Strain Fitness in Escherichia coli ECRC62 around BNILDI_10085

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntguaD and xanQ are separated by 17 nucleotidesxanQ and xdhD are separated by 164 nucleotides BNILDI_10080: guaD - guanine deaminase, at 2,024,636 to 2,025,952 guaD BNILDI_10085: xanQ - xanthine/proton symporter XanQ, at 2,025,970 to 2,027,370 xanQ BNILDI_10090: xdhD - putative hypoxanthine oxidase XdhD, at 2,027,535 to 2,030,405 xdhD Position (kb) 2025 2026 2027 2028Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 2024.995 kb on + strand, within guaDat 2025.184 kb on - strand, within guaDat 2025.347 kb on + strand, within guaDat 2025.348 kb on - strand, within guaDat 2025.348 kb on - strand, within guaDat 2025.400 kb on - strand, within guaDat 2025.470 kb on - strand, within guaDat 2025.470 kb on - strand, within guaDat 2025.563 kb on + strand, within guaDat 2025.563 kb on + strand, within guaDat 2025.564 kb on - strand, within guaDat 2025.710 kb on - strand, within guaDat 2025.829 kb on + strandat 2025.829 kb on + strandat 2025.839 kb on + strandat 2025.839 kb on + strandat 2025.853 kb on + strandat 2025.965 kb on + strandat 2025.996 kb on + strandat 2025.997 kb on - strandat 2026.039 kb on - strandat 2026.041 kb on + strandat 2026.041 kb on + strandat 2026.042 kb on - strandat 2026.046 kb on - strandat 2026.077 kb on + strandat 2026.098 kb on - strandat 2026.176 kb on + strand, within xanQat 2026.176 kb on + strand, within xanQat 2026.176 kb on + strand, within xanQat 2026.177 kb on - strand, within xanQat 2026.177 kb on - strand, within xanQat 2026.213 kb on + strand, within xanQat 2026.420 kb on + strand, within xanQat 2026.493 kb on + strand, within xanQat 2026.493 kb on + strand, within xanQat 2026.494 kb on - strand, within xanQat 2026.622 kb on + strand, within xanQat 2026.623 kb on - strand, within xanQat 2026.768 kb on - strand, within xanQat 2026.787 kb on - strand, within xanQat 2026.795 kb on + strand, within xanQat 2026.830 kb on + strand, within xanQat 2026.831 kb on - strand, within xanQat 2026.932 kb on + strand, within xanQat 2026.945 kb on + strand, within xanQat 2026.945 kb on + strand, within xanQat 2026.960 kb on - strand, within xanQat 2027.024 kb on - strand, within xanQat 2027.080 kb on - strand, within xanQat 2027.114 kb on - strand, within xanQat 2027.114 kb on - strand, within xanQat 2027.143 kb on + strand, within xanQat 2027.143 kb on + strand, within xanQat 2027.144 kb on - strand, within xanQat 2027.157 kb on + strand, within xanQat 2027.157 kb on + strand, within xanQat 2027.184 kb on + strand, within xanQat 2027.195 kb on + strand, within xanQat 2027.196 kb on - strand, within xanQat 2027.221 kb on + strand, within xanQat 2027.335 kb on + strandat 2027.336 kb on - strandat 2027.337 kb on + strandat 2027.338 kb on - strandat 2027.385 kb on + strandat 2027.462 kb on - strandat 2027.485 kb on + strandat 2027.486 kb on - strandat 2027.486 kb on - strandat 2027.529 kb on + strandat 2027.533 kb on - strandat 2027.536 kb on + strandat 2027.537 kb on - strandat 2027.560 kb on - strandat 2027.647 kb on - strandat 2027.798 kb on - strandat 2027.816 kb on + strandat 2027.848 kb on + strand, within xdhDat 2027.996 kb on + strand, within xdhDat 2027.997 kb on - strand, within xdhDat 2027.997 kb on - strand, within xdhDat 2027.998 kb on + strand, within xdhDat 2028.069 kb on - strand, within xdhDat 2028.229 kb on + strand, within xdhD

