Strain Fitness in Escherichia coli ECRC62 around BNILDI_13545

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntarnB and ais are separated by 289 nucleotidesais and nudI are separated by 38 nucleotidesnudI and yfaZ are separated by 278 nucleotidesyfaZ and yfaY are separated by 99 nucleotides BNILDI_13535: arnB - UDP-4-amino-4-deoxy-L-arabinose aminotransferase, at 2,713,574 to 2,714,731 arnB BNILDI_13540: ais - Lipopolysaccharide core heptose(II)-phosphate phosphatase, at 2,715,021 to 2,715,623 ais BNILDI_13545: nudI - nucleoside triphosphatase NudI, at 2,715,662 to 2,716,087 nudI BNILDI_13550: yfaZ - Outer membrane protein YfaZ, at 2,716,366 to 2,716,908 yfaZ BNILDI_13555: yfaY - nicotinamide mononucleotide deamidase-related protein YfaY, at 2,717,008 to 2,718,210 yfaY Position (kb) 2715 2716 2717Strain fitness (log2 ratio) -2 -1 0 1at 2714.750 kb on + strandat 2715.073 kb on + strandat 2715.141 kb on - strand, within aisat 2715.149 kb on + strand, within aisat 2715.255 kb on - strand, within aisat 2715.257 kb on + strand, within aisat 2715.263 kb on + strand, within aisat 2715.339 kb on - strand, within aisat 2715.342 kb on - strand, within aisat 2715.397 kb on + strand, within aisat 2715.510 kb on + strand, within aisat 2715.510 kb on + strand, within aisat 2715.513 kb on - strand, within aisat 2715.811 kb on - strand, within nudIat 2715.846 kb on + strand, within nudIat 2716.192 kb on - strandat 2716.459 kb on + strand, within yfaZat 2716.460 kb on - strand, within yfaZat 2716.460 kb on - strand, within yfaZat 2716.527 kb on + strand, within yfaZat 2716.528 kb on - strand, within yfaZat 2716.618 kb on + strand, within yfaZat 2716.648 kb on + strand, within yfaZat 2716.718 kb on - strand, within yfaZat 2716.718 kb on - strand, within yfaZat 2716.721 kb on - strand, within yfaZat 2716.721 kb on - strand, within yfaZat 2716.852 kb on - strand, within yfaZat 2716.952 kb on + strandat 2716.976 kb on + strandat 2717.012 kb on + strandat 2717.013 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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2,714,750 + +0.6
2,715,073 + +0.8
2,715,141 - ais BNILDI_13540 0.20 -1.0
2,715,149 + ais BNILDI_13540 0.21 +1.3
2,715,255 - ais BNILDI_13540 0.39 +0.4
2,715,257 + ais BNILDI_13540 0.39 +0.7
2,715,263 + ais BNILDI_13540 0.40 +0.0
2,715,339 - ais BNILDI_13540 0.53 -2.5
2,715,342 - ais BNILDI_13540 0.53 +0.0
2,715,397 + ais BNILDI_13540 0.62 +0.2
2,715,510 + ais BNILDI_13540 0.81 +0.4
2,715,510 + ais BNILDI_13540 0.81 -0.7
2,715,513 - ais BNILDI_13540 0.82 +0.9
2,715,811 - nudI BNILDI_13545 0.35 -0.3
2,715,846 + nudI BNILDI_13545 0.43 +0.0
2,716,192 - +0.3
2,716,459 + yfaZ BNILDI_13550 0.17 +0.2
2,716,460 - yfaZ BNILDI_13550 0.17 -0.6
2,716,460 - yfaZ BNILDI_13550 0.17 +0.1
2,716,527 + yfaZ BNILDI_13550 0.30 +0.9
2,716,528 - yfaZ BNILDI_13550 0.30 -0.4
2,716,618 + yfaZ BNILDI_13550 0.46 -0.2
2,716,648 + yfaZ BNILDI_13550 0.52 +0.4
2,716,718 - yfaZ BNILDI_13550 0.65 -1.0
2,716,718 - yfaZ BNILDI_13550 0.65 -0.6
2,716,721 - yfaZ BNILDI_13550 0.65 -1.1
2,716,721 - yfaZ BNILDI_13550 0.65 -0.9
2,716,852 - yfaZ BNILDI_13550 0.90 +0.8
2,716,952 + +1.6
2,716,976 + +0.5
2,717,012 + +1.2
2,717,013 - +0.8

Or see this region's nucleotide sequence