Experiment: JK38
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rffT and wzyE overlap by 4 nucleotides wzyE and rffM are separated by 2 nucleotides rffM and yifK are separated by 190 nucleotides
BNILDI_04855: rffT - TDP-N-acetylfucosamine:lipid II N-acetylfucosaminyltransferase, at 964,450 to 965,529
rffT
BNILDI_04860: wzyE - ECA oligosaccharide polymerase, at 965,526 to 966,878
wzyE
BNILDI_04865: rffM - lipopolysaccharide N-acetylmannosaminouronosyltransferase, at 966,881 to 967,621
rffM
BNILDI_04870: yifK - amino acid permease, at 967,812 to 969,197
yifK
Position (kb)
965
966
967 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1
2
3 at 964.587 kb on - strand, within rffT at 964.594 kb on - strand, within rffT at 964.594 kb on - strand, within rffT at 964.747 kb on - strand, within rffT at 964.861 kb on + strand, within rffT at 964.865 kb on + strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 964.866 kb on - strand, within rffT at 965.054 kb on + strand, within rffT at 965.131 kb on + strand, within rffT at 965.131 kb on + strand, within rffT at 965.131 kb on + strand, within rffT at 965.132 kb on - strand, within rffT at 965.132 kb on - strand, within rffT at 965.256 kb on + strand, within rffT at 965.257 kb on - strand, within rffT at 965.406 kb on + strand, within rffT at 965.473 kb on - strand at 965.476 kb on + strand at 965.476 kb on + strand at 965.477 kb on - strand at 965.480 kb on + strand at 965.480 kb on + strand at 965.481 kb on - strand at 965.778 kb on + strand, within wzyE at 965.781 kb on + strand, within wzyE at 965.781 kb on + strand, within wzyE at 965.782 kb on - strand, within wzyE at 965.825 kb on - strand, within wzyE at 965.870 kb on + strand, within wzyE at 965.958 kb on + strand, within wzyE at 965.998 kb on + strand, within wzyE at 966.013 kb on - strand, within wzyE at 966.013 kb on - strand, within wzyE at 966.114 kb on + strand, within wzyE at 966.114 kb on + strand, within wzyE at 966.115 kb on - strand, within wzyE at 966.115 kb on - strand, within wzyE at 966.116 kb on + strand, within wzyE at 966.116 kb on + strand, within wzyE at 966.116 kb on + strand, within wzyE at 966.116 kb on + strand, within wzyE at 966.117 kb on - strand, within wzyE at 966.300 kb on + strand, within wzyE at 966.301 kb on - strand, within wzyE at 966.301 kb on - strand, within wzyE at 966.302 kb on + strand, within wzyE at 966.303 kb on - strand, within wzyE at 966.303 kb on - strand, within wzyE at 966.303 kb on - strand, within wzyE at 966.369 kb on + strand, within wzyE at 966.370 kb on - strand, within wzyE at 966.481 kb on - strand, within wzyE at 966.481 kb on - strand, within wzyE at 966.539 kb on - strand, within wzyE at 966.539 kb on - strand, within wzyE at 966.539 kb on - strand, within wzyE at 966.628 kb on - strand, within wzyE at 966.660 kb on - strand, within wzyE at 966.705 kb on - strand, within wzyE at 966.843 kb on - strand at 966.887 kb on - strand at 966.909 kb on - strand at 966.909 kb on - strand at 966.910 kb on + strand at 966.924 kb on + strand at 966.989 kb on - strand, within rffM at 966.989 kb on - strand, within rffM at 967.042 kb on + strand, within rffM at 967.042 kb on + strand, within rffM at 967.042 kb on + strand, within rffM at 967.043 kb on - strand, within rffM at 967.062 kb on + strand, within rffM at 967.063 kb on - strand, within rffM at 967.063 kb on - strand, within rffM at 967.082 kb on - strand, within rffM at 967.085 kb on + strand, within rffM at 967.107 kb on + strand, within rffM at 967.108 kb on - strand, within rffM at 967.259 kb on - strand, within rffM at 967.445 kb on + strand, within rffM at 967.445 kb on + strand, within rffM at 967.445 kb on + strand, within rffM at 967.445 kb on + strand, within rffM at 967.446 kb on - strand, within rffM at 967.446 kb on - strand, within rffM at 967.446 kb on - strand, within rffM at 967.447 kb on + strand, within rffM at 967.448 kb on - strand, within rffM at 967.466 kb on + strand, within rffM at 967.467 kb on - strand, within rffM at 967.467 kb on - strand, within