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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2,024,995 + guaD BNILDI_10080 0.27 +0.3
2,025,184 - guaD BNILDI_10080 0.42 +0.3
2,025,347 + guaD BNILDI_10080 0.54 -0.3
2,025,348 - guaD BNILDI_10080 0.54 +0.1
2,025,348 - guaD BNILDI_10080 0.54 +0.7
2,025,400 - guaD BNILDI_10080 0.58 +0.1
2,025,470 - guaD BNILDI_10080 0.63 -2.1
2,025,470 - guaD BNILDI_10080 0.63 +0.0
2,025,563 + guaD BNILDI_10080 0.70 -0.8
2,025,563 + guaD BNILDI_10080 0.70 +0.0
2,025,564 - guaD BNILDI_10080 0.70 -0.5
2,025,710 - guaD BNILDI_10080 0.82 -0.2
2,025,829 + +1.7
2,025,829 + -2.2
2,025,839 + +0.6
2,025,839 + +0.6
2,025,853 + -1.6
2,025,965 + +1.0
2,025,996 + -0.1
2,025,997 - +0.0
2,026,039 - -0.1
2,026,041 + +0.1
2,026,041 + -0.1
2,026,042 - -0.6
2,026,046 - +0.6
2,026,077 + +0.6
2,026,098 - +0.1
2,026,176 + xanQ BNILDI_10085 0.15 -0.1
2,026,176 + xanQ BNILDI_10085 0.15 +0.4
2,026,176 + xanQ BNILDI_10085 0.15 +0.9
2,026,177 - xanQ BNILDI_10085 0.15 +0.3
2,026,177 - xanQ BNILDI_10085 0.15 -0.7
2,026,213 + xanQ BNILDI_10085 0.17 -0.0
2,026,420 + xanQ BNILDI_10085 0.32 -0.0
2,026,493 + xanQ BNILDI_10085 0.37 +0.7
2,026,493 + xanQ BNILDI_10085 0.37 +0.0
2,026,494 - xanQ BNILDI_10085 0.37 +1.1
2,026,622 + xanQ BNILDI_10085 0.47 +0.4
2,026,623 - xanQ BNILDI_10085 0.47 -0.5
2,026,768 - xanQ BNILDI_10085 0.57 -0.3
2,026,787 - xanQ BNILDI_10085 0.58 -0.0
2,026,795 + xanQ BNILDI_10085 0.59 -0.1
2,026,830 + xanQ BNILDI_10085 0.61 -0.9
2,026,831 - xanQ BNILDI_10085 0.61 +1.3
2,026,932 + xanQ BNILDI_10085 0.69 +0.3
2,026,945 + xanQ BNILDI_10085 0.70 +3.3
2,026,945 + xanQ BNILDI_10085 0.70 -1.0
2,026,960 - xanQ BNILDI_10085 0.71 +0.5
2,027,024 - xanQ BNILDI_10085 0.75 +0.0
2,027,080 - xanQ BNILDI_10085 0.79 +0.5
2,027,114 - xanQ BNILDI_10085 0.82 -0.1
2,027,114 - xanQ BNILDI_10085 0.82 +2.0
2,027,143 + xanQ BNILDI_10085 0.84 -1.1
2,027,143 + xanQ BNILDI_10085 0.84 -0.4
2,027,144 - xanQ BNILDI_10085 0.84 +0.4
2,027,157 + xanQ BNILDI_10085 0.85 +0.4
2,027,157 + xanQ BNILDI_10085 0.85 +1.0
2,027,184 + xanQ BNILDI_10085 0.87 +0.2
2,027,195 + xanQ BNILDI_10085 0.87 +0.1
2,027,196 - xanQ BNILDI_10085 0.88 -2.3
2,027,221 + xanQ BNILDI_10085 0.89 -0.5
2,027,335 + -4.5
2,027,336 - -0.0
2,027,337 + -0.3
2,027,338 - +0.5
2,027,385 + -1.7
2,027,462 - +0.4
2,027,485 + -0.5
2,027,486 - +0.3
2,027,486 - +0.3
2,027,529 + +0.3
2,027,533 - -2.3
2,027,536 + -0.7
2,027,537 - -0.7
2,027,560 - -0.5
2,027,647 - +1.2
2,027,798 - +1.0
2,027,816 + -0.6
2,027,848 + xdhD BNILDI_10090 0.11 +0.7
2,027,996 + xdhD BNILDI_10090 0.16 +0.3
2,027,997 - xdhD BNILDI_10090 0.16 +0.1
2,027,997 - xdhD BNILDI_10090 0.16 -0.7
2,027,998 + xdhD BNILDI_10090 0.16 -0.3
2,028,069 - xdhD BNILDI_10090 0.19 -1.1
2,028,229 + xdhD BNILDI_10090 0.24 -0.4

Or see this region's nucleotide sequence