Experiment: M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF6746 and GFF6747 overlap by 4 nucleotides GFF6747 and GFF6748 overlap by 4 nucleotides GFF6748 and GFF6749 are separated by 161 nucleotides
GFF6746 - dolichyl-phosphate-mannose-protein mannosyltransferase family protein, at 59,531 to 61,147
GFF6746
GFF6747 - Glycosyl transferase, family 2, at 61,144 to 62,229
GFF6747
GFF6748 - Undecaprenyl-diphosphatase BcrC (EC 3.6.1.27), conveys bacitracin resistance, at 62,226 to 62,810
GFF6748
GFF6749 - hypothetical protein, at 62,972 to 63,421
GFF6749
Position (kb)
61
62
63 Strain fitness (log2 ratio)
-1
0
1
2 at 60.342 kb on - strand, within GFF6746 at 60.342 kb on - strand, within GFF6746 at 60.342 kb on - strand, within GFF6746 at 60.345 kb on + strand, within GFF6746 at 60.345 kb on + strand, within GFF6746 at 60.346 kb on - strand, within GFF6746 at 60.346 kb on - strand, within GFF6746 at 60.417 kb on + strand, within GFF6746 at 60.417 kb on + strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.418 kb on - strand, within GFF6746 at 60.436 kb on - strand, within GFF6746 at 60.621 kb on + strand, within GFF6746 at 60.622 kb on - strand, within GFF6746 at 60.930 kb on + strand, within GFF6746 at 60.930 kb on + strand, within GFF6746 at 60.931 kb on - strand, within GFF6746 at 61.199 kb on + strand at 61.199 kb on + strand at 61.199 kb on + strand at 61.226 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.241 kb on + strand at 61.242 kb on - strand at 61.358 kb on + strand, within GFF6747 at 61.358 kb on + strand, within GFF6747 at 61.359 kb on - strand, within GFF6747 at 61.553 kb on + strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.554 kb on - strand, within GFF6747 at 61.682 kb on + strand, within GFF6747 at 61.682 kb on + strand, within GFF6747 at 61.683 kb on - strand, within GFF6747 at 61.683 kb on - strand, within GFF6747 at 61.745 kb on + strand, within GFF6747 at 61.745 kb on + strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 61.746 kb on - strand, within GFF6747 at 62.063 kb on + strand, within GFF6747 at 62.064 kb on - strand, within GFF6747 at 62.542 kb on + strand, within GFF6748 at 62.543 kb on - strand, within GFF6748 at 62.611 kb on - strand, within GFF6748 at 62.650 kb on + strand, within GFF6748 at 62.650 kb on + strand, within GFF6748 at 62.650 kb on + strand, within GFF6748 at 62.879 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction M9 with calcium pantothenate 10 mg/mL and thiamine HCl 50 mg/mL remove 60,342 - GFF6746 0.50 +2.6 60,342 - GFF6746 0.50 -0.2 60,342 - GFF6746 0.50 +0.1 60,345 + GFF6746 0.50 -0.1 60,345 + GFF6746 0.50 +0.2 60,346 - GFF6746 0.50 +0.3 60,346 - GFF6746 0.50 -0.5 60,417 + GFF6746 0.55 -0.6 60,417 + GFF6746 0.55 -0.8 60,418 - GFF6746 0.55 -0.3 60,418 - GFF6746 0.55 -0.2 60,418 - GFF6746 0.55 -0.1 60,436 - GFF6746 0.56 +0.5 60,621 + GFF6746 0.67 -0.2 60,622 - GFF6746 0.67 +0.6 60,930 + GFF6746 0.87 +0.6 60,930 + GFF6746 0.87 +0.4 60,931 - GFF6746 0.87 -0.8 61,199 + -0.3 61,199 + -0.0 61,199 + -0.6 61,226 + +0.5 61,241 + -1.0 61,241 + +1.1 61,241 + -0.5 61,241 + -0.7 61,242 - -0.3 61,358 + GFF6747 0.20 +1.0 61,358 + GFF6747 0.20 -0.5 61,359 - GFF6747 0.20 +0.8 61,553 + GFF6747 0.38 +0.6 61,554 - GFF6747 0.38 +0.6 61,554 - GFF6747 0.38 +0.1 61,554 - GFF6747 0.38 +0.4 61,554 - GFF6747 0.38 +0.9 61,554 - GFF6747 0.38 +0.1 61,682 + GFF6747 0.50 +0.1 61,682 + GFF6747 0.50 -0.6 61,683 - GFF6747 0.50 -0.9 61,683 - GFF6747 0.50 +0.6 61,745 + GFF6747 0.55 -0.2 61,745 + GFF6747 0.55 -0.6 61,746 - GFF6747 0.55 -0.4 61,746 - GFF6747 0.55 +0.6 61,746 - GFF6747 0.55 -0.5 62,063 + GFF6747 0.85 -0.1 62,064 - GFF6747 0.85 +0.6 62,542 + GFF6748 0.54 +0.7 62,543 - GFF6748 0.54 -0.3 62,611 - GFF6748 0.66 -1.0 62,650 + GFF6748 0.72 -0.1 62,650 + GFF6748 0.72 +0.3 62,650 + GFF6748 0.72 -0.6 62,879 + +1.2
Or see this region's nucleotide sequence