Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1481 and GFF1482 overlap by 4 nucleotides GFF1482 and GFF1483 overlap by 13 nucleotides GFF1483 and GFF1484 are separated by 101 nucleotides
GFF1481 - L-carnitine dehydratase/bile acid-inducible protein F, at 73,630 to 74,874
GFF1481
GFF1482 - Enoyl-CoA hydratase (EC 4.2.1.17), at 74,871 to 75,632
GFF1482
GFF1483 - Ribulose-5-phosphate 4-epimerase and related epimerases and aldolases, at 75,620 to 76,423
GFF1483
GFF1484 - Transcriptional regulator, LysR family, at 76,525 to 77,406
GFF1484
Position (kb) 74
75
76 Strain fitness (log2 ratio) -1
0
1 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 74.020 kb on + strand, within GFF1481 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.273 kb on + strand, within GFF1482 at 75.276 kb on + strand, within GFF1482 at 75.436 kb on - strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.472 kb on + strand, within GFF1482 at 75.512 kb on + strand, within GFF1482 at 75.543 kb on + strand, within GFF1482 at 75.740 kb on - strand, within GFF1483 at 75.976 kb on - strand, within GFF1483 at 76.405 kb on + strand at 76.485 kb on - strand at 76.505 kb on + strand at 76.506 kb on - strand at 76.513 kb on + strand at 76.513 kb on + strand at 76.514 kb on - strand at 76.514 kb on - strand at 76.516 kb on + strand at 76.532 kb on + strand at 76.533 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 73,984 + GFF1481 0.28 +0.1 73,984 + GFF1481 0.28 -0.1 73,984 + GFF1481 0.28 -0.1 73,985 - GFF1481 0.29 -1.0 73,985 - GFF1481 0.29 -1.3 73,985 - GFF1481 0.29 -0.2 74,020 + GFF1481 0.31 +0.2 75,273 + GFF1482 0.53 +0.7 75,273 + GFF1482 0.53 -1.3 75,273 + GFF1482 0.53 -0.3 75,276 + GFF1482 0.53 -1.1 75,436 - GFF1482 0.74 -1.3 75,472 + GFF1482 0.79 -1.0 75,472 + GFF1482 0.79 +1.7 75,472 + GFF1482 0.79 +0.0 75,512 + GFF1482 0.84 +0.8 75,543 + GFF1482 0.88 -0.6 75,740 - GFF1483 0.15 +1.7 75,976 - GFF1483 0.44 +0.1 76,405 + +0.3 76,485 - +1.1 76,505 + +0.0 76,506 - -1.5 76,513 + +0.6 76,513 + -1.3 76,514 - -0.9 76,514 - -0.1 76,516 + -0.3 76,532 + +0.5 76,533 - +0.5
Or see this region's nucleotide sequence