Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1171 and GFF1172 are separated by 13 nucleotides GFF1172 and GFF1173 overlap by 4 nucleotides GFF1173 and GFF1174 are separated by 141 nucleotides GFF1174 and GFF1175 are separated by 63 nucleotides
GFF1171 - Transcriptional regulator, AraC family, at 487,924 to 488,823
GFF1171
GFF1172 - hypothetical protein, at 488,837 to 489,241
GFF1172
GFF1173 - P-hydroxybenzoate hydroxylase (EC 1.14.13.2), at 489,238 to 490,419
GFF1173
GFF1174 - Transcriptional regulator, IclR family, at 490,561 to 491,331
GFF1174
GFF1175 - Two-component system response regulator QseB, at 491,395 to 492,054
GFF1175
Position (kb)
489
490
491 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 488.563 kb on + strand, within GFF1171 at 488.563 kb on + strand, within GFF1171 at 488.563 kb on + strand, within GFF1171 at 488.564 kb on - strand, within GFF1171 at 488.564 kb on - strand, within GFF1171 at 488.564 kb on - strand, within GFF1171 at 488.564 kb on - strand, within GFF1171 at 489.053 kb on + strand, within GFF1172 at 489.053 kb on + strand, within GFF1172 at 489.053 kb on + strand, within GFF1172 at 489.054 kb on - strand, within GFF1172 at 489.054 kb on - strand, within GFF1172 at 489.054 kb on - strand, within GFF1172 at 489.054 kb on - strand, within GFF1172 at 489.054 kb on - strand, within GFF1172 at 489.173 kb on + strand, within GFF1172 at 489.173 kb on + strand, within GFF1172 at 489.173 kb on + strand, within GFF1172 at 489.173 kb on + strand, within GFF1172 at 489.174 kb on - strand, within GFF1172 at 489.174 kb on - strand, within GFF1172 at 489.174 kb on - strand, within GFF1172 at 489.239 kb on + strand at 489.240 kb on - strand at 489.240 kb on - strand at 489.240 kb on - strand at 489.240 kb on - strand at 489.240 kb on - strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.266 kb on + strand at 489.267 kb on - strand at 489.267 kb on - strand at 489.267 kb on - strand at 489.267 kb on - strand at 489.311 kb on + strand at 489.470 kb on + strand, within GFF1173 at 489.471 kb on - strand, within GFF1173 at 489.471 kb on - strand, within GFF1173 at 489.497 kb on + strand, within GFF1173 at 489.497 kb on + strand, within GFF1173 at 489.497 kb on + strand, within GFF1173 at 489.497 kb on + strand, within GFF1173 at 489.498 kb on - strand, within GFF1173 at 489.755 kb on + strand, within GFF1173 at 489.755 kb on + strand, within GFF1173 at 489.755 kb on + strand, within GFF1173 at 489.755 kb on + strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.756 kb on - strand, within GFF1173 at 489.758 kb on + strand, within GFF1173 at 489.758 kb on + strand, within GFF1173 at 489.759 kb on - strand, within GFF1173 at 489.818 kb on + strand, within GFF1173 at 489.818 kb on + strand, within GFF1173 at 489.878 kb on + strand, within GFF1173 at 489.890 kb on + strand, within GFF1173 at 489.890 kb on + strand, within GFF1173 at 489.891 kb on - strand, within GFF1173 at 489.938 kb on + strand, within GFF1173 at 489.998 kb on + strand, within GFF1173 at 490.055 kb on + strand, within GFF1173 at 490.055 kb on + strand, within GFF1173 at 490.056 kb on - strand, within GFF1173 at 490.056 kb on - strand, within GFF1173 at 490.121 kb on + strand, within GFF1173 at 490.121 kb on + strand, within GFF1173 at 490.122 kb on - strand, within GFF1173 at 490.307 kb on + strand at 490.307 kb on + strand at 490.307 kb on + strand at 490.308 kb on - strand at 490.308 kb on - strand at 490.308 kb on - strand at 490.308 kb on - strand at 490.452 kb on - strand at 490.729 kb on + strand, within GFF1174 at 490.750 kb on + strand, within