Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF121 and GFF122 overlap by 4 nucleotides GFF122 and GFF123 overlap by 8 nucleotides GFF123 and GFF124 are separated by 16 nucleotides
GFF121 - Uncharacterized MFS-type transporter, at 149,556 to 150,818
GFF121
GFF122 - Dihydroorotate dehydrogenase electron transfer subunit (EC 1.3.3.1), at 150,815 to 151,690
GFF122
GFF123 - Dihydroorotate dehydrogenase (NAD(+)), catalytic subunit (EC 1.3.1.14), at 151,683 to 152,633
GFF123
GFF124 - Beta-ureidopropionase (EC 3.5.1.6), at 152,650 to 153,930
GFF124
Position (kb)
151
152
153 Strain fitness (log2 ratio)
-1
0
1
2 at 150.753 kb on + strand at 150.753 kb on + strand at 150.753 kb on + strand at 150.754 kb on - strand at 150.754 kb on - strand at 150.995 kb on + strand, within GFF122 at 151.058 kb on + strand, within GFF122 at 151.059 kb on - strand, within GFF122 at 151.376 kb on + strand, within GFF122 at 151.460 kb on + strand, within GFF122 at 151.764 kb on + strand at 151.915 kb on - strand, within GFF123 at 151.917 kb on + strand, within GFF123 at 151.941 kb on + strand, within GFF123 at 152.115 kb on + strand, within GFF123 at 152.115 kb on + strand, within GFF123 at 152.116 kb on - strand, within GFF123 at 152.368 kb on - strand, within GFF123 at 152.481 kb on - strand, within GFF123 at 152.527 kb on - strand, within GFF123 at 152.759 kb on - strand at 152.789 kb on - strand, within GFF124 at 152.907 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.067 kb on + strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.068 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.233 kb on - strand, within GFF124 at 153.590 kb on - strand, within GFF124
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 150,753 + -0.3 150,753 + +0.6 150,753 + +1.3 150,754 - -1.3 150,754 - +0.9 150,995 + GFF122 0.21 -0.7 151,058 + GFF122 0.28 +1.0 151,059 - GFF122 0.28 -0.7 151,376 + GFF122 0.64 -0.1 151,460 + GFF122 0.74 +1.8 151,764 + +0.3 151,915 - GFF123 0.24 +0.1 151,917 + GFF123 0.25 +0.2 151,941 + GFF123 0.27 +0.2 152,115 + GFF123 0.45 +0.8 152,115 + GFF123 0.45 -0.3 152,116 - GFF123 0.46 -0.3 152,368 - GFF123 0.72 +0.8 152,481 - GFF123 0.84 +0.3 152,527 - GFF123 0.89 -0.1 152,759 - -0.2 152,789 - GFF124 0.11 +0.3 152,907 + GFF124 0.20 -0.7 153,067 + GFF124 0.33 -0.2 153,067 + GFF124 0.33 +1.5 153,067 + GFF124 0.33 +0.5 153,068 - GFF124 0.33 -0.5 153,068 - GFF124 0.33 +0.1 153,068 - GFF124 0.33 +0.3 153,068 - GFF124 0.33 -1.5 153,233 - GFF124 0.46 -0.0 153,233 - GFF124 0.46 -0.0 153,590 - GFF124 0.73 -0.4
Or see this region's nucleotide sequence