Strain Fitness in Escherichia coli ECRC62 around BNILDI_04870

Experiment: JK38

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwzyE and rffM are separated by 2 nucleotidesrffM and yifK are separated by 190 nucleotidesyifK and Arg_trna are separated by 102 nucleotidesArg_trna and His_trna are separated by 58 nucleotidesHis_trna and Leu_trna are separated by 20 nucleotidesLeu_trna and Pro_trna are separated by 42 nucleotidesPro_trna and aslB are separated by 146 nucleotides BNILDI_04860: wzyE - ECA oligosaccharide polymerase, at 965,526 to 966,878 wzyE BNILDI_04865: rffM - lipopolysaccharide N-acetylmannosaminouronosyltransferase, at 966,881 to 967,621 rffM BNILDI_04870: yifK - amino acid permease, at 967,812 to 969,197 yifK BNILDI_04875: Arg_trna - tRNA-Arg(ccg), at 969,300 to 969,376 _trna BNILDI_04880: His_trna - tRNA-His(gtg), at 969,435 to 969,510 _trna BNILDI_04885: Leu_trna - tRNA-Leu(cag), at 969,531 to 969,617 _trna BNILDI_04890: Pro_trna - tRNA-Pro(tgg), at 969,660 to 969,736 _trna BNILDI_04895: aslB - anaerobic sulfatase maturase, at 969,883 to 971,118 aslB Position (kb) 967 968 969 970Strain fitness (log2 ratio) -2 -1 0 1 2at 966.843 kb on - strandat 966.887 kb on - strandat 966.909 kb on - strandat 966.909 kb on - strandat 966.910 kb on + strandat 966.924 kb on + strandat 966.989 kb on - strand, within rffMat 966.989 kb on - strand, within rffMat 967.042 kb on + strand, within rffMat 967.042 kb on + strand, within rffMat 967.042 kb on + strand, within rffMat 967.043 kb on - strand, within rffMat 967.062 kb on + strand, within rffMat 967.063 kb on - strand, within rffMat 967.063 kb on - strand, within rffMat 967.082 kb on - strand, within rffMat 967.085 kb on + strand, within rffMat 967.107 kb on + strand, within rffMat 967.108 kb on - strand, within rffMat 967.259 kb on - strand, within rffMat 967.445 kb on + strand, within rffMat 967.445 kb on + strand, within rffMat 967.445 kb on + strand, within rffMat 967.445 kb on + strand, within rffMat 967.446 kb on - strand, within rffMat 967.446 kb on - strand, within rffMat 967.446 kb on - strand, within rffMat 967.447 kb on + strand, within rffMat 967.448 kb on - strand, within rffMat 967.466 kb on + strand, within rffMat 967.467 kb on - strand, within rffMat 967.467 kb on - strand, within rffMat 967.467 kb on - strand, within rffMat 967.489 kb on - strand, within rffMat 967.536 kb on - strand, within rffMat 967.589 kb on + strandat 967.593 kb on + strandat 967.681 kb on - strandat 967.731 kb on - strandat 967.756 kb on + strandat 967.811 kb on + strandat 967.821 kb on - strandat 967.835 kb on - strandat 967.945 kb on + strandat 967.956 kb on + strand, within yifKat 967.958 kb on + strand, within yifKat 968.058 kb on + strand, within yifKat 968.058 kb on + strand, within yifKat 968.060 kb on + strand, within yifKat 968.060 kb on + strand, within yifKat 968.061 kb on - strand, within yifKat 968.061 kb on - strand, within yifKat 968.152 kb on - strand, within yifKat 968.248 kb on - strand, within yifKat 968.367 kb on - strand, within yifKat 968.558 kb on - strand, within yifKat 968.651 kb on + strand, within yifKat 968.721 kb on + strand, within yifKat 968.721 kb on + strand, within yifKat 968.721 kb on + strand, within yifKat 968.722 kb on - strand, within yifKat 968.722 kb on - strand, within yifKat 968.722 kb on - strand, within yifKat 968.735 kb on + strand, within yifKat 968.735 kb on + strand, within yifKat 968.736 kb on - strand, within yifKat 968.736 kb on - strand, within yifKat 968.828 kb on + strand, within yifKat 968.828 kb on + strand, within yifKat 968.828 kb on + strand, within yifKat 968.829 kb on - strand, within yifKat 968.829 kb on - strand, within yifKat 968.829 kb on - strand, within yifKat 968.829 kb on - strand, within yifKat 968.835 kb on - strand, within yifKat 968.842 kb on + strand, within yifKat 968.842 kb on + strand, within yifKat 968.862 kb on + strand, within yifKat 968.862 kb on + strand, within yifKat 968.862 kb on + strand, within yifKat 968.862 kb on + strand, within yifKat 968.863 kb on - strand, within yifKat 968.863 kb on - strand, within yifKat 968.863 kb on - strand, within yifKat 968.896 kb on - strand, within yifKat 968.901 kb on + strand, within yifKat 968.902 kb on - strand, within yifKat 968.979 kb on - strand, within yifKat 969.033 kb on + strand, within yifKat 969.033 kb on + strand, within yifKat 969.033 kb on + strand, within yifKat 969.033 kb on + strand, within yifKat 969.034 kb on - strand, within yifKat 969.034 kb on - strand, within yifKat 969.034 kb on - strand, within yifKat 969.034 kb on - strand, within yifKat 969.034 kb on - strand, within yifKat 969.038 kb on - strand, within yifKat 969.075 kb on + strandat 969.076 kb on - strandat 969.095 kb on + strandat 969.096 kb on - strandat 969.162 kb on - strandat 969.183 kb on - strandat 969.408 kb on + strandat 969.811 kb on + strandat 969.946 kb on - strandat 969.962 kb on - strandat 970.039 kb on + strand, within aslBat 970.039 kb on + strand, within aslBat 970.052 kb on - strand, within aslBat 970.162 kb on + strand, within aslBat 970.181 kb on + strand, within aslBat 970.182 kb on - strand, within aslBat 970.182 kb on - strand, within aslBat 970.182 kb on - strand, within aslBat 970.185 kb on + strand, within aslBat 970.186 kb on - strand, within aslBat 970.186 kb on - strand, within aslB

