Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF111 and GFF112 overlap by 4 nucleotides GFF112 and GFF113 are separated by 28 nucleotides GFF113 and GFF114 overlap by 11 nucleotides
GFF111 - hypothetical protein, at 140,196 to 141,221
GFF111
GFF112 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 141,218 to 141,994
GFF112
GFF113 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 142,023 to 142,742
GFF113
GFF114 - ABC transporter, permease protein (cluster 3, basic aa/glutamine/opines), at 142,732 to 143,388
GFF114
Position (kb) 141
142 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 140.278 kb on + strand at 140.278 kb on + strand at 140.278 kb on + strand at 140.279 kb on - strand at 140.279 kb on - strand at 140.404 kb on + strand, within GFF111 at 140.404 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.794 kb on + strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 140.795 kb on - strand, within GFF111 at 141.028 kb on + strand, within GFF111 at 141.351 kb on + strand, within GFF112 at 141.351 kb on + strand, within GFF112 at 141.351 kb on + strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.352 kb on - strand, within GFF112 at 141.627 kb on + strand, within GFF112 at 141.627 kb on + strand, within GFF112 at 141.627 kb on + strand, within GFF112 at 141.627 kb on + strand, within GFF112 at 141.628 kb on - strand, within GFF112 at 141.639 kb on + strand, within GFF112 at 141.639 kb on + strand, within GFF112 at 141.640 kb on - strand, within GFF112 at 141.640 kb on - strand, within GFF112 at 141.651 kb on + strand, within GFF112 at 141.652 kb on - strand, within GFF112 at 141.738 kb on + strand, within GFF112 at 141.739 kb on - strand, within GFF112 at 142.698 kb on + strand at 142.698 kb on + strand at 142.698 kb on + strand at 142.698 kb on + strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.699 kb on - strand at 142.808 kb on + strand, within GFF114 at 142.808 kb on + strand, within GFF114 at 142.809 kb on - strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.940 kb on + strand, within GFF114 at 142.941 kb on - strand, within GFF114 at 142.941 kb on - strand, within GFF114
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 140,278 + -1.3 140,278 + -1.4 140,278 + -0.0 140,279 - +1.0 140,279 - +0.3 140,404 + GFF111 0.20 +0.5 140,404 + GFF111 0.20 +0.1 140,794 + GFF111 0.58 +0.2 140,794 + GFF111 0.58 -0.0 140,795 - GFF111 0.58 +1.0 140,795 - GFF111 0.58 +0.9 140,795 - GFF111 0.58 -0.0 140,795 - GFF111 0.58 -0.0 141,028 + GFF111 0.81 +0.0 141,351 + GFF112 0.17 -0.7 141,351 + GFF112 0.17 +2.9 141,351 + GFF112 0.17 -2.0 141,352 - GFF112 0.17 -1.3 141,352 - GFF112 0.17 -0.7 141,352 - GFF112 0.17 -1.3 141,352 - GFF112 0.17 -0.9 141,352 - GFF112 0.17 -0.2 141,352 - GFF112 0.17 -0.1 141,627 + GFF112 0.53 -0.4 141,627 + GFF112 0.53 +0.4 141,627 + GFF112 0.53 -0.7 141,627 + GFF112 0.53 +1.3 141,628 - GFF112 0.53 -0.3 141,639 + GFF112 0.54 +0.0 141,639 + GFF112 0.54 -0.6 141,640 - GFF112 0.54 +0.2 141,640 - GFF112 0.54 -0.9 141,651 + GFF112 0.56 -2.1 141,652 - GFF112 0.56 +0.1 141,738 + GFF112 0.67 -0.3 141,739 - GFF112 0.67 -0.1 142,698 + +0.3 142,698 + -0.6 142,698 + -0.1 142,698 + -1.0 142,699 - +0.2 142,699 - -1.4 142,699 - -1.0 142,699 - +0.4 142,699 - +0.6 142,699 - -0.0 142,699 - -0.4 142,808 + GFF114 0.12 -0.6 142,808 + GFF114 0.12 -0.9 142,809 - GFF114 0.12 +0.6 142,940 + GFF114 0.32 -0.3 142,940 + GFF114 0.32 +0.5 142,940 + GFF114 0.32 +0.4 142,940 + GFF114 0.32 -0.2 142,940 + GFF114 0.32 -1.5 142,941 - GFF114 0.32 -2.6 142,941 - GFF114 0.32 +0.3
Or see this region's nucleotide sequence