Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF719 and GFF720 are separated by 15 nucleotides GFF720 and GFF721 overlap by 4 nucleotides GFF721 and GFF722 overlap by 4 nucleotides
GFF719 - no description, at 777,596 to 778,591
GFF719
GFF720 - no description, at 778,607 to 778,771
GFF720
GFF721 - O-acetyl-ADP-ribose deacetylase, at 778,768 to 779,274
GFF721
GFF722 - hypothetical protein, at 779,271 to 780,047
GFF722
Position (kb)
778
779 Strain fitness (log2 ratio)
-2
-1
0
1 at 777.630 kb on + strand at 777.631 kb on - strand at 777.883 kb on - strand, within GFF719 at 777.883 kb on - strand, within GFF719 at 777.906 kb on + strand, within GFF719 at 777.907 kb on - strand, within GFF719 at 777.907 kb on - strand, within GFF719 at 777.907 kb on - strand, within GFF719 at 777.907 kb on - strand, within GFF719 at 777.907 kb on - strand, within GFF719 at 777.922 kb on - strand, within GFF719 at 777.993 kb on + strand, within GFF719 at 777.994 kb on - strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.074 kb on + strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.075 kb on - strand, within GFF719 at 778.143 kb on + strand, within GFF719 at 778.143 kb on + strand, within GFF719 at 778.146 kb on + strand, within GFF719 at 778.146 kb on + strand, within GFF719 at 778.146 kb on + strand, within GFF719 at 778.146 kb on + strand, within GFF719 at 778.146 kb on + strand, within GFF719 at 778.147 kb on - strand, within GFF719 at 778.147 kb on - strand, within GFF719 at 778.147 kb on - strand, within GFF719 at 778.224 kb on + strand, within GFF719 at 778.225 kb on - strand, within GFF719 at 778.225 kb on - strand, within GFF719 at 778.225 kb on - strand, within GFF719 at 778.225 kb on - strand, within GFF719 at 778.263 kb on + strand, within GFF719 at 778.263 kb on + strand, within GFF719 at 778.264 kb on - strand, within GFF719 at 778.264 kb on - strand, within GFF719 at 778.288 kb on - strand, within GFF719 at 778.317 kb on + strand, within GFF719 at 778.318 kb on - strand, within GFF719 at 778.479 kb on + strand, within GFF719 at 778.479 kb on + strand, within GFF719 at 778.480 kb on - strand, within GFF719 at 778.480 kb on - strand, within GFF719 at 778.480 kb on - strand, within GFF719 at 778.668 kb on + strand, within GFF720 at 778.668 kb on + strand, within GFF720 at 778.668 kb on + strand, within GFF720 at 778.668 kb on + strand, within GFF720 at 778.735 kb on + strand, within GFF720 at 778.735 kb on + strand, within GFF720 at 778.735 kb on + strand, within GFF720 at 778.736 kb on - strand, within GFF720 at 778.995 kb on + strand, within GFF721 at 778.995 kb on + strand, within GFF721 at 778.996 kb on - strand, within GFF721 at 778.996 kb on - strand, within GFF721 at 778.996 kb on - strand, within GFF721 at 778.996 kb on - strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.131 kb on + strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.132 kb on - strand, within GFF721 at 779.177 kb on + strand, within GFF721 at 779.178 kb on - strand, within GFF721 at 779.255 kb on - strand at 779.286 kb on + strand at 779.287 kb on - strand at 779.287 kb on - strand at 779.319 kb on + strand at 779.730 kb on + strand, within GFF722 at 779.731 kb on - strand, within GFF722 at 779.731 kb on - strand, within GFF722 at 779.731 kb on - strand, within GFF722
