Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1303 and GFF1304 are separated by 133 nucleotides GFF1304 and GFF1305 overlap by 4 nucleotides
GFF1303 - Glutamate/aspartate ABC transporter, substrate-binding protein GltI (TC 3.A.1.3.4), at 633,611 to 634,648
GFF1303
GFF1304 - Glutamate/aspartate ABC transporter, permease protein GltJ (TC 3.A.1.3.4), at 634,782 to 635,789
GFF1304
GFF1305 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 635,786 to 636,841
GFF1305
Position (kb)
634
635
636 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.989 kb on + strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.990 kb on - strand, within GFF1303 at 633.992 kb on + strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 633.993 kb on - strand, within GFF1303 at 634.109 kb on + strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.110 kb on - strand, within GFF1303 at 634.265 kb on + strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.266 kb on - strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.535 kb on + strand, within GFF1303 at 634.536 kb on - strand, within GFF1303 at 634.536 kb on - strand, within GFF1303 at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.628 kb on + strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 634.629 kb on - strand at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.145 kb on + strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.146 kb on - strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.321 kb on + strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.322 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.349 kb on - strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.388 kb on + strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.389 kb on - strand, within GFF1304 at 635.416 kb on + strand, within GFF1304 at 635.419 kb on - strand, within GFF1304 at 635.419 kb on - strand, within GFF1304 at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.712 kb on + strand at 635.713 kb on - strand at 635.713 kb on - strand at 635.713 kb on - strand at 635.754 kb on + strand at 635.754 kb on + strand at 635.754 kb on + strand at 636.335 kb on + strand, within GFF1305 at 636.335 kb on + strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.336 kb on - strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.476 kb on + strand, within GFF1305 at 636.477 kb on - strand, within GFF1305 at 636.737 kb on + strand at 636.737 kb on + strand at 636.737 kb on + strand at 636.738 kb on - strand at 636.738 kb on - strand at 636.749 kb on + strand at 636.750 kb on - strand at 636.770 kb on + strand at 636.770 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 633,989 + GFF1303 0.36 -0.3 633,989 + GFF1303 0.36 +0.8 633,989 + GFF1303 0.36 -0.7 633,989 + GFF1303 0.36 +0.7 633,989 + GFF1303 0.36 +0.4 633,989 + GFF1303 0.36 +0.2 633,989 + GFF1303 0.36 -0.6 633,989 + GFF1303 0.36 +0.7 633,990 - GFF1303 0.37 -0.8 633,990 - GFF1303 0.37 -2.2 633,990 - GFF1303 0.37 -1.0 633,990 - GFF1303 0.37 -0.1 633,990 - GFF1303 0.37 -1.0 633,990 - GFF1303 0.37 -0.5 633,992 + GFF1303 0.37 -0.0 633,993 - GFF1303 0.37 -0.9 633,993 - GFF1303 0.37 -1.0 633,993 - GFF1303 0.37 -1.6 633,993 - GFF1303 0.37 +0.1 634,109 + GFF1303 0.48 -0.9 634,110 - GFF1303 0.48 -0.3 634,110 - GFF1303 0.48 +1.0 634,110 - GFF1303 0.48 -1.3 634,265 + GFF1303 0.63 +0.2 634,266 - GFF1303 0.63 +0.2 634,266 - GFF1303 0.63 -0.1 634,266 - GFF1303 0.63 -0.1 634,266 - GFF1303 0.63 -0.1 634,266 - GFF1303 0.63 +1.1 634,535 + GFF1303 0.89 -0.7 634,535 + GFF1303 0.89 -0.2 634,535 + GFF1303 0.89 -0.7 634,535 + GFF1303 0.89 -0.8 634,536 - GFF1303 0.89 -0.2 634,536 - GFF1303 0.89 -0.0 634,628 + +0.3 634,628 + -1.3 634,628 + +1.3 634,628 + -0.2 634,628 + -0.3 634,628 + -0.9 634,628 + -0.4 634,628 + -0.4 634,628 + -0.0 634,628 + +1.3 634,628 + -0.5 634,629 - +0.2 634,629 - -0.5 634,629 - +0.0 634,629 - -0.3 634,629 - +0.6 634,629 - -0.5 634,629 - -0.8 634,629 - -0.1 634,629 - -0.5 634,629 - -0.0 634,629 - -0.5 634,629 - -1.3 634,629 - +1.3 634,629 - -0.5 634,629 - -1.0 634,629 - +0.3 634,629 - -0.2 634,629 - -0.1 635,145 + GFF1304 0.36 -2.1 635,145 + GFF1304 0.36 -1.5 635,145 + GFF1304 0.36 +0.3 635,146 - GFF1304 0.36 -0.4 635,146 - GFF1304 0.36 -0.8 635,146 - GFF1304 0.36 -0.3 635,146 - GFF1304 0.36 +0.3 635,146 - GFF1304 0.36 +0.1 635,146 - GFF1304 0.36 -0.5 635,321 + GFF1304 0.53 -0.6 635,321 + GFF1304 0.53 -0.4 635,322 - GFF1304 0.54 -0.5 635,322 - GFF1304 0.54 -0.6 635,349 - GFF1304 0.56 +0.3 635,349 - GFF1304 0.56 -2.0 635,388 + GFF1304 0.60 +0.7 635,388 + GFF1304 0.60 +0.1 635,388 + GFF1304 0.60 +0.5 635,388 + GFF1304 0.60 +0.8 635,389 - GFF1304 0.60 -0.7 635,389 - GFF1304 0.60 +1.3 635,389 - GFF1304 0.60 -0.5 635,389 - GFF1304 0.60 -0.3 635,389 - GFF1304 0.60 -0.8 635,416 + GFF1304 0.63 -0.7 635,419 - GFF1304 0.63 -0.5 635,419 - GFF1304 0.63 -0.9 635,712 + -0.3 635,712 + -0.3 635,712 + -0.5 635,712 + +0.4 635,712 + -0.1 635,713 - +0.1 635,713 - +0.2 635,713 - -0.5 635,754 + -1.0 635,754 + +0.6 635,754 + -0.4 636,335 + GFF1305 0.52 +0.1 636,335 + GFF1305 0.52 +0.5 636,336 - GFF1305 0.52 +0.1 636,336 - GFF1305 0.52 -0.1 636,336 - GFF1305 0.52 -0.7 636,476 + GFF1305 0.65 -1.3 636,476 + GFF1305 0.65 +1.1 636,476 + GFF1305 0.65 -3.1 636,477 - GFF1305 0.65 -1.3 636,737 + +0.5 636,737 + -1.6 636,737 + -0.4 636,738 - -1.1 636,738 - +0.1 636,749 + -1.5 636,750 - -1.4 636,770 + -0.8 636,770 + -0.2
Or see this region's nucleotide sequence