Strain Fitness in Escherichia coli ECRC62 around BNILDI_19195

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntndh and ycfP are separated by 399 nucleotidesycfP and nagZ are separated by 22 nucleotidesnagZ and thiK are separated by 10 nucleotidesthiK and lpoB overlap by 20 nucleotides BNILDI_19185: ndh - NADH-quinone dehydrogenase, at 3,858,101 to 3,859,405 ndh BNILDI_19190: ycfP - alpha/beta hydrolase YcfP, at 3,859,805 to 3,860,347 ycfP BNILDI_19195: nagZ - beta-N-acetylhexosaminidase, at 3,860,370 to 3,861,395 nagZ BNILDI_19200: thiK - thiamine kinase, at 3,861,406 to 3,862,230 thiK BNILDI_19205: lpoB - penicillin-binding protein activator LpoB, at 3,862,211 to 3,862,852 lpoB Position (kb) 3860 3861 3862Strain fitness (log2 ratio) -2 -1 0 1 2 3at 3859.660 kb on + strandat 3859.687 kb on + strandat 3859.785 kb on - strandat 3859.807 kb on - strandat 3859.853 kb on - strandat 3859.899 kb on + strand, within ycfPat 3860.221 kb on + strand, within ycfPat 3860.221 kb on + strand, within ycfPat 3860.222 kb on - strand, within ycfPat 3860.242 kb on + strand, within ycfPat 3860.247 kb on + strand, within ycfPat 3860.253 kb on - strand, within ycfPat 3860.261 kb on + strand, within ycfPat 3860.261 kb on + strand, within ycfPat 3860.262 kb on - strand, within ycfPat 3860.289 kb on - strand, within ycfPat 3860.317 kb on + strandat 3860.318 kb on - strandat 3860.337 kb on + strandat 3860.338 kb on - strandat 3860.338 kb on - strandat 3860.341 kb on + strandat 3860.342 kb on - strandat 3860.360 kb on + strandat 3860.360 kb on + strandat 3860.360 kb on + strandat 3860.372 kb on - strandat 3860.406 kb on + strandat 3860.407 kb on - strandat 3860.407 kb on - strandat 3860.407 kb on - strandat 3860.525 kb on + strand, within nagZat 3860.530 kb on - strand, within nagZat 3860.615 kb on - strand, within nagZat 3860.625 kb on - strand, within nagZat 3860.750 kb on - strand, within nagZat 3860.750 kb on - strand, within nagZat 3860.816 kb on + strand, within nagZat 3860.816 kb on + strand, within nagZat 3860.816 kb on + strand, within nagZat 3860.939 kb on + strand, within nagZat 3860.940 kb on - strand, within nagZat 3861.021 kb on - strand, within nagZat 3861.021 kb on - strand, within nagZat 3861.054 kb on - strand, within nagZat 3861.214 kb on - strand, within nagZat 3861.294 kb on - strandat 3861.337 kb on - strandat 3861.415 kb on + strandat 3861.421 kb on + strandat 3861.454 kb on - strandat 3861.548 kb on - strand, within thiKat 3861.581 kb on + strand, within thiKat 3861.582 kb on - strand, within thiKat 3861.582 kb on - strand, within thiKat 3861.706 kb on + strand, within thiKat 3861.729 kb on + strand, within thiKat 3861.730 kb on - strand, within thiKat 3861.743 kb on - strand, within thiKat 3861.784 kb on + strand, within thiKat 3861.892 kb on + strand, within thiKat 3861.892 kb on + strand, within thiKat 3861.896 kb on + strand, within thiKat 3861.896 kb on + strand, within thiKat 3861.897 kb on - strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.899 kb on + strand, within thiKat 3861.987 kb on - strand, within thiKat 3861.988 kb on + strand, within thiKat 3862.143 kb on + strand, within thiKat 3862.147 kb on - strand, within thiKat 3862.213 kb on - strandat 3862.213 kb on - strandat 3862.235 kb on - strandat 3862.359 kb on - strand, within lpoBat 3862.380 kb on - strand, within lpoB

