Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1336 and GFF1337 are separated by 218 nucleotides GFF1337 and GFF1338 are separated by 80 nucleotides
GFF1336 - ABC transporter, substrate-binding protein RSc1750, at 677,188 to 678,417
GFF1336
GFF1337 - ABC transporter, substrate-binding protein RSc1750, at 678,636 to 679,868
GFF1337
GFF1338 - ABC transporter, permease protein 1 RSc1751, at 679,949 to 680,902
GFF1338
Position (kb) 678
679
680 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 677.707 kb on + strand, within GFF1336 at 677.708 kb on - strand, within GFF1336 at 677.708 kb on - strand, within GFF1336 at 677.725 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.752 kb on + strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.753 kb on - strand, within GFF1336 at 677.845 kb on + strand, within GFF1336 at 677.846 kb on - strand, within GFF1336 at 677.846 kb on - strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.965 kb on + strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.966 kb on - strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.968 kb on + strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 677.969 kb on - strand, within GFF1336 at 678.026 kb on - strand, within GFF1336 at 678.026 kb on - strand, within GFF1336 at 678.037 kb on + strand, within GFF1336 at 678.037 kb on + strand, within GFF1336 at 678.038 kb on - strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.046 kb on + strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.047 kb on - strand, within GFF1336 at 678.086 kb on - strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.127 kb on + strand, within GFF1336 at 678.128 kb on - strand, within GFF1336 at 678.128 kb on - strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.280 kb on + strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.281 kb on - strand, within GFF1336 at 678.313 kb on + strand at 678.313 kb on + strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.314 kb on - strand at 678.361 kb on + strand at 678.361 kb on + strand at 678.361 kb on + strand at 678.362 kb on - strand at 678.364 kb on + strand at 678.364 kb on + strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.365 kb on - strand at 678.373 kb on + strand at 678.374 kb on - strand at 678.374 kb on - strand at 678.542 kb on + strand at 678.542 kb on + strand at 678.542 kb on + strand at 678.581 kb on - strand at 678.581 kb on - strand at 678.588 kb on + strand at 678.589 kb on - strand at 678.630 kb on + strand at 678.630 kb on + strand at 678.630 kb on + strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.631 kb on - strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.632 kb on + strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.633 kb on - strand at 678.922 kb on - strand, within GFF1337 at 678.922 kb on - strand, within GFF1337 at 679.014 kb on + strand, within GFF1337 at 679.015 kb on - strand, within GFF1337 at 679.065 kb on + strand, within GFF1337 at 679.066 kb on - strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.122 kb on + strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.123 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.156 kb on - strand, within GFF1337 at 679.173 kb on + strand, within GFF1337 at 679.174 kb on - strand, within GFF1337 at 679.200 kb on + strand, within GFF1337 at 679.200 kb on + strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.201 kb on - strand, within GFF1337 at 679.293 kb on + strand, within GFF1337 at 679.294 kb on - strand, within GFF1337 at 679.407 kb on + strand, within GFF1337 at 679.407 kb on + strand, within GFF1337 at 679.408 kb on - strand, within GFF1337 at 679.408 kb on - strand, within GFF1337 at 679.413 kb on + strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.414 kb on - strand, within GFF1337 at 679.416 kb on + strand, within GFF1337 at 679.416 kb on + strand, within GFF1337 at 679.473 kb on + strand, within GFF1337 at 679.473 kb on + strand, within GFF1337 at 679.473 kb on + strand, within GFF1337 at 679.473 kb on + strand, within GFF1337 at 679.473 kb on + strand, within GFF1337 at 679.474 kb on - strand, within GFF1337 at 679.494 kb on + strand, within GFF1337 at 679.495 kb on - strand, within GFF1337 at 679.495 kb on - strand, within GFF1337 at 679.495 kb on - strand, within GFF1337 at 679.522 kb on - strand, within GFF1337 at 679.561 kb on - strand, within GFF1337 at 679.561 kb on - strand, within GFF1337 at 679.575 kb on + strand, within GFF1337 at 679.575 kb on + strand, within GFF1337 at 679.576 kb on - strand, within GFF1337 at 679.576 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.729 kb on - strand, within GFF1337 at 679.761 kb on + strand at 679.761 kb on + strand at 679.768 kb on - strand at 679.809 kb on + strand at 679.809 kb on + strand at 679.810 kb on - strand at 679.812 kb on + strand at 679.812 kb on + strand at 679.812 kb on + strand at 679.813 kb on - strand at 679.813 kb on - strand at 679.813 kb on - strand at 679.821 kb on + strand at 679.821 kb on + strand at 679.821 kb on + strand at 679.994 kb on + strand at 679.994 kb on + strand at 679.994 kb on + strand at 679.994 kb on + strand at 679.994 kb on + strand at 679.995 kb on - strand at 679.995 kb on - strand at 679.995 kb on - strand at 679.995 kb on - strand at 679.995 kb on - strand at 680.081 kb on + strand, within GFF1338 at 680.082 kb on - strand, within GFF1338 at 680.097 kb on - strand, within GFF1338 at 680.193 kb on - strand, within GFF1338 at 680.258 kb on + strand, within GFF1338 at 680.351 kb on + strand, within GFF1338 at 680.585 kb on + strand, within GFF1338 at 680.585 kb on + strand, within GFF1338 at 680.585 kb on + strand, within GFF1338 at 680.585 kb on + strand, within GFF1338 at 680.586 kb on - strand, within GFF1338 at 680.586 kb on - strand, within GFF1338 at 680.586 kb on - strand, within GFF1338 at 680.790 kb on - strand, within GFF1338 at 680.837 kb on + strand at 680.837 kb on + strand at 680.838 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 677,707 + GFF1336 0.42 -0.3 677,708 - GFF1336 0.42 -0.3 677,708 - GFF1336 0.42 +1.0 677,725 + GFF1336 0.44 -0.5 677,752 + GFF1336 0.46 -1.3 677,752 + GFF1336 0.46 -0.1 677,752 + GFF1336 0.46 -0.7 677,753 - GFF1336 0.46 -0.5 677,753 - GFF1336 0.46 -0.6 677,753 - GFF1336 0.46 -0.8 677,845 + GFF1336 0.53 +0.7 677,846 - GFF1336 0.53 +0.1 677,846 - GFF1336 0.53 +0.6 677,965 + GFF1336 0.63 +0.3 677,965 + GFF1336 0.63 +0.5 677,965 + GFF1336 0.63 -1.1 677,966 - GFF1336 0.63 +0.1 677,966 - GFF1336 0.63 -0.3 677,966 - GFF1336 0.63 -0.6 677,966 - GFF1336 0.63 -0.1 677,966 - GFF1336 0.63 -0.0 677,968 + GFF1336 0.63 +0.9 677,968 + GFF1336 0.63 +1.1 677,968 + GFF1336 0.63 -1.0 677,968 + GFF1336 0.63 -0.7 677,969 - GFF1336 0.63 -0.3 677,969 - GFF1336 0.63 -2.3 677,969 - GFF1336 0.63 +0.5 678,026 - GFF1336 0.68 +1.9 678,026 - GFF1336 0.68 +0.4 678,037 + GFF1336 0.69 -1.0 678,037 + GFF1336 0.69 -0.4 678,038 - GFF1336 0.69 +0.1 678,046 + GFF1336 0.70 +1.8 678,046 + GFF1336 0.70 -0.0 678,046 + GFF1336 0.70 +0.2 678,046 + GFF1336 0.70 -0.3 678,047 - GFF1336 0.70 +1.0 678,047 - GFF1336 0.70 -1.3 678,047 - GFF1336 0.70 -0.0 678,086 - GFF1336 0.73 -1.1 678,127 + GFF1336 0.76 +0.4 678,127 + GFF1336 0.76 -0.2 678,127 + GFF1336 0.76 -0.4 678,128 - GFF1336 0.76 +0.8 678,128 - GFF1336 0.76 -0.6 678,280 + GFF1336 0.89 -0.0 678,280 + GFF1336 0.89 -0.0 678,280 + GFF1336 0.89 -1.3 678,280 + GFF1336 0.89 -0.0 678,280 + GFF1336 0.89 +1.0 678,280 + GFF1336 0.89 +1.0 678,280 + GFF1336 0.89 +0.3 678,280 + GFF1336 0.89 +0.3 678,281 - GFF1336 0.89 -0.0 678,281 - GFF1336 0.89 -1.0 678,281 - GFF1336 0.89 +0.1 678,281 - GFF1336 0.89 +0.1 678,281 - GFF1336 0.89 +0.1 678,281 - GFF1336 0.89 -0.7 678,281 - GFF1336 0.89 +0.0 678,281 - GFF1336 0.89 +0.8 678,281 - GFF1336 0.89 +3.3 678,313 + +0.5 678,313 + +2.1 678,314 - +0.2 678,314 - +0.2 678,314 - -0.7 678,314 - -0.3 678,361 + -0.1 678,361 + +0.2 678,361 + -0.8 678,362 - +1.9 678,364 + -0.7 678,364 + -0.3 678,365 - +1.3 678,365 - +0.0 678,365 - -0.6 678,365 - -0.3 678,373 + +0.8 678,374 - +0.1 678,374 - -0.7 678,542 + -0.5 678,542 + -0.2 678,542 + +0.3 678,581 - -0.6 678,581 - -0.3 678,588 + -0.6 678,589 - +0.7 678,630 + -0.5 678,630 + -1.6 678,630 + -0.2 678,631 - -0.8 678,631 - -0.6 678,631 - -1.1 678,631 - -0.2 678,631 - -0.3 678,632 + +1.5 678,632 + +0.3 678,632 + -0.5 678,632 + -0.2 678,633 - +0.1 678,633 - -0.8 678,633 - -0.6 678,633 - +1.1 678,633 - -1.0 678,633 - +2.3 678,633 - +1.5 678,633 - -0.6 678,922 - GFF1337 0.23 -0.8 678,922 - GFF1337 0.23 +1.3 679,014 + GFF1337 0.31 -1.7 679,015 - GFF1337 0.31 +0.3 679,065 + GFF1337 0.35 +0.3 679,066 - GFF1337 0.35 -0.3 679,122 + GFF1337 0.39 -0.6 679,122 + GFF1337 0.39 -1.0 679,122 + GFF1337 0.39 +1.3 679,122 + GFF1337 0.39 -2.0 679,122 + GFF1337 0.39 -0.4 679,122 + GFF1337 0.39 -0.4 679,122 + GFF1337 0.39 -0.9 679,123 - GFF1337 0.39 +0.5 679,123 - GFF1337 0.39 -0.9 679,123 - GFF1337 0.39 -0.1 679,123 - GFF1337 0.39 -0.2 679,123 - GFF1337 0.39 +0.0 679,123 - GFF1337 0.39 -0.9 679,123 - GFF1337 0.39 +0.4 679,156 - GFF1337 0.42 -0.3 679,156 - GFF1337 0.42 +1.0 679,156 - GFF1337 0.42 -0.7 679,156 - GFF1337 0.42 +0.5 679,173 + GFF1337 0.44 +0.0 679,174 - GFF1337 0.44 -2.4 679,200 + GFF1337 0.46 +0.7 679,200 + GFF1337 0.46 -0.1 679,201 - GFF1337 0.46 -0.6 679,201 - GFF1337 0.46 -1.2 679,201 - GFF1337 0.46 +0.1 679,293 + GFF1337 0.53 -1.4 679,294 - GFF1337 0.53 -0.5 679,407 + GFF1337 0.63 +0.4 679,407 + GFF1337 0.63 -0.5 679,408 - GFF1337 0.63 -0.6 679,408 - GFF1337 0.63 -0.3 679,413 + GFF1337 0.63 -0.3 679,414 - GFF1337 0.63 -1.9 679,414 - GFF1337 0.63 -1.5 679,414 - GFF1337 0.63 -0.6 679,414 - GFF1337 0.63 -0.1 679,414 - GFF1337 0.63 -0.5 679,416 + GFF1337 0.63 -2.1 679,416 + GFF1337 0.63 -1.0 679,473 + GFF1337 0.68 -3.3 679,473 + GFF1337 0.68 -1.2 679,473 + GFF1337 0.68 -1.3 679,473 + GFF1337 0.68 -0.4 679,473 + GFF1337 0.68 -0.3 679,474 - GFF1337 0.68 +0.2 679,494 + GFF1337 0.70 +1.7 679,495 - GFF1337 0.70 -0.2 679,495 - GFF1337 0.70 -0.9 679,495 - GFF1337 0.70 -0.8 679,522 - GFF1337 0.72 -1.6 679,561 - GFF1337 0.75 -0.6 679,561 - GFF1337 0.75 -0.1 679,575 + GFF1337 0.76 -0.3 679,575 + GFF1337 0.76 -0.0 679,576 - GFF1337 0.76 +0.3 679,576 - GFF1337 0.76 +0.1 679,729 - GFF1337 0.89 -0.5 679,729 - GFF1337 0.89 +0.4 679,729 - GFF1337 0.89 +0.4 679,729 - GFF1337 0.89 +0.4 679,729 - GFF1337 0.89 +1.0 679,729 - GFF1337 0.89 +1.0 679,729 - GFF1337 0.89 +1.1 679,761 + -0.4 679,761 + -0.7 679,768 - +0.1 679,809 + +0.4 679,809 + +0.1 679,810 - +1.0 679,812 + +0.1 679,812 + -0.3 679,812 + -1.3 679,813 - -1.7 679,813 - -0.6 679,813 - +1.5 679,821 + -0.6 679,821 + -1.0 679,821 + +0.2 679,994 + -1.1 679,994 + -0.0 679,994 + -1.9 679,994 + -1.5 679,994 + -0.3 679,995 - +0.9 679,995 - -0.0 679,995 - +0.5 679,995 - -0.7 679,995 - -0.6 680,081 + GFF1338 0.14 -0.5 680,082 - GFF1338 0.14 -0.7 680,097 - GFF1338 0.16 +0.3 680,193 - GFF1338 0.26 +1.6 680,258 + GFF1338 0.32 -0.5 680,351 + GFF1338 0.42 -0.7 680,585 + GFF1338 0.67 -0.1 680,585 + GFF1338 0.67 -2.1 680,585 + GFF1338 0.67 -0.2 680,585 + GFF1338 0.67 -1.0 680,586 - GFF1338 0.67 +2.1 680,586 - GFF1338 0.67 -0.5 680,586 - GFF1338 0.67 -0.3 680,790 - GFF1338 0.88 -0.7 680,837 + -2.9 680,837 + -0.3 680,838 - -0.5
Or see this region's nucleotide sequence