Strain Fitness in Escherichia coli ECRC62 around BNILDI_09650

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmutY and trmB are separated by 160 nucleotidestrmB and yggL overlap by 1 nucleotidesyggL and yggN are separated by 183 nucleotides BNILDI_09645: mutY - A/G-specific adenine glycosylase, at 1,943,457 to 1,944,509 mutY BNILDI_09650: trmB - tRNA (guanosine(46)-N7)-methyltransferase TrmB, at 1,944,670 to 1,945,389 trmB BNILDI_09655: yggL - Uncharacterized protein YggL, at 1,945,389 to 1,945,715 yggL BNILDI_09660: yggN - Uncharacterized protein YggN, at 1,945,899 to 1,946,618 yggN Position (kb) 1944 1945 1946Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1943.736 kb on + strand, within mutYat 1943.737 kb on - strand, within mutYat 1943.737 kb on - strand, within mutYat 1943.778 kb on + strand, within mutYat 1943.778 kb on + strand, within mutYat 1943.778 kb on + strand, within mutYat 1943.779 kb on - strand, within mutYat 1943.885 kb on + strand, within mutYat 1943.885 kb on + strand, within mutYat 1943.886 kb on - strand, within mutYat 1943.970 kb on - strand, within mutYat 1944.018 kb on + strand, within mutYat 1944.064 kb on + strand, within mutYat 1944.065 kb on - strand, within mutYat 1944.213 kb on + strand, within mutYat 1944.263 kb on - strand, within mutYat 1944.266 kb on - strand, within mutYat 1944.344 kb on + strand, within mutYat 1944.460 kb on + strandat 1944.461 kb on - strandat 1944.617 kb on + strandat 1944.618 kb on - strandat 1944.618 kb on - strandat 1944.618 kb on - strandat 1944.661 kb on + strandat 1944.733 kb on - strandat 1944.790 kb on + strand, within trmBat 1944.825 kb on + strand, within trmBat 1944.826 kb on - strand, within trmBat 1944.826 kb on - strand, within trmBat 1944.826 kb on - strand, within trmBat 1945.004 kb on + strand, within trmBat 1945.050 kb on + strand, within trmBat 1945.051 kb on - strand, within trmBat 1945.123 kb on - strand, within trmBat 1945.163 kb on + strand, within trmBat 1945.220 kb on - strand, within trmBat 1945.230 kb on + strand, within trmBat 1945.230 kb on + strand, within trmBat 1945.230 kb on + strand, within trmBat 1945.231 kb on - strand, within trmBat 1945.231 kb on - strand, within trmBat 1945.263 kb on + strand, within trmBat 1945.288 kb on + strand, within trmBat 1945.367 kb on + strandat 1945.367 kb on + strandat 1945.368 kb on - strandat 1945.368 kb on - strandat 1945.387 kb on + strandat 1945.388 kb on - strandat 1945.388 kb on - strandat 1945.505 kb on + strand, within yggLat 1945.505 kb on + strand, within yggLat 1945.523 kb on + strand, within yggLat 1945.523 kb on + strand, within yggLat 1945.524 kb on - strand, within yggLat 1945.524 kb on - strand, within yggLat 1945.647 kb on - strand, within yggLat 1945.678 kb on + strand, within yggLat 1945.714 kb on - strandat 1945.723 kb on + strandat 1945.766 kb on - strandat 1945.812 kb on + strandat 1945.813 kb on - strandat 1945.898 kb on + strandat 1945.898 kb on + strandat 1945.898 kb on + strandat 1945.898 kb on + strandat 1945.898 kb on + strandat 1945.899 kb on - strandat 1945.899 kb on - strandat 1946.070 kb on + strand, within yggNat 1946.070 kb on + strand, within yggNat 1946.070 kb on + strand, within yggNat 1946.071 kb on - strand, within yggNat 1946.086 kb on - strand, within yggNat 1946.124 kb on + strand, within yggNat 1946.137 kb on + strand, within yggNat 1946.138 kb on - strand, within yggNat 1946.138 kb on - strand, within yggNat 1946.141 kb on + strand, within yggNat 1946.141 kb on + strand, within yggNat 1946.142 kb on - strand, within yggNat 1946.195 kb on + strand, within yggNat 1946.213 kb on + strand, within yggNat 1946.334 kb on + strand, within yggNat 1946.334 kb on + strand, within yggNat 1946.336 kb on + strand, within yggNat 1946.337 kb on - strand, within yggN

