Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1479 and GFF1480 are separated by 27 nucleotides GFF1480 and GFF1481 are separated by 66 nucleotides
GFF1479 - Aldehyde dehydrogenase (EC 1.2.1.3), at 70,516 to 72,012
GFF1479
GFF1480 - Choline-sulfatase (EC 3.1.6.6), at 72,040 to 73,563
GFF1480
GFF1481 - L-carnitine dehydratase/bile acid-inducible protein F, at 73,630 to 74,874
GFF1481
Position (kb) 72
73
74 Strain fitness (log2 ratio) -2
-1
0
1 at 71.628 kb on - strand, within GFF1479 at 71.628 kb on - strand, within GFF1479 at 71.733 kb on + strand, within GFF1479 at 71.733 kb on + strand, within GFF1479 at 71.734 kb on - strand, within GFF1479 at 71.734 kb on - strand, within GFF1479 at 71.737 kb on + strand, within GFF1479 at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.017 kb on + strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.018 kb on - strand at 72.080 kb on - strand at 72.113 kb on + strand at 72.113 kb on + strand at 72.114 kb on - strand at 72.332 kb on + strand, within GFF1480 at 72.333 kb on - strand, within GFF1480 at 72.333 kb on - strand, within GFF1480 at 72.347 kb on + strand, within GFF1480 at 72.365 kb on + strand, within GFF1480 at 72.366 kb on - strand, within GFF1480 at 72.422 kb on + strand, within GFF1480 at 72.423 kb on - strand, within GFF1480 at 72.780 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.795 kb on - strand, within GFF1480 at 72.797 kb on + strand, within GFF1480 at 72.798 kb on - strand, within GFF1480 at 72.992 kb on + strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.080 kb on - strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.148 kb on + strand, within GFF1480 at 73.149 kb on - strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.202 kb on + strand, within GFF1480 at 73.203 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.287 kb on - strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.304 kb on + strand, within GFF1480 at 73.305 kb on - strand, within GFF1480 at 73.307 kb on + strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.308 kb on - strand, within GFF1480 at 73.427 kb on + strand at 73.428 kb on - strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.441 kb on + strand at 73.442 kb on - strand at 73.605 kb on - strand at 73.612 kb on - strand at 73.612 kb on - strand at 73.613 kb on + strand at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.834 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.837 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.984 kb on + strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 73.985 kb on - strand, within GFF1481 at 74.020 kb on + strand, within GFF1481
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 71,628 - GFF1479 0.74 +0.3 71,628 - GFF1479 0.74 +1.0 71,733 + GFF1479 0.81 -0.2 71,733 + GFF1479 0.81 -0.1 71,734 - GFF1479 0.81 +0.9 71,734 - GFF1479 0.81 -0.9 71,737 + GFF1479 0.82 +0.3 72,017 + -2.5 72,017 + +0.1 72,017 + +0.1 72,017 + -0.3 72,017 + +0.4 72,017 + +1.7 72,018 - -0.2 72,018 - +1.1 72,018 - -0.4 72,018 - +1.1 72,018 - -1.0 72,018 - -0.1 72,018 - -0.3 72,018 - +0.5 72,080 - -0.1 72,113 + -1.0 72,113 + -0.6 72,114 - -0.4 72,332 + GFF1480 0.19 -0.1 72,333 - GFF1480 0.19 -0.6 72,333 - GFF1480 0.19 -0.4 72,347 + GFF1480 0.20 -0.5 72,365 + GFF1480 0.21 +0.2 72,366 - GFF1480 0.21 -0.5 72,422 + GFF1480 0.25 +0.2 72,423 - GFF1480 0.25 -0.9 72,780 - GFF1480 0.49 -0.3 72,795 - GFF1480 0.50 -1.3 72,795 - GFF1480 0.50 -0.3 72,797 + GFF1480 0.50 +0.1 72,798 - GFF1480 0.50 -0.3 72,992 + GFF1480 0.62 -0.9 73,080 - GFF1480 0.68 -0.3 73,080 - GFF1480 0.68 +0.0 73,148 + GFF1480 0.73 +0.3 73,148 + GFF1480 0.73 -1.1 73,149 - GFF1480 0.73 +0.3 73,202 + GFF1480 0.76 -0.7 73,202 + GFF1480 0.76 -0.0 73,203 - GFF1480 0.76 -1.3 73,287 - GFF1480 0.82 -0.2 73,287 - GFF1480 0.82 -0.2 73,304 + GFF1480 0.83 -0.1 73,304 + GFF1480 0.83 -0.4 73,305 - GFF1480 0.83 -1.1 73,307 + GFF1480 0.83 -0.9 73,308 - GFF1480 0.83 -0.3 73,308 - GFF1480 0.83 +0.3 73,427 + -1.3 73,428 - -0.2 73,441 + +0.4 73,441 + +1.7 73,441 + -0.3 73,442 - -0.9 73,605 - +0.5 73,612 - -0.2 73,612 - +0.4 73,613 + +0.9 73,834 + GFF1481 0.16 -0.1 73,834 + GFF1481 0.16 -0.3 73,834 + GFF1481 0.16 +1.3 73,834 + GFF1481 0.16 +0.4 73,834 + GFF1481 0.16 -0.6 73,837 + GFF1481 0.17 +0.2 73,837 + GFF1481 0.17 -0.7 73,837 + GFF1481 0.17 +0.4 73,837 + GFF1481 0.17 -0.7 73,837 + GFF1481 0.17 -1.4 73,984 + GFF1481 0.28 +0.1 73,984 + GFF1481 0.28 -0.1 73,984 + GFF1481 0.28 -0.1 73,985 - GFF1481 0.29 -1.0 73,985 - GFF1481 0.29 -1.3 73,985 - GFF1481 0.29 -0.2 74,020 + GFF1481 0.31 +0.2
Or see this region's nucleotide sequence