rffM at 967.467 kb on - strand, within rffM at 967.489 kb on - strand, within rffM at 967.536 kb on - strand, within rffM at 967.589 kb on + strand at 967.593 kb on + strand at 967.681 kb on - strand at 967.731 kb on - strand at 967.756 kb on + strand at 967.811 kb on + strand at 967.821 kb on - strand at 967.835 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction JK38 remove 964,587 - rffT BNILDI_04855 0.13 +1.9 964,594 - rffT BNILDI_04855 0.13 -0.2 964,594 - rffT BNILDI_04855 0.13 +0.1 964,747 - rffT BNILDI_04855 0.28 +0.4 964,861 + rffT BNILDI_04855 0.38 +0.3 964,865 + rffT BNILDI_04855 0.38 -0.2 964,866 - rffT BNILDI_04855 0.39 +1.9 964,866 - rffT BNILDI_04855 0.39 -0.7 964,866 - rffT BNILDI_04855 0.39 -0.7 964,866 - rffT BNILDI_04855 0.39 +0.7 964,866 - rffT BNILDI_04855 0.39 -0.7 964,866 - rffT BNILDI_04855 0.39 +1.2 964,866 - rffT BNILDI_04855 0.39 -0.4 965,054 + rffT BNILDI_04855 0.56 +0.9 965,131 + rffT BNILDI_04855 0.63 -0.1 965,131 + rffT BNILDI_04855 0.63 +1.1 965,131 + rffT BNILDI_04855 0.63 +0.3 965,132 - rffT BNILDI_04855 0.63 +0.0 965,132 - rffT BNILDI_04855 0.63 +0.3 965,256 + rffT BNILDI_04855 0.75 -0.4 965,257 - rffT BNILDI_04855 0.75 -0.1 965,406 + rffT BNILDI_04855 0.89 -0.2 965,473 - -0.4 965,476 + +0.2 965,476 + +0.1 965,477 - -1.5 965,480 + +1.4 965,480 + -0.1 965,481 - -1.5 965,778 + wzyE BNILDI_04860 0.19 +0.3 965,781 + wzyE BNILDI_04860 0.19 -1.4 965,781 + wzyE BNILDI_04860 0.19 -4.5 965,782 - wzyE BNILDI_04860 0.19 -2.0 965,825 - wzyE BNILDI_04860 0.22 -0.9 965,870 + wzyE BNILDI_04860 0.25 -5.8 965,958 + wzyE BNILDI_04860 0.32 -6.6 965,998 + wzyE BNILDI_04860 0.35 -1.5 966,013 - wzyE BNILDI_04860 0.36 -1.7 966,013 - wzyE BNILDI_04860 0.36 -2.9 966,114 + wzyE BNILDI_04860 0.43 -2.8 966,114 + wzyE BNILDI_04860 0.43 -2.0 966,115 - wzyE BNILDI_04860 0.44 -1.4 966,115 - wzyE BNILDI_04860 0.44 -1.9 966,116 + wzyE BNILDI_04860 0.44 -2.8 966,116 + wzyE BNILDI_04860 0.44 -0.1 966,116 + wzyE BNILDI_04860 0.44 -6.1 966,116 + wzyE BNILDI_04860 0.44 -0.4 966,117 - wzyE BNILDI_04860 0.44 -4.5 966,300 + wzyE BNILDI_04860 0.57 -1.4 966,301 - wzyE BNILDI_04860 0.57 -3.4 966,301 - wzyE BNILDI_04860 0.57 -2.1 966,302 + wzyE BNILDI_04860 0.57 -0.4 966,303 - wzyE BNILDI_04860 0.57 -2.9 966,303 - wzyE BNILDI_04860 0.57 -6.2 966,303 - wzyE BNILDI_04860 0.57 -3.6 966,369 + wzyE BNILDI_04860 0.62 +0.9 966,370 - wzyE BNILDI_04860 0.62 -1.3 966,481 - wzyE BNILDI_04860 0.71 -2.7 966,481 - wzyE BNILDI_04860 0.71 -1.1 966,539 - wzyE BNILDI_04860 0.75 +0.3 966,539 - wzyE BNILDI_04860 0.75 -1.1 966,539 - wzyE BNILDI_04860 0.75 -6.3 966,628 - wzyE BNILDI_04860 0.81 -3.4 966,660 - wzyE BNILDI_04860 0.84 -1.8 966,705 - wzyE BNILDI_04860 0.87 -6.0 966,843 - +0.3 966,887 - +0.3 966,909 - -0.4 966,909 - -0.6 966,910 + -0.2 966,924 + -1.3 966,989 - rffM BNILDI_04865 0.15 -0.2 966,989 - rffM BNILDI_04865 0.15 -0.3 967,042 + rffM BNILDI_04865 0.22 +1.9 967,042 + rffM BNILDI_04865 0.22 -0.3 967,042 + rffM BNILDI_04865 0.22 -0.2 967,043 - rffM BNILDI_04865 0.22 +0.6 967,062 + rffM BNILDI_04865 0.24 +0.4 967,063 - rffM BNILDI_04865 0.25 -0.5 967,063 - rffM BNILDI_04865 0.25 +0.1 967,082 - rffM BNILDI_04865 0.27 +0.3 967,085 + rffM BNILDI_04865 0.28 -0.2 967,107 + rffM BNILDI_04865 0.30 +0.9 967,108 - rffM BNILDI_04865 0.31 -1.2 967,259 - rffM BNILDI_04865 0.51 +0.3 967,445 + rffM BNILDI_04865 0.76 +0.6 967,445 + rffM BNILDI_04865 0.76 -0.1 967,445 + rffM BNILDI_04865 0.76 +0.1 967,445 + rffM BNILDI_04865 0.76 -0.9 967,446 - rffM BNILDI_04865 0.76 -0.8 967,446 - rffM BNILDI_04865 0.76 -1.5 967,446 - rffM BNILDI_04865 0.76 -0.4 967,447 + rffM BNILDI_04865 0.76 -0.2 967,448 - rffM BNILDI_04865 0.77 +0.1 967,466 + rffM BNILDI_04865 0.79 -0.1 967,467 - rffM BNILDI_04865 0.79 +1.6 967,467 - rffM BNILDI_04865 0.79 +0.9 967,467 - rffM BNILDI_04865 0.79 +2.8 967,489 - rffM BNILDI_04865 0.82 -1.3 967,536 - rffM BNILDI_04865 0.88 -0.2 967,589 + +0.1 967,593 + +0.1 967,681 - +0.1 967,731 - -0.4 967,756 + +0.3 967,811 + -0.1 967,821 - -0.6 967,835 - -0.1
Or see this region's nucleotide sequence