Strain Fitness in Variovorax sp. SCN45 around GFF111

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF109 and GFF110 are separated by 10 nucleotidesGFF110 and GFF111 overlap by 4 nucleotidesGFF111 and GFF112 overlap by 4 nucleotidesGFF112 and GFF113 are separated by 28 nucleotides GFF109 - Protein lysine acetyltransferase Pat (EC 2.3.1.-), at 136,527 to 139,229 GFF109 GFF110 - pyridoxal-phosphate dependent enzyme family protein, at 139,240 to 140,199 GFF110 GFF111 - hypothetical protein, at 140,196 to 141,221 GFF111 GFF112 - ABC transporter, substrate-binding protein (cluster 3, basic aa/glutamine/opines), at 141,218 to 141,994 GFF112 GFF113 - ABC transporter, ATP-binding protein (cluster 3, basic aa/glutamine/opines), at 142,023 to 142,742 GFF113 Position (kb) 140 141 142Strain fitness (log2 ratio) -2 -1 0 1 2 3at 139.218 kb on + strandat 139.218 kb on + strandat 139.218 kb on + strandat 139.218 kb on + strandat 139.218 kb on + strandat 139.219 kb on - strandat 139.219 kb on - strandat 139.219 kb on - strandat 139.219 kb on - strandat 139.219 kb on - strandat 139.227 kb on + strandat 139.442 kb on + strand, within GFF110at 139.442 kb on + strand, within GFF110at 139.443 kb on - strand, within GFF110at 139.443 kb on - strand, within GFF110at 139.553 kb on + strand, within GFF110at 139.553 kb on + strand, within GFF110at 139.553 kb on + strand, within GFF110at 139.553 kb on + strand, within GFF110at 139.554 kb on - strand, within GFF110at 139.745 kb on + strand, within GFF110at 139.746 kb on - strand, within GFF110at 139.746 kb on - strand, within GFF110at 139.746 kb on - strand, within GFF110at 139.746 kb on - strand, within GFF110at 139.928 kb on + strand, within GFF110at 139.928 kb on + strand, within GFF110at 139.928 kb on + strand, within GFF110at 139.929 kb on - strand, within GFF110at 140.121 kb on + strandat 140.132 kb on + strandat 140.132 kb on + strandat 140.278 kb on + strandat 140.278 kb on + strandat 140.278 kb on + strandat 140.279 kb on - strandat 140.279 kb on - strandat 140.404 kb on + strand, within GFF111at 140.404 kb on + strand, within GFF111at 140.794 kb on + strand, within GFF111at 140.794 kb on + strand, within GFF111at 140.795 kb on - strand, within GFF111at 140.795 kb on - strand, within GFF111at 140.795 kb on - strand, within GFF111at 140.795 kb on - strand, within GFF111at 141.028 kb on + strand, within GFF111at 141.351 kb on + strand, within GFF112at 141.351 kb on + strand, within GFF112at 141.351 kb on + strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.352 kb on - strand, within GFF112at 141.627 kb on + strand, within GFF112at 141.627 kb on + strand, within GFF112at 141.627 kb on + strand, within GFF112at 141.627 kb on + strand, within GFF112at 141.628 kb on - strand, within GFF112at 141.639 kb on + strand, within GFF112at 141.639 kb on + strand, within GFF112at 141.640 kb on - strand, within GFF112at 141.640 kb on - strand, within GFF112at 141.651 kb on + strand, within GFF112at 141.652 kb on - strand, within GFF112at 141.738 kb on + strand, within GFF112at 141.739 kb on - strand, within GFF112

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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139,218 + +0.4
139,218 + +0.3
139,218 + +1.3
139,218 + -0.2
139,218 + +0.4
139,219 - -0.3
139,219 - +0.1
139,219 - -0.9
139,219 - +1.3
139,219 - -0.1
139,227 + +1.3
139,442 + GFF110 0.21 -0.4
139,442 + GFF110 0.21 -0.4
139,443 - GFF110 0.21 -0.4
139,443 - GFF110 0.21 +0.6
139,553 + GFF110 0.33 -1.5
139,553 + GFF110 0.33 -0.0
139,553 + GFF110 0.33 -0.3
139,553 + GFF110 0.33 +2.3
139,554 - GFF110 0.33 -0.5
139,745 + GFF110 0.53 -0.1
139,746 - GFF110 0.53 -0.1
139,746 - GFF110 0.53 -0.2
139,746 - GFF110 0.53 -0.8
139,746 - GFF110 0.53 -0.7
139,928 + GFF110 0.72 -0.3
139,928 + GFF110 0.72 -0.0
139,928 + GFF110 0.72 +2.3
139,929 - GFF110 0.72 -1.3
140,121 + +0.9
140,132 + -0.5
140,132 + -0.4
140,278 + -1.3
140,278 + -1.4
140,278 + -0.0
140,279 - +1.0
140,279 - +0.3
140,404 + GFF111 0.20 +0.5
140,404 + GFF111 0.20 +0.1
140,794 + GFF111 0.58 +0.2
140,794 + GFF111 0.58 -0.0
140,795 - GFF111 0.58 +1.0
140,795 - GFF111 0.58 +0.9
140,795 - GFF111 0.58 -0.0
140,795 - GFF111 0.58 -0.0
141,028 + GFF111 0.81 +0.0
141,351 + GFF112 0.17 -0.7
141,351 + GFF112 0.17 +2.9
141,351 + GFF112 0.17 -2.0
141,352 - GFF112 0.17 -1.3
141,352 - GFF112 0.17 -0.7
141,352 - GFF112 0.17 -1.3
141,352 - GFF112 0.17 -0.9
141,352 - GFF112 0.17 -0.2
141,352 - GFF112 0.17 -0.1
141,627 + GFF112 0.53 -0.4
141,627 + GFF112 0.53 +0.4
141,627 + GFF112 0.53 -0.7
141,627 + GFF112 0.53 +1.3
141,628 - GFF112 0.53 -0.3
141,639 + GFF112 0.54 +0.0
141,639 + GFF112 0.54 -0.6
141,640 - GFF112 0.54 +0.2
141,640 - GFF112 0.54 -0.9
141,651 + GFF112 0.56 -2.1
141,652 - GFF112 0.56 +0.1
141,738 + GFF112 0.67 -0.3
141,739 - GFF112 0.67 -0.1

Or see this region's nucleotide sequence