Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1050 and GFF1051 are separated by 15 nucleotides GFF1051 and GFF1052 overlap by 4 nucleotides GFF1052 and GFF1053 are separated by 118 nucleotides GFF1053 and GFF1054 are separated by 137 nucleotides
GFF1050 - Transcriptional regulator, LysR family, at 365,929 to 366,780
GFF1050
GFF1051 - no description, at 366,796 to 366,921
GFF1051
GFF1052 - hypothetical protein, at 366,918 to 367,490
GFF1052
GFF1053 - no description, at 367,609 to 368,298
GFF1053
GFF1054 - Catechol 2,3-dioxygenase (EC 1.13.11.2), at 368,436 to 369,419
GFF1054
Position (kb)
366
367
368 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 365.927 kb on - strand at 366.797 kb on + strand at 366.798 kb on - strand at 366.841 kb on + strand, within GFF1051 at 366.842 kb on - strand, within GFF1051 at 366.910 kb on + strand at 366.910 kb on + strand at 366.910 kb on + strand at 366.910 kb on + strand at 366.910 kb on + strand at 366.911 kb on - strand at 366.911 kb on - strand at 366.911 kb on - strand at 366.911 kb on - strand at 366.952 kb on + strand at 366.952 kb on + strand at 366.952 kb on + strand at 366.952 kb on + strand at 366.952 kb on + strand at 366.952 kb on + strand at 366.953 kb on - strand at 366.953 kb on - strand at 366.953 kb on - strand at 366.953 kb on - strand at 366.964 kb on + strand at 366.964 kb on + strand at 366.965 kb on - strand at 366.965 kb on - strand at 366.965 kb on - strand at 367.159 kb on + strand, within GFF1052 at 367.159 kb on + strand, within GFF1052 at 367.312 kb on + strand, within GFF1052 at 367.313 kb on - strand, within GFF1052 at 367.313 kb on - strand, within GFF1052 at 367.313 kb on - strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.324 kb on + strand, within GFF1052 at 367.325 kb on - strand, within GFF1052 at 367.325 kb on - strand, within GFF1052 at 367.325 kb on - strand, within GFF1052 at 367.325 kb on - strand, within GFF1052 at 367.450 kb on + strand at 367.450 kb on + strand at 367.450 kb on + strand at 367.474 kb on + strand at 367.474 kb on + strand at 367.475 kb on - strand at 367.475 kb on - strand at 367.475 kb on - strand at 367.475 kb on - strand at 367.566 kb on - strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.567 kb on + strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.568 kb on - strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.610 kb on + strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.611 kb on - strand at 367.793 kb on + strand, within GFF1053 at 367.811 kb on + strand, within GFF1053 at 367.811 kb on + strand, within GFF1053 at 367.811 kb on + strand, within GFF1053 at 367.812 kb on - strand, within GFF1053 at 367.812 kb on - strand, within GFF1053 at 367.812 kb on - strand, within GFF1053 at 367.812 kb on - strand, within GFF1053 at 368.029 kb on + strand, within GFF1053 at 368.029 kb on + strand, within GFF1053 at 368.029 kb on + strand, within GFF1053 at 368.257 kb on + strand at 368.257 kb on + strand at 368.257 kb on + strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.258 kb on - strand at 368.334 kb on + strand at 368.334 kb on + strand at 368.334 kb on + strand at 368.334 kb on + strand at 368.335 kb on - strand at 368.335 kb on - strand at 368.335 kb on - strand at 368.357 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 365,927 - -0.3 366,797 + -0.1 366,798 - -0.7 366,841 + GFF1051 0.36 +1.1 366,842 - GFF1051 0.37 -0.0 366,910 + -0.6 366,910 + -1.2 366,910 + +0.7 366,910 + +0.2 366,910 + +0.0 366,911 - -0.4 366,911 - +0.7 366,911 - -0.2 366,911 - -1.3 366,952 + +1.1 366,952 + -0.1 366,952 + -0.3 366,952 + -0.8 366,952 + -0.7 366,952 + +0.6 366,953 - -1.2 366,953 - +0.6 366,953 - -1.1 366,953 - -0.8 366,964 + -0.9 366,964 + +0.2 366,965 - +1.0 366,965 - -0.7 366,965 - +1.0 367,159 + GFF1052 0.42 -0.3 367,159 + GFF1052 0.42 +0.7 367,312 + GFF1052 0.69 +0.7 367,313 - GFF1052 0.69 +0.2 367,313 - GFF1052 0.69 -0.7 367,313 - GFF1052 0.69 -0.6 367,324 + GFF1052 0.71 -0.6 367,324 + GFF1052 0.71 +0.4 367,324 + GFF1052 0.71 -0.1 367,324 + GFF1052 0.71 -0.3 367,324 + GFF1052 0.71 +1.4 367,324 + GFF1052 0.71 -1.8 367,324 + GFF1052 0.71 +1.1 367,324 + GFF1052 0.71 -0.0 367,325 - GFF1052 0.71 -0.8 367,325 - GFF1052 0.71 +0.4 367,325 - GFF1052 0.71 +0.2 367,325 - GFF1052 0.71 -0.2 367,450 + -0.6 367,450 + -0.5 367,450 + +0.3 367,474 + +0.7 367,474 + -0.9 367,475 - -3.7 367,475 - +0.6 367,475 - -0.8 367,475 - +0.5 367,566 - -1.9 367,567 + -1.6 367,567 + +0.6 367,567 + +0.2 367,567 + -0.5 367,567 + -2.3 367,567 + -0.8 367,567 + +0.3 367,567 + -0.9 367,567 + +0.1 367,567 + +0.7 367,567 + -0.5 367,567 + +0.1 367,567 + -1.8 367,567 + -0.1 367,567 + -0.4 367,567 + -0.2 367,567 + -1.8 367,568 - -0.9 367,568 - -0.5 367,568 - -0.6 367,568 - -0.8 367,568 - +1.0 367,568 - +1.9 367,568 - -0.8 367,568 - -0.0 367,568 - -0.1 367,568 - +1.1 367,568 - -0.0 367,568 - +2.4 367,568 - +0.6 367,568 - +0.1 367,610 + +0.5 367,610 + -0.1 367,610 + +0.6 367,610 + -0.6 367,610 + -1.8 367,610 + +0.8 367,610 + +0.5 367,610 + +0.5 367,611 - +0.2 367,611 - -1.3 367,611 - -1.1 367,611 - -0.1 367,611 - +0.1 367,611 - +0.2 367,611 - -1.6 367,611 - -1.7 367,611 - -0.4 367,611 - +1.4 367,793 + GFF1053 0.27 -0.1 367,811 + GFF1053 0.29 +0.2 367,811 + GFF1053 0.29 +0.2 367,811 + GFF1053 0.29 -0.6 367,812 - GFF1053 0.29 -1.2 367,812 - GFF1053 0.29 -0.5 367,812 - GFF1053 0.29 +0.6 367,812 - GFF1053 0.29 -0.6 368,029 + GFF1053 0.61 -0.5 368,029 + GFF1053 0.61 -0.4 368,029 + GFF1053 0.61 -0.6 368,257 + +1.7 368,257 + +0.3 368,257 + -0.2 368,258 - +0.5 368,258 - -0.7 368,258 - +0.3 368,258 - +0.2 368,258 - -1.2 368,258 - +1.4 368,258 - -1.9 368,334 + -1.0 368,334 + -1.2 368,334 + -0.7 368,334 + -0.6 368,335 - -0.2 368,335 - -0.6 368,335 - +0.0 368,357 + -0.4
Or see this region's nucleotide sequence