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Per-strain Table

Position Strand Gene LocusTag Fraction JK38
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966,843 - +0.3
966,887 - +0.3
966,909 - -0.4
966,909 - -0.6
966,910 + -0.2
966,924 + -1.3
966,989 - rffM BNILDI_04865 0.15 -0.2
966,989 - rffM BNILDI_04865 0.15 -0.3
967,042 + rffM BNILDI_04865 0.22 +1.9
967,042 + rffM BNILDI_04865 0.22 -0.3
967,042 + rffM BNILDI_04865 0.22 -0.2
967,043 - rffM BNILDI_04865 0.22 +0.6
967,062 + rffM BNILDI_04865 0.24 +0.4
967,063 - rffM BNILDI_04865 0.25 -0.5
967,063 - rffM BNILDI_04865 0.25 +0.1
967,082 - rffM BNILDI_04865 0.27 +0.3
967,085 + rffM BNILDI_04865 0.28 -0.2
967,107 + rffM BNILDI_04865 0.30 +0.9
967,108 - rffM BNILDI_04865 0.31 -1.2
967,259 - rffM BNILDI_04865 0.51 +0.3
967,445 + rffM BNILDI_04865 0.76 +0.5
967,445 + rffM BNILDI_04865 0.76 -0.1
967,445 + rffM BNILDI_04865 0.76 +0.1
967,445 + rffM BNILDI_04865 0.76 -0.9
967,446 - rffM BNILDI_04865 0.76 -0.8
967,446 - rffM BNILDI_04865 0.76 -1.6
967,446 - rffM BNILDI_04865 0.76 -0.4
967,447 + rffM BNILDI_04865 0.76 -0.2
967,448 - rffM BNILDI_04865 0.77 +0.1
967,466 + rffM BNILDI_04865 0.79 -0.1
967,467 - rffM BNILDI_04865 0.79 +1.6
967,467 - rffM BNILDI_04865 0.79 +0.9
967,467 - rffM BNILDI_04865 0.79 +2.7
967,489 - rffM BNILDI_04865 0.82 -1.3
967,536 - rffM BNILDI_04865 0.88 -0.2
967,589 + +0.1
967,593 + +0.1
967,681 - +0.0
967,731 - -0.4
967,756 + +0.3
967,811 + -0.1
967,821 - -0.6
967,835 - -0.2
967,945 + +0.6
967,956 + yifK BNILDI_04870 0.10 +0.1
967,958 + yifK BNILDI_04870 0.11 +0.3
968,058 + yifK BNILDI_04870 0.18 +0.5
968,058 + yifK BNILDI_04870 0.18 -0.1
968,060 + yifK BNILDI_04870 0.18 +2.2
968,060 + yifK BNILDI_04870 0.18 -0.9
968,061 - yifK BNILDI_04870 0.18 -0.6
968,061 - yifK BNILDI_04870 0.18 -0.5
968,152 - yifK BNILDI_04870 0.25 +0.6
968,248 - yifK BNILDI_04870 0.31 +1.3
968,367 - yifK BNILDI_04870 0.40 +1.2
968,558 - yifK BNILDI_04870 0.54 -0.3
968,651 + yifK BNILDI_04870 0.61 +0.1
968,721 + yifK BNILDI_04870 0.66 -0.7
968,721 + yifK BNILDI_04870 0.66 +0.6
