Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1249 and GFF1250 are separated by 31 nucleotides GFF1250 and GFF1251 overlap by 1 nucleotides GFF1251 and GFF1252 are separated by 13 nucleotides GFF1252 and GFF1253 are separated by 10 nucleotides
GFF1249 - hypothetical protein, at 568,451 to 568,588
GFF1249
GFF1250 - Alkanesulfonate ABC transporter substrate-binding protein SsuA, at 568,620 to 569,576
GFF1250
GFF1251 - FMNH2-dependent alkanesulfonate monooxygenase (EC 1.14.14.5), at 569,576 to 570,745
GFF1251
GFF1252 - Alkanesulfonate ABC transporter permease protein SsuC, at 570,759 to 571,604
GFF1252
GFF1253 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 571,615 to 572,589
GFF1253
Position (kb)
569
570
571 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 568.746 kb on + strand, within GFF1250 at 568.746 kb on + strand, within GFF1250 at 568.747 kb on - strand, within GFF1250 at 568.935 kb on + strand, within GFF1250 at 568.936 kb on - strand, within GFF1250 at 568.936 kb on - strand, within GFF1250 at 569.051 kb on + strand, within GFF1250 at 569.061 kb on + strand, within GFF1250 at 569.061 kb on + strand, within GFF1250 at 569.061 kb on + strand, within GFF1250 at 569.061 kb on + strand, within GFF1250 at 569.121 kb on + strand, within GFF1250 at 569.269 kb on - strand, within GFF1250 at 569.269 kb on - strand, within GFF1250 at 569.271 kb on + strand, within GFF1250 at 569.272 kb on - strand, within GFF1250 at 569.272 kb on - strand, within GFF1250 at 569.272 kb on - strand, within GFF1250 at 569.272 kb on - strand, within GFF1250 at 569.272 kb on - strand, within GFF1250 at 569.289 kb on + strand, within GFF1250 at 569.289 kb on + strand, within GFF1250 at 569.290 kb on - strand, within GFF1250 at 569.618 kb on + strand at 569.660 kb on + strand at 569.661 kb on - strand at 569.661 kb on - strand at 569.661 kb on - strand at 569.700 kb on - strand, within GFF1251 at 569.934 kb on - strand, within GFF1251 at 569.934 kb on - strand, within GFF1251 at 569.955 kb on - strand, within GFF1251 at 570.029 kb on + strand, within GFF1251 at 570.071 kb on + strand, within GFF1251 at 570.093 kb on - strand, within GFF1251 at 570.161 kb on + strand, within GFF1251 at 570.162 kb on - strand, within GFF1251 at 570.474 kb on + strand, within GFF1251 at 570.474 kb on + strand, within GFF1251 at 570.608 kb on + strand, within GFF1251 at 570.772 kb on + strand at 570.772 kb on + strand at 570.773 kb on - strand at 570.950 kb on + strand, within GFF1252 at 571.236 kb on + strand, within GFF1252 at 571.236 kb on + strand, within GFF1252 at 571.237 kb on - strand, within GFF1252 at 571.509 kb on + strand, within GFF1252 at 571.510 kb on - strand, within GFF1252
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 568,746 + GFF1250 0.13 -0.3 568,746 + GFF1250 0.13 -1.2 568,747 - GFF1250 0.13 +0.6 568,935 + GFF1250 0.33 -0.7 568,936 - GFF1250 0.33 -0.1 568,936 - GFF1250 0.33 -1.7 569,051 + GFF1250 0.45 -0.2 569,061 + GFF1250 0.46 +0.5 569,061 + GFF1250 0.46 +0.4 569,061 + GFF1250 0.46 -1.9 569,061 + GFF1250 0.46 -0.9 569,121 + GFF1250 0.52 +1.3 569,269 - GFF1250 0.68 -1.2 569,269 - GFF1250 0.68 +0.2 569,271 + GFF1250 0.68 +2.3 569,272 - GFF1250 0.68 -0.8 569,272 - GFF1250 0.68 +0.3 569,272 - GFF1250 0.68 +0.1 569,272 - GFF1250 0.68 -0.2 569,272 - GFF1250 0.68 +0.8 569,289 + GFF1250 0.70 -1.4 569,289 + GFF1250 0.70 +0.4 569,290 - GFF1250 0.70 +0.5 569,618 + -0.7 569,660 + -0.3 569,661 - +0.2 569,661 - +0.1 569,661 - -0.9 569,700 - GFF1251 0.11 -0.7 569,934 - GFF1251 0.31 +0.7 569,934 - GFF1251 0.31 +0.2 569,955 - GFF1251 0.32 -1.2 570,029 + GFF1251 0.39 +0.9 570,071 + GFF1251 0.42 +0.4 570,093 - GFF1251 0.44 -0.0 570,161 + GFF1251 0.50 +0.9 570,162 - GFF1251 0.50 +0.7 570,474 + GFF1251 0.77 +0.5 570,474 + GFF1251 0.77 +1.0 570,608 + GFF1251 0.88 +0.1 570,772 + -0.5 570,772 + +0.6 570,773 - -0.0 570,950 + GFF1252 0.23 -0.3 571,236 + GFF1252 0.56 -0.8 571,236 + GFF1252 0.56 -0.3 571,237 - GFF1252 0.57 -0.5 571,509 + GFF1252 0.89 +1.0 571,510 - GFF1252 0.89 +0.2
Or see this region's nucleotide sequence