Strain Fitness in Escherichia coli ECRC62 around BNILDI_12240

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nttnp-IS3 and tnp-IS3 overlap by 4 nucleotidestnp-IS3 and yfgG are separated by 46 nucleotidesyfgG and BNILDI_12245 are separated by 35 nucleotidesBNILDI_12245 and pdeF are separated by 181 nucleotides BNILDI_12230: tnp-IS3 - IS3 family transposase, at 2,455,671 to 2,456,537 tnp-IS3 BNILDI_12235: tnp-IS3 - IS3 family IS3 transposase, at 2,456,534 to 2,456,833 tnp-IS3 BNILDI_12240: yfgG - Uncharacterized protein YfgG, at 2,456,880 to 2,457,038 yfgG BNILDI_12245: BNILDI_12245 - hypothetical protein, at 2,457,074 to 2,457,226 _12245 BNILDI_12250: pdeF - cyclic-guanylate-specific phosphodiesterase PdeF, at 2,457,408 to 2,459,651 pdeF Position (kb) 2456 2457 2458Strain fitness (log2 ratio) -1 0 1at 2456.979 kb on - strand, within yfgGat 2457.073 kb on + strandat 2457.098 kb on + strand, within BNILDI_12245at 2457.164 kb on + strand, within BNILDI_12245at 2457.190 kb on - strand, within BNILDI_12245at 2457.224 kb on + strandat 2457.233 kb on - strandat 2457.273 kb on + strandat 2457.320 kb on + strandat 2457.369 kb on - strandat 2457.407 kb on + strandat 2457.408 kb on - strandat 2457.427 kb on - strandat 2457.504 kb on - strandat 2457.546 kb on + strandat 2457.726 kb on - strand, within pdeFat 2457.737 kb on + strand, within pdeFat 2457.781 kb on + strand, within pdeFat 2457.781 kb on + strand, within pdeFat 2457.794 kb on - strand, within pdeFat 2457.844 kb on + strand, within pdeFat 2457.912 kb on + strand, within pdeF

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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2,456,979 - yfgG BNILDI_12240 0.62 -0.1
2,457,073 + +0.5
2,457,098 + BNILDI_12245 0.16 +0.2
2,457,164 + BNILDI_12245 0.59 +0.4
2,457,190 - BNILDI_12245 0.76 +1.1
2,457,224 + +0.7
2,457,233 - +0.1
2,457,273 + +0.1
2,457,320 + -0.5
2,457,369 - +0.6
2,457,407 + +0.0
2,457,408 - +0.4
2,457,427 - +0.2
2,457,504 - -0.9
2,457,546 + +1.6
2,457,726 - pdeF BNILDI_12250 0.14 +0.3
2,457,737 + pdeF BNILDI_12250 0.15 +0.2
2,457,781 + pdeF BNILDI_12250 0.17 +1.6
2,457,781 + pdeF BNILDI_12250 0.17 -0.7
2,457,794 - pdeF BNILDI_12250 0.17 +0.2
2,457,844 + pdeF BNILDI_12250 0.19 -0.9
2,457,912 + pdeF BNILDI_12250 0.22 -1.2

Or see this region's nucleotide sequence