GFF1174 at 490.750 kb on + strand, within GFF1174 at 490.792 kb on + strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.793 kb on - strand, within GFF1174 at 490.925 kb on - strand, within GFF1174 at 490.925 kb on - strand, within GFF1174 at 490.925 kb on - strand, within GFF1174 at 490.951 kb on + strand, within GFF1174 at 491.140 kb on + strand, within GFF1174 at 491.140 kb on + strand, within GFF1174 at 491.140 kb on + strand, within GFF1174 at 491.141 kb on - strand, within GFF1174 at 491.254 kb on + strand, within GFF1174 at 491.291 kb on + strand at 491.292 kb on - strand at 491.354 kb on - strand at 491.411 kb on + strand at 491.411 kb on + strand at 491.412 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 488,563 + GFF1171 0.71 +0.3 488,563 + GFF1171 0.71 +0.0 488,563 + GFF1171 0.71 -0.1 488,564 - GFF1171 0.71 -0.5 488,564 - GFF1171 0.71 +0.3 488,564 - GFF1171 0.71 +1.1 488,564 - GFF1171 0.71 +0.0 489,053 + GFF1172 0.53 -0.7 489,053 + GFF1172 0.53 -0.8 489,053 + GFF1172 0.53 -2.1 489,054 - GFF1172 0.54 +0.2 489,054 - GFF1172 0.54 +0.7 489,054 - GFF1172 0.54 -2.0 489,054 - GFF1172 0.54 +0.3 489,054 - GFF1172 0.54 +1.1 489,173 + GFF1172 0.83 -1.0 489,173 + GFF1172 0.83 -0.1 489,173 + GFF1172 0.83 -0.0 489,173 + GFF1172 0.83 -0.3 489,174 - GFF1172 0.83 +1.3 489,174 - GFF1172 0.83 +0.0 489,174 - GFF1172 0.83 -0.1 489,239 + +0.1 489,240 - +0.5 489,240 - -1.2 489,240 - +1.9 489,240 - +0.1 489,240 - -1.0 489,266 + +0.1 489,266 + +1.0 489,266 + +0.1 489,266 + +0.1 489,266 + -1.1 489,266 + -0.2 489,266 + -0.6 489,267 - -1.3 489,267 - +1.5 489,267 - -0.8 489,267 - -1.2 489,311 + +0.3 489,470 + GFF1173 0.20 -0.0 489,471 - GFF1173 0.20 -0.5 489,471 - GFF1173 0.20 -0.7 489,497 + GFF1173 0.22 +0.7 489,497 + GFF1173 0.22 +1.7 489,497 + GFF1173 0.22 -0.2 489,497 + GFF1173 0.22 +0.1 489,498 - GFF1173 0.22 +0.3 489,755 + GFF1173 0.44 -1.1 489,755 + GFF1173 0.44 -2.3 489,755 + GFF1173 0.44 -1.1 489,755 + GFF1173 0.44 -0.9 489,756 - GFF1173 0.44 -1.0 489,756 - GFF1173 0.44 +1.7 489,756 - GFF1173 0.44 -0.9 489,756 - GFF1173 0.44 -0.0 489,756 - GFF1173 0.44 -0.3 489,756 - GFF1173 0.44 -0.7 489,756 - GFF1173 0.44 -0.5 489,756 - GFF1173 0.44 -1.5 489,756 - GFF1173 0.44 -0.5 489,756 - GFF1173 0.44 +3.7 489,756 - GFF1173 0.44 -0.1 489,756 - GFF1173 0.44 -1.4 489,758 + GFF1173 0.44 +0.3 489,758 + GFF1173 0.44 -0.4 489,759 - GFF1173 0.44 -0.3 489,818 + GFF1173 0.49 -1.8 489,818 + GFF1173 0.49 -0.5 489,878 + GFF1173 0.54 -0.3 489,890 + GFF1173 0.55 +0.9 489,890 + GFF1173 0.55 -0.3 489,891 - GFF1173 0.55 -1.0 489,938 + GFF1173 0.59 -0.7 489,998 + GFF1173 0.64 -1.1 490,055 + GFF1173 0.69 -1.0 490,055 + GFF1173 0.69 -1.5 490,056 - GFF1173 0.69 +0.2 490,056 - GFF1173 0.69 -0.5 490,121 + GFF1173 0.75 -0.1 490,121 + GFF1173 0.75 +0.8 490,122 - GFF1173 0.75 -0.4 490,307 + -0.8 490,307 + +0.3 490,307 + -0.9 490,308 - +0.7 490,308 - -1.0 490,308 - -0.8 490,308 - +0.4 490,452 - -2.5 490,729 + GFF1174 0.22 -1.5 490,750 + GFF1174 0.25 +0.1 490,750 + GFF1174 0.25 +0.3 490,792 + GFF1174 0.30 -1.5 490,793 - GFF1174 0.30 -0.1 490,793 - GFF1174 0.30 -0.5 490,793 - GFF1174 0.30 +0.3 490,793 - GFF1174 0.30 -0.3 490,793 - GFF1174 0.30 +1.2 490,793 - GFF1174 0.30 -0.7 490,793 - GFF1174 0.30 -0.7 490,925 - GFF1174 0.47 +0.2 490,925 - GFF1174 0.47 +1.3 490,925 - GFF1174 0.47 -0.7 490,951 + GFF1174 0.51 +0.6 491,140 + GFF1174 0.75 +0.8 491,140 + GFF1174 0.75 +0.9 491,140 + GFF1174 0.75 +0.2 491,141 - GFF1174 0.75 -0.3 491,254 + GFF1174 0.90 -1.8 491,291 + +0.5 491,292 - +0.3 491,354 - -0.2 491,411 + -0.5 491,411 + +0.5 491,412 - -0.4
Or see this region's nucleotide sequence