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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1,943,736 + mutY BNILDI_09645 0.26 -1.8
1,943,737 - mutY BNILDI_09645 0.27 -0.4
1,943,737 - mutY BNILDI_09645 0.27 -0.7
1,943,778 + mutY BNILDI_09645 0.30 +2.5
1,943,778 + mutY BNILDI_09645 0.30 -0.7
1,943,778 + mutY BNILDI_09645 0.30 +0.9
1,943,779 - mutY BNILDI_09645 0.31 +0.5
1,943,885 + mutY BNILDI_09645 0.41 +0.2
1,943,885 + mutY BNILDI_09645 0.41 +0.2
1,943,886 - mutY BNILDI_09645 0.41 -0.4
1,943,970 - mutY BNILDI_09645 0.49 +1.2
1,944,018 + mutY BNILDI_09645 0.53 +0.7
1,944,064 + mutY BNILDI_09645 0.58 -0.6
1,944,065 - mutY BNILDI_09645 0.58 +0.7
1,944,213 + mutY BNILDI_09645 0.72 +0.6
1,944,263 - mutY BNILDI_09645 0.77 -0.5
1,944,266 - mutY BNILDI_09645 0.77 +0.1
1,944,344 + mutY BNILDI_09645 0.84 +0.1
1,944,460 + +2.2
1,944,461 - +0.4
1,944,617 + +0.3
1,944,618 - -0.9
1,944,618 - +0.7
1,944,618 - +0.2
1,944,661 + +1.1
1,944,733 - +0.4
1,944,790 + trmB BNILDI_09650 0.17 +0.3
1,944,825 + trmB BNILDI_09650 0.22 +0.7
1,944,826 - trmB BNILDI_09650 0.22 -0.4
1,944,826 - trmB BNILDI_09650 0.22 +0.2
1,944,826 - trmB BNILDI_09650 0.22 +1.2
1,945,004 + trmB BNILDI_09650 0.46 -0.5
1,945,050 + trmB BNILDI_09650 0.53 -0.3
1,945,051 - trmB BNILDI_09650 0.53 +0.2
1,945,123 - trmB BNILDI_09650 0.63 -0.6
1,945,163 + trmB BNILDI_09650 0.68 +0.0
1,945,220 - trmB BNILDI_09650 0.76 +0.8
1,945,230 + trmB BNILDI_09650 0.78 -0.7
1,945,230 + trmB BNILDI_09650 0.78 -0.1
1,945,230 + trmB BNILDI_09650 0.78 +1.3
1,945,231 - trmB BNILDI_09650 0.78 +1.0
1,945,231 - trmB BNILDI_09650 0.78 +0.7
1,945,263 + trmB BNILDI_09650 0.82 -1.3
1,945,288 + trmB BNILDI_09650 0.86 -0.1
1,945,367 + +0.0
1,945,367 + -1.0
1,945,368 - +1.7
1,945,368 - -0.5
1,945,387 + +2.1
1,945,388 - +1.3
1,945,388 - +0.4
1,945,505 + yggL BNILDI_09655 0.35 +0.9
1,945,505 + yggL BNILDI_09655 0.35 -0.9
1,945,523 + yggL BNILDI_09655 0.41 -0.2
1,945,523 + yggL BNILDI_09655 0.41 -0.1
1,945,524 - yggL BNILDI_09655 0.41 -0.4
1,945,524 - yggL BNILDI_09655 0.41 -1.1
1,945,647 - yggL BNILDI_09655 0.79 +0.4
1,945,678 + yggL BNILDI_09655 0.88 +0.0
1,945,714 - -0.9
1,945,723 + -0.2
1,945,766 - +0.2
1,945,812 + +0.9
1,945,813 - -0.5
1,945,898 + +0.3
1,945,898 + -0.1
1,945,898 + -0.3
1,945,898 + -0.3
1,945,898 + +0.2
1,945,899 - -0.4
1,945,899 - -0.0
1,946,070 + yggN BNILDI_09660 0.24 -0.4
1,946,070 + yggN BNILDI_09660 0.24 -0.4
1,946,070 + yggN BNILDI_09660 0.24 -3.2
1,946,071 - yggN BNILDI_09660 0.24 +1.7
1,946,086 - yggN BNILDI_09660 0.26 -0.7
1,946,124 + yggN BNILDI_09660 0.31 -0.3
1,946,137 + yggN BNILDI_09660 0.33 +0.3
1,946,138 - yggN BNILDI_09660 0.33 +1.7
1,946,138 - yggN BNILDI_09660 0.33 -0.1
1,946,141 + yggN BNILDI_09660 0.34 -0.8
1,946,141 + yggN BNILDI_09660 0.34 -0.1
1,946,142 - yggN BNILDI_09660 0.34 -0.3
1,946,195 + yggN BNILDI_09660 0.41 +0.1
1,946,213 + yggN BNILDI_09660 0.44 +0.4
1,946,334 + yggN BNILDI_09660 0.60 -1.3
1,946,334 + yggN BNILDI_09660 0.60 +0.6
1,946,336 + yggN BNILDI_09660 0.61 +0.2
1,946,337 - yggN BNILDI_09660 0.61 +1.3

Or see this region's nucleotide sequence