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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3,859,660 + +0.4
3,859,687 + -0.4
3,859,785 - +0.0
3,859,807 - -0.7
3,859,853 - +0.5
3,859,899 + ycfP BNILDI_19190 0.17 +0.6
3,860,221 + ycfP BNILDI_19190 0.77 +0.3
3,860,221 + ycfP BNILDI_19190 0.77 -0.2
3,860,222 - ycfP BNILDI_19190 0.77 +0.3
3,860,242 + ycfP BNILDI_19190 0.80 +0.1
3,860,247 + ycfP BNILDI_19190 0.81 -0.7
3,860,253 - ycfP BNILDI_19190 0.83 +0.5
3,860,261 + ycfP BNILDI_19190 0.84 +0.7
3,860,261 + ycfP BNILDI_19190 0.84 -0.3
3,860,262 - ycfP BNILDI_19190 0.84 +0.5
3,860,289 - ycfP BNILDI_19190 0.89 +0.3
3,860,317 + -0.7
3,860,318 - -0.0
3,860,337 + -1.6
3,860,338 - -0.7
3,860,338 - -0.4
3,860,341 + +1.6
3,860,342 - +0.6
3,860,360 + -1.9
3,860,360 + +0.6
3,860,360 + +0.1
3,860,372 - -0.7
3,860,406 + +0.0
3,860,407 - +0.3
3,860,407 - -0.0
3,860,407 - -0.4
3,860,525 + nagZ BNILDI_19195 0.15 +0.2
3,860,530 - nagZ BNILDI_19195 0.16 +0.6
3,860,615 - nagZ BNILDI_19195 0.24 -0.9
3,860,625 - nagZ BNILDI_19195 0.25 +0.3
3,860,750 - nagZ BNILDI_19195 0.37 +0.7
3,860,750 - nagZ BNILDI_19195 0.37 +0.2
3,860,816 + nagZ BNILDI_19195 0.43 +2.0
3,860,816 + nagZ BNILDI_19195 0.43 +0.4
3,860,816 + nagZ BNILDI_19195 0.43 -0.4
3,860,939 + nagZ BNILDI_19195 0.55 -0.6
3,860,940 - nagZ BNILDI_19195 0.56 -0.4
3,861,021 - nagZ BNILDI_19195 0.63 -1.2
3,861,021 - nagZ BNILDI_19195 0.63 +0.0
3,861,054 - nagZ BNILDI_19195 0.67 -0.1
3,861,214 - nagZ BNILDI_19195 0.82 -1.1
3,861,294 - -0.3
3,861,337 - +0.6
3,861,415 + +0.1
3,861,421 + +3.0
3,861,454 - +3.2
3,861,548 - thiK BNILDI_19200 0.17 +0.2
3,861,581 + thiK BNILDI_19200 0.21 +0.2
3,861,582 - thiK BNILDI_19200 0.21 -0.4
3,861,582 - thiK BNILDI_19200 0.21 -1.1
3,861,706 + thiK BNILDI_19200 0.36 -0.7
3,861,729 + thiK BNILDI_19200 0.39 +0.6
3,861,730 - thiK BNILDI_19200 0.39 +0.0
3,861,743 - thiK BNILDI_19200 0.41 -0.4
3,861,784 + thiK BNILDI_19200 0.46 +1.6
3,861,892 + thiK BNILDI_19200 0.59 -0.2
3,861,892 + thiK BNILDI_19200 0.59 +2.9
3,861,896 + thiK BNILDI_19200 0.59 -0.2
3,861,896 + thiK BNILDI_19200 0.59 +0.0
3,861,897 - thiK BNILDI_19200 0.60 +0.2
3,861,899 + thiK BNILDI_19200 0.60 -0.1
3,861,899 + thiK BNILDI_19200 0.60 +0.1
3,861,899 + thiK BNILDI_19200 0.60 -1.2
3,861,987 - thiK BNILDI_19200 0.70 -0.6
3,861,988 + thiK BNILDI_19200 0.71 +0.6
3,862,143 + thiK BNILDI_19200 0.89 -1.2
3,862,147 - thiK BNILDI_19200 0.90 -0.6
3,862,213 - +0.1
3,862,213 - +0.1
3,862,235 - -0.6
3,862,359 - lpoB BNILDI_19205 0.23 +0.0
3,862,380 - lpoB BNILDI_19205 0.26 +0.1

Or see this region's nucleotide sequence