Strain Fitness in Variovorax sp. SCN45 around GFF7033

Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF7033 and GFF7034 overlap by 4 nucleotidesGFF7034 and GFF7035 are separated by 143 nucleotides GFF7033 - protein of unknown function DUF427, at 207 to 491 GFF7033 GFF7034 - FIG00538743: hypothetical protein, at 488 to 1,243 GFF7034 GFF7035 - UspA, at 1,387 to 1,827 GFF7035 Position (kb) 0 1Strain fitness (log2 ratio) -2 -1 0 1 2at 0.098 kb on - strandat 0.098 kb on - strandat 0.102 kb on - strandat 0.102 kb on - strandat 0.102 kb on - strandat 0.143 kb on - strandat 0.276 kb on + strand, within GFF7033at 0.276 kb on + strand, within GFF7033at 0.360 kb on + strand, within GFF7033at 0.360 kb on + strand, within GFF7033at 0.360 kb on + strand, within GFF7033at 0.360 kb on + strand, within GFF7033at 0.361 kb on - strand, within GFF7033at 0.363 kb on + strand, within GFF7033at 0.363 kb on + strand, within GFF7033at 0.363 kb on + strand, within GFF7033at 0.363 kb on + strand, within GFF7033at 0.363 kb on + strand, within GFF7033at 0.364 kb on - strand, within GFF7033at 0.364 kb on - strand, within GFF7033at 0.364 kb on - strand, within GFF7033at 0.408 kb on + strand, within GFF7033at 0.408 kb on + strand, within GFF7033at 0.409 kb on - strand, within GFF7033at 0.409 kb on - strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.411 kb on + strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.412 kb on - strand, within GFF7033at 0.560 kb on + strandat 0.560 kb on + strandat 0.561 kb on - strandat 0.561 kb on - strandat 0.561 kb on - strandat 0.779 kb on + strand, within GFF7034at 0.779 kb on + strand, within GFF7034at 0.779 kb on + strand, within GFF7034at 0.780 kb on - strand, within GFF7034at 0.780 kb on - strand, within GFF7034at 0.780 kb on - strand, within GFF7034at 0.780 kb on - strand, within GFF7034at 0.917 kb on + strand, within GFF7034at 0.917 kb on + strand, within GFF7034at 0.917 kb on + strand, within GFF7034at 0.918 kb on - strand, within GFF7034at 0.918 kb on - strand, within GFF7034at 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.297 kb on + strandat 1.298 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
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98 - -0.8
98 - -1.6
102 - +0.3
102 - -0.2
102 - -0.3
143 - -0.4
276 + GFF7033 0.24 +1.1
276 + GFF7033 0.24 +0.1
360 + GFF7033 0.54 -0.8
360 + GFF7033 0.54 +0.8
360 + GFF7033 0.54 +0.4
360 + GFF7033 0.54 +0.6
361 - GFF7033 0.54 +0.1
363 + GFF7033 0.55 +0.3
363 + GFF7033 0.55 -0.2
363 + GFF7033 0.55 -0.9
363 + GFF7033 0.55 -0.6
363 + GFF7033 0.55 -0.8
364 - GFF7033 0.55 +0.4
364 - GFF7033 0.55 +1.7
364 - GFF7033 0.55 -0.4
408 + GFF7033 0.71 +0.0
408 + GFF7033 0.71 -0.1
409 - GFF7033 0.71 +0.3
409 - GFF7033 0.71 +1.4
411 + GFF7033 0.72 -1.2
411 + GFF7033 0.72 +2.0
411 + GFF7033 0.72 +1.7
411 + GFF7033 0.72 +0.5
411 + GFF7033 0.72 -0.5
411 + GFF7033 0.72 +0.6
411 + GFF7033 0.72 -0.2
411 + GFF7033 0.72 -0.4
412 - GFF7033 0.72 -0.7
412 - GFF7033 0.72 -1.8
412 - GFF7033 0.72 -0.7
412 - GFF7033 0.72 -0.1
412 - GFF7033 0.72 -0.6
412 - GFF7033 0.72 -0.3
560 + -0.0
560 + +0.1
561 - +0.5
561 - -0.2
561 - +0.2
779 + GFF7034 0.38 -0.2
779 + GFF7034 0.38 -2.3
779 + GFF7034 0.38 -0.5
780 - GFF7034 0.39 -1.6
780 - GFF7034 0.39 +0.7
780 - GFF7034 0.39 -0.3
780 - GFF7034 0.39 -0.6
917 + GFF7034 0.57 -2.3
917 + GFF7034 0.57 -1.4
917 + GFF7034 0.57 -0.1
918 - GFF7034 0.57 +1.3
918 - GFF7034 0.57 -0.7
1,297 + -0.1
1,297 + -1.3
1,297 + -0.2
1,297 + -1.1
1,297 + +0.2
1,297 + +0.0
1,297 + -0.9
1,297 + +0.2
1,297 + +0.1
1,297 + -1.9
1,298 - -0.2

Or see this region's nucleotide sequence