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 777,630 + -2.0 777,631 - +1.2 777,883 - GFF719 0.29 +0.2 777,883 - GFF719 0.29 -0.4 777,906 + GFF719 0.31 -1.4 777,907 - GFF719 0.31 -0.5 777,907 - GFF719 0.31 -0.4 777,907 - GFF719 0.31 -0.2 777,907 - GFF719 0.31 -1.1 777,907 - GFF719 0.31 -1.0 777,922 - GFF719 0.33 -0.5 777,993 + GFF719 0.40 +0.2 777,994 - GFF719 0.40 +0.3 778,074 + GFF719 0.48 -0.5 778,074 + GFF719 0.48 +0.0 778,074 + GFF719 0.48 +0.4 778,074 + GFF719 0.48 +0.2 778,074 + GFF719 0.48 +0.3 778,074 + GFF719 0.48 +0.2 778,074 + GFF719 0.48 +0.2 778,074 + GFF719 0.48 -0.6 778,075 - GFF719 0.48 -0.1 778,075 - GFF719 0.48 -0.3 778,075 - GFF719 0.48 +0.1 778,075 - GFF719 0.48 -2.0 778,075 - GFF719 0.48 +0.6 778,075 - GFF719 0.48 -1.0 778,075 - GFF719 0.48 -2.5 778,075 - GFF719 0.48 +0.6 778,075 - GFF719 0.48 -0.5 778,075 - GFF719 0.48 +0.3 778,143 + GFF719 0.55 -0.4 778,143 + GFF719 0.55 +0.6 778,146 + GFF719 0.55 -1.0 778,146 + GFF719 0.55 +1.5 778,146 + GFF719 0.55 +0.8 778,146 + GFF719 0.55 -1.6 778,146 + GFF719 0.55 +0.4 778,147 - GFF719 0.55 -0.5 778,147 - GFF719 0.55 +0.4 778,147 - GFF719 0.55 -0.8 778,224 + GFF719 0.63 -0.0 778,225 - GFF719 0.63 -1.1 778,225 - GFF719 0.63 -1.1 778,225 - GFF719 0.63 -0.4 778,225 - GFF719 0.63 -1.1 778,263 + GFF719 0.67 -0.8 778,263 + GFF719 0.67 -0.7 778,264 - GFF719 0.67 -1.2 778,264 - GFF719 0.67 -0.8 778,288 - GFF719 0.69 +0.2 778,317 + GFF719 0.72 -1.6 778,318 - GFF719 0.72 -0.4 778,479 + GFF719 0.89 -0.7 778,479 + GFF719 0.89 -0.4 778,480 - GFF719 0.89 -1.7 778,480 - GFF719 0.89 +0.7 778,480 - GFF719 0.89 -1.1 778,668 + GFF720 0.37 -0.9 778,668 + GFF720 0.37 -0.9 778,668 + GFF720 0.37 -0.8 778,668 + GFF720 0.37 -0.8 778,735 + GFF720 0.78 -0.7 778,735 + GFF720 0.78 +0.6 778,735 + GFF720 0.78 -0.4 778,736 - GFF720 0.78 -1.4 778,995 + GFF721 0.45 -0.8 778,995 + GFF721 0.45 +0.0 778,996 - GFF721 0.45 -0.6 778,996 - GFF721 0.45 -1.1 778,996 - GFF721 0.45 -0.8 778,996 - GFF721 0.45 -0.0 779,131 + GFF721 0.72 -1.8 779,131 + GFF721 0.72 +1.0 779,131 + GFF721 0.72 -0.6 779,131 + GFF721 0.72 -0.3 779,131 + GFF721 0.72 -2.0 779,131 + GFF721 0.72 +0.5 779,131 + GFF721 0.72 +0.4 779,131 + GFF721 0.72 -0.1 779,132 - GFF721 0.72 +0.2 779,132 - GFF721 0.72 +0.2 779,132 - GFF721 0.72 -0.7 779,132 - GFF721 0.72 +0.2 779,132 - GFF721 0.72 -1.0 779,132 - GFF721 0.72 -0.5 779,132 - GFF721 0.72 -0.3 779,132 - GFF721 0.72 +0.5 779,132 - GFF721 0.72 +0.2 779,132 - GFF721 0.72 +0.3 779,132 - GFF721 0.72 -0.5 779,132 - GFF721 0.72 -1.0 779,177 + GFF721 0.81 +0.4 779,178 - GFF721 0.81 +0.1 779,255 - +0.3 779,286 + +0.5 779,287 - -0.3 779,287 - -0.5 779,319 + -0.7 779,730 + GFF722 0.59 -2.4 779,731 - GFF722 0.59 -1.6 779,731 - GFF722 0.59 +0.4 779,731 - GFF722 0.59 +0.2
Or see this region's nucleotide sequence