Strain Fitness in Variovorax sp. SCN45 around GFF1251

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1249 and GFF1250 are separated by 31 nucleotidesGFF1250 and GFF1251 overlap by 1 nucleotidesGFF1251 and GFF1252 are separated by 13 nucleotidesGFF1252 and GFF1253 are separated by 10 nucleotides GFF1249 - hypothetical protein, at 568,451 to 568,588 GFF1249 GFF1250 - Alkanesulfonate ABC transporter substrate-binding protein SsuA, at 568,620 to 569,576 GFF1250 GFF1251 - FMNH2-dependent alkanesulfonate monooxygenase (EC 1.14.14.5), at 569,576 to 570,745 GFF1251 GFF1252 - Alkanesulfonate ABC transporter permease protein SsuC, at 570,759 to 571,604 GFF1252 GFF1253 - ABC transporter, substrate-binding protein (cluster 10, nitrate/sulfonate/bicarbonate), at 571,615 to 572,589 GFF1253 Position (kb) 569 570 571Strain fitness (log2 ratio) -2 -1 0 1 2at 568.746 kb on + strand, within GFF1250at 568.746 kb on + strand, within GFF1250at 568.747 kb on - strand, within GFF1250at 568.935 kb on + strand, within GFF1250at 568.936 kb on - strand, within GFF1250at 568.936 kb on - strand, within GFF1250at 569.051 kb on + strand, within GFF1250at 569.061 kb on + strand, within GFF1250at 569.061 kb on + strand, within GFF1250at 569.061 kb on + strand, within GFF1250at 569.061 kb on + strand, within GFF1250at 569.121 kb on + strand, within GFF1250at 569.269 kb on - strand, within GFF1250at 569.269 kb on - strand, within GFF1250at 569.271 kb on + strand, within GFF1250at 569.272 kb on - strand, within GFF1250at 569.272 kb on - strand, within GFF1250at 569.272 kb on - strand, within GFF1250at 569.272 kb on - strand, within GFF1250at 569.272 kb on - strand, within GFF1250at 569.289 kb on + strand, within GFF1250at 569.289 kb on + strand, within GFF1250at 569.290 kb on - strand, within GFF1250at 569.618 kb on + strandat 569.660 kb on + strandat 569.661 kb on - strandat 569.661 kb on - strandat 569.661 kb on - strandat 569.700 kb on - strand, within GFF1251at 569.934 kb on - strand, within GFF1251at 569.934 kb on - strand, within GFF1251at 569.955 kb on - strand, within GFF1251at 570.029 kb on + strand, within GFF1251at 570.071 kb on + strand, within GFF1251at 570.093 kb on - strand, within GFF1251at 570.161 kb on + strand, within GFF1251at 570.162 kb on - strand, within GFF1251at 570.474 kb on + strand, within GFF1251at 570.474 kb on + strand, within GFF1251at 570.608 kb on + strand, within GFF1251at 570.772 kb on + strandat 570.772 kb on + strandat 570.773 kb on - strandat 570.950 kb on + strand, within GFF1252at 571.236 kb on + strand, within GFF1252at 571.236 kb on + strand, within GFF1252at 571.237 kb on - strand, within GFF1252at 571.509 kb on + strand, within GFF1252at 571.510 kb on - strand, within GFF1252

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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568,746 + GFF1250 0.13 -0.3
568,746 + GFF1250 0.13 -1.2
568,747 - GFF1250 0.13 +0.6
568,935 + GFF1250 0.33 -0.7
568,936 - GFF1250 0.33 -0.1
568,936 - GFF1250 0.33 -1.7
569,051 + GFF1250 0.45 -0.2
569,061 + GFF1250 0.46 +0.5
569,061 + GFF1250 0.46 +0.4
569,061 + GFF1250 0.46 -1.9
569,061 + GFF1250 0.46 -0.9
569,121 + GFF1250 0.52 +1.3
569,269 - GFF1250 0.68 -1.2
569,269 - GFF1250 0.68 +0.2
569,271 + GFF1250 0.68 +2.3
569,272 - GFF1250 0.68 -0.8
569,272 - GFF1250 0.68 +0.3
569,272 - GFF1250 0.68 +0.1
569,272 - GFF1250 0.68 -0.2
569,272 - GFF1250 0.68 +0.8
569,289 + GFF1250 0.70 -1.4
569,289 + GFF1250 0.70 +0.4
569,290 - GFF1250 0.70 +0.5
569,618 + -0.7
569,660 + -0.3
569,661 - +0.2
569,661 - +0.1
569,661 - -0.9
569,700 - GFF1251 0.11 -0.7
569,934 - GFF1251 0.31 +0.7
569,934 - GFF1251 0.31 +0.2
569,955 - GFF1251 0.32 -1.2
570,029 + GFF1251 0.39 +0.9
570,071 + GFF1251 0.42 +0.4
570,093 - GFF1251 0.44 -0.0
570,161 + GFF1251 0.50 +0.9
570,162 - GFF1251 0.50 +0.7
570,474 + GFF1251 0.77 +0.5
570,474 + GFF1251 0.77 +1.0
570,608 + GFF1251 0.88 +0.1
570,772 + -0.5
570,772 + +0.6
570,773 - -0.0
570,950 + GFF1252 0.23 -0.3
571,236 + GFF1252 0.56 -0.8
571,236 + GFF1252 0.56 -0.3
571,237 - GFF1252 0.57 -0.5
571,509 + GFF1252 0.89 +1.0
571,510 - GFF1252 0.89 +0.2

Or see this region's nucleotide sequence