Strain Fitness in Variovorax sp. SCN45 around GFF1543

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1542 and GFF1543 are separated by 3 nucleotidesGFF1543 and GFF1544 are separated by 2 nucleotides GFF1542 - Anti-sigma B factor RsbT / Phosphoserine phosphatase RsbX (EC 3.1.3.3), at 139,176 to 140,171 GFF1542 GFF1543 - Putative SigmaB asociated two-component system sensor protein, at 140,175 to 141,053 GFF1543 GFF1544 - Two-component system sensor histidine kinase/response regulator hybrid, at 141,056 to 143,044 GFF1544 Position (kb) 140 141 142Strain fitness (log2 ratio) -2 -1 0 1at 139.835 kb on - strand, within GFF1542at 139.876 kb on - strand, within GFF1542at 139.876 kb on - strand, within GFF1542at 140.120 kb on + strandat 140.120 kb on + strandat 140.120 kb on + strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.121 kb on - strandat 140.296 kb on - strand, within GFF1543at 140.364 kb on + strand, within GFF1543at 140.364 kb on + strand, within GFF1543at 140.365 kb on - strand, within GFF1543at 140.558 kb on + strand, within GFF1543at 140.559 kb on - strand, within GFF1543at 140.682 kb on + strand, within GFF1543at 140.683 kb on - strand, within GFF1543at 140.828 kb on + strand, within GFF1543at 140.829 kb on - strand, within GFF1543at 141.140 kb on + strandat 141.141 kb on - strandat 141.398 kb on + strand, within GFF1544at 141.398 kb on + strand, within GFF1544at 141.399 kb on - strand, within GFF1544at 141.399 kb on - strand, within GFF1544at 141.512 kb on + strand, within GFF1544at 141.512 kb on + strand, within GFF1544at 141.512 kb on + strand, within GFF1544at 141.513 kb on - strand, within GFF1544at 141.513 kb on - strand, within GFF1544

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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139,835 - GFF1542 0.66 -1.1
139,876 - GFF1542 0.70 +0.3
139,876 - GFF1542 0.70 -0.6
140,120 + -2.0
140,120 + -0.1
140,120 + +0.1
140,121 - -0.1
140,121 - -0.3
140,121 - +0.5
140,121 - -1.8
140,121 - +0.7
140,121 - -0.5
140,121 - +0.3
140,121 - +0.4
140,121 - +0.1
140,296 - GFF1543 0.14 -0.9
140,364 + GFF1543 0.22 +0.3
140,364 + GFF1543 0.22 +0.4
140,365 - GFF1543 0.22 -0.1
140,558 + GFF1543 0.44 -0.4
140,559 - GFF1543 0.44 +1.1
140,682 + GFF1543 0.58 +0.1
140,683 - GFF1543 0.58 +0.9
140,828 + GFF1543 0.74 -0.3
140,829 - GFF1543 0.74 +0.6
141,140 + +0.2
141,141 - +0.1
141,398 + GFF1544 0.17 +0.4
141,398 + GFF1544 0.17 +0.3
141,399 - GFF1544 0.17 -1.3
141,399 - GFF1544 0.17 +0.0
141,512 + GFF1544 0.23 +0.3
141,512 + GFF1544 0.23 -0.1
141,512 + GFF1544 0.23 -0.2
141,513 - GFF1544 0.23 -1.0
141,513 - GFF1544 0.23 +0.0

Or see this region's nucleotide sequence