968,721 + yifK BNILDI_04870 0.66 +1.9
968,722 - yifK BNILDI_04870 0.66 +0.9
968,722 - yifK BNILDI_04870 0.66 -1.4
968,722 - yifK BNILDI_04870 0.66 -0.5
968,735 + yifK BNILDI_04870 0.67 -0.6
968,735 + yifK BNILDI_04870 0.67 -1.0
968,736 - yifK BNILDI_04870 0.67 -0.9
968,736 - yifK BNILDI_04870 0.67 -0.8
968,828 + yifK BNILDI_04870 0.73 +0.2
968,828 + yifK BNILDI_04870 0.73 -0.1
968,828 + yifK BNILDI_04870 0.73 -0.0
968,829 - yifK BNILDI_04870 0.73 +0.0
968,829 - yifK BNILDI_04870 0.73 -0.8
968,829 - yifK BNILDI_04870 0.73 +0.0
968,829 - yifK BNILDI_04870 0.73 -0.0
968,835 - yifK BNILDI_04870 0.74 +0.3
968,842 + yifK BNILDI_04870 0.74 -1.6
968,842 + yifK BNILDI_04870 0.74 +1.3
968,862 + yifK BNILDI_04870 0.76 +0.6
968,862 + yifK BNILDI_04870 0.76 +1.2
968,862 + yifK BNILDI_04870 0.76 +0.8
968,862 + yifK BNILDI_04870 0.76 +1.1
968,863 - yifK BNILDI_04870 0.76 +0.5
968,863 - yifK BNILDI_04870 0.76 -0.1
968,863 - yifK BNILDI_04870 0.76 -0.8
968,896 - yifK BNILDI_04870 0.78 +1.3
968,901 + yifK BNILDI_04870 0.79 -0.4
968,902 - yifK BNILDI_04870 0.79 +0.3
968,979 - yifK BNILDI_04870 0.84 -0.1
969,033 + yifK BNILDI_04870 0.88 +0.8
969,033 + yifK BNILDI_04870 0.88 +0.3
969,033 + yifK BNILDI_04870 0.88 -0.3
969,033 + yifK BNILDI_04870 0.88 +0.1
969,034 - yifK BNILDI_04870 0.88 -0.2
969,034 - yifK BNILDI_04870 0.88 +0.1
969,034 - yifK BNILDI_04870 0.88 +0.9
969,034 - yifK BNILDI_04870 0.88 +1.4
969,034 - yifK BNILDI_04870 0.88 +0.9
969,038 - yifK BNILDI_04870 0.88 -0.0
969,075 + +0.2
969,076 - -0.1
969,095 + +1.9
969,096 - -0.0
969,162 - -0.0
969,183 - +0.3
969,408 + -0.1
969,811 + +0.7
969,946 - +1.3
969,962 - -0.8
970,039 + aslB BNILDI_04895 0.13 -0.1
970,039 + aslB BNILDI_04895 0.13 -0.7
970,052 - aslB BNILDI_04895 0.14 +0.5
970,162 + aslB BNILDI_04895 0.23 -1.0
970,181 + aslB BNILDI_04895 0.24 -1.0
970,182 - aslB BNILDI_04895 0.24 +0.3
970,182 - aslB BNILDI_04895 0.24 -2.1
970,182 - aslB BNILDI_04895 0.24 +0.9
970,185 + aslB BNILDI_04895 0.24 +0.3
970,186 - aslB BNILDI_04895 0.25 -0.1
970,186 - aslB BNILDI_04895 0.25 +0.6

Or see this region's nucleotide sequence