Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1584 and GFF1585 are separated by 172 nucleotides GFF1585 and GFF1586 are separated by 4 nucleotides
GFF1584 - Fumarate hydratase class I, alpha region (EC 4.2.1.2); L(+)-tartrate dehydratase alpha subunit (EC 4.2.1.32), at 182,786 to 183,658
GFF1584
GFF1585 - Outer membrane porin protein 32 precursor, at 183,831 to 185,048
GFF1585
GFF1586 - 2-dehydropantoate 2-reductase (EC 1.1.1.169), at 185,053 to 186,078
GFF1586
Position (kb)
183
184
185
186 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 182.876 kb on + strand, within GFF1584 at 183.246 kb on + strand, within GFF1584 at 183.247 kb on - strand, within GFF1584 at 183.301 kb on - strand, within GFF1584 at 183.301 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.442 kb on - strand, within GFF1584 at 183.612 kb on + strand at 183.612 kb on + strand at 183.663 kb on - strand at 183.663 kb on - strand at 184.294 kb on - strand, within GFF1585 at 184.395 kb on + strand, within GFF1585 at 184.396 kb on - strand, within GFF1585 at 184.396 kb on - strand, within GFF1585 at 184.396 kb on - strand, within GFF1585 at 184.425 kb on + strand, within GFF1585 at 184.426 kb on - strand, within GFF1585 at 184.426 kb on - strand, within GFF1585 at 184.440 kb on + strand, within GFF1585 at 184.441 kb on - strand, within GFF1585 at 184.441 kb on - strand, within GFF1585 at 184.441 kb on - strand, within GFF1585 at 184.596 kb on + strand, within GFF1585 at 184.596 kb on + strand, within GFF1585 at 184.597 kb on - strand, within GFF1585 at 184.648 kb on - strand, within GFF1585 at 184.727 kb on + strand, within GFF1585 at 184.728 kb on - strand, within GFF1585 at 184.806 kb on + strand, within GFF1585 at 184.807 kb on - strand, within GFF1585 at 184.807 kb on - strand, within GFF1585 at 184.821 kb on + strand, within GFF1585 at 184.884 kb on + strand, within GFF1585 at 184.884 kb on + strand, within GFF1585 at 184.885 kb on - strand, within GFF1585 at 184.885 kb on - strand, within GFF1585 at 184.885 kb on - strand, within GFF1585 at 184.917 kb on + strand, within GFF1585 at 184.917 kb on + strand, within GFF1585 at 184.918 kb on - strand, within GFF1585 at 185.020 kb on - strand at 185.212 kb on + strand, within GFF1586 at 185.404 kb on + strand, within GFF1586 at 185.404 kb on + strand, within GFF1586 at 185.549 kb on + strand, within GFF1586 at 185.550 kb on - strand, within GFF1586
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 182,876 + GFF1584 0.10 -0.5 183,246 + GFF1584 0.53 -1.1 183,247 - GFF1584 0.53 +0.7 183,301 - GFF1584 0.59 -1.0 183,301 - GFF1584 0.59 -1.3 183,442 - GFF1584 0.75 -2.4 183,442 - GFF1584 0.75 -0.1 183,442 - GFF1584 0.75 -0.0 183,612 + -0.5 183,612 + -0.8 183,663 - -0.4 183,663 - -0.6 184,294 - GFF1585 0.38 +0.2 184,395 + GFF1585 0.46 -3.1 184,396 - GFF1585 0.46 -0.5 184,396 - GFF1585 0.46 +0.4 184,396 - GFF1585 0.46 -0.9 184,425 + GFF1585 0.49 -0.1 184,426 - GFF1585 0.49 -0.6 184,426 - GFF1585 0.49 +0.0 184,440 + GFF1585 0.50 -0.2 184,441 - GFF1585 0.50 -1.2 184,441 - GFF1585 0.50 +0.1 184,441 - GFF1585 0.50 +0.6 184,596 + GFF1585 0.63 -0.2 184,596 + GFF1585 0.63 +0.0 184,597 - GFF1585 0.63 +0.1 184,648 - GFF1585 0.67 +0.3 184,727 + GFF1585 0.74 +0.3 184,728 - GFF1585 0.74 -0.6 184,806 + GFF1585 0.80 +0.2 184,807 - GFF1585 0.80 -1.3 184,807 - GFF1585 0.80 -0.4 184,821 + GFF1585 0.81 -0.1 184,884 + GFF1585 0.86 +0.2 184,884 + GFF1585 0.86 +0.4 184,885 - GFF1585 0.87 -0.1 184,885 - GFF1585 0.87 +0.0 184,885 - GFF1585 0.87 +0.2 184,917 + GFF1585 0.89 +0.3 184,917 + GFF1585 0.89 -0.3 184,918 - GFF1585 0.89 -0.3 185,020 - +0.5 185,212 + GFF1586 0.15 -0.6 185,404 + GFF1586 0.34 -1.1 185,404 + GFF1586 0.34 -0.5 185,549 + GFF1586 0.48 -0.3 185,550 - GFF1586 0.48 -1.7
Or see this region's nucleotide sequence