand at 202.302 kb on + strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.303 kb on - strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.320 kb on + strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.321 kb on - strand, within GFF1604 at 202.432 kb on + strand, within GFF1604 at 202.433 kb on - strand, within GFF1604 at 202.433 kb on - strand, within GFF1604 at 202.851 kb on + strand, within GFF1604 at 202.988 kb on + strand at 203.023 kb on - strand at 203.045 kb on - strand at 203.075 kb on + strand at 203.114 kb on - strand at 203.114 kb on - strand at 203.263 kb on + strand, within GFF1605 at 203.264 kb on - strand, within GFF1605 at 203.584 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.612 kb on + strand, within GFF1605 at 203.613 kb on - strand, within GFF1605 at 203.613 kb on - strand, within GFF1605 at 203.613 kb on - strand, within GFF1605 at 203.636 kb on + strand at 203.710 kb on - strand at 203.710 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 200,985 - +0.0 200,985 - -0.8 201,042 - GFF1603 0.10 -0.5 201,758 + -0.8 202,302 + GFF1604 0.45 -0.3 202,303 - GFF1604 0.45 -0.8 202,303 - GFF1604 0.45 -0.3 202,320 + GFF1604 0.47 +0.3 202,320 + GFF1604 0.47 -0.9 202,320 + GFF1604 0.47 -0.5 202,321 - GFF1604 0.47 -0.5 202,321 - GFF1604 0.47 +0.0 202,321 - GFF1604 0.47 +0.7 202,321 - GFF1604 0.47 -0.3 202,432 + GFF1604 0.55 -0.2 202,433 - GFF1604 0.55 +0.4 202,433 - GFF1604 0.55 -1.3 202,851 + GFF1604 0.88 -1.5 202,988 + -0.7 203,023 - -0.1 203,045 - -0.1 203,075 + +0.2 203,114 - +0.4 203,114 - -0.9 203,263 + GFF1605 0.27 -0.1 203,264 - GFF1605 0.27 -0.3 203,584 + GFF1605 0.82 +1.2 203,612 + GFF1605 0.87 -0.7 203,612 + GFF1605 0.87 -0.3 203,612 + GFF1605 0.87 -1.7 203,612 + GFF1605 0.87 -0.4 203,612 + GFF1605 0.87 -0.5 203,612 + GFF1605 0.87 -0.2 203,613 - GFF1605 0.87 +0.0 203,613 - GFF1605 0.87 -0.1 203,613 - GFF1605 0.87 -1.1 203,636 + -0.2 203,710 - -0.6 203,710 - -1.4
Or see this region's